Acellular dermal matrix from one-day-old mouse skin on adult scarless cutaneous wound repair by second harmonic generation microscopic imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (76) ◽  
pp. 71852-71862 ◽  
Author(s):  
Xue Han ◽  
Hanping Liu ◽  
Maosheng Chen ◽  
Li Gong ◽  
Hongwen Pang ◽  
...  
Keyword(s):  
Wound Repair ◽  
Second Harmonic ◽  
Mouse Skin ◽  
Acellular Dermal Matrix ◽  
Cutaneous Wound Healing ◽  
Dermal Matrix ◽  
Microscopic Imaging ◽  
Cutaneous Wound ◽  
Collagen Density ◽  
Acellular Dermal

The impacts of two types of acellular dermal matrix (ADM), ADM-1D and ADM-20W (ADM from 1-day-old and 20-week-old mouse skin), are evaluated on collagen density, orientation and the stiffness of new born dermis in adult cutaneous wound healing.

scholarly journals MSCs on an acellular dermal matrix (ADM) sourced from neonatal mouse skin regulate collagen reconstruction of granulation tissue during adult cutaneous wound healing

RSC Advances ◽  
2017 ◽  
Vol 7 (37) ◽  
pp. 22998-23010 ◽  
Author(s):  
Maosheng Chen ◽  
Ying Jin ◽  
Xue Han ◽  
Ning Wang ◽  
Xiaoyuan Deng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Skin ◽  
Acellular Dermal Matrix ◽  
Neonatal Mouse ◽  
The Novel ◽  
Cutaneous Wound Healing ◽  
Dermal Matrix ◽  
Cutaneous Wound ◽  
Novel Strategy ◽  
Acellular Dermal

The novel strategy of MSCs seeded on ADM sourced from neonatal mouse skin promotes full-thickness cutaneous wound healing.

scholarly journals Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques

2017 ◽  
Vol 42 (2) ◽  
pp. 623-639 ◽  
Author(s):  
Ning Wang ◽  
Hanping Liu ◽  
Xuefeng Li ◽  
Qilei Zhang ◽  
Maosheng Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Transgenic Mice ◽  
Second Harmonic ◽  
Smooth Muscle Actin ◽  
Cutaneous Wound Healing ◽  
Post Transplantation ◽  
Dermal Matrix ◽  
Double Labeling ◽  
Cutaneous Wound ◽  
Cell Numbers

Background/Aims: Bone marrow Mesenchymal stem cells (MSCs) are promising for promoting cutaneous wound healing through reinforcing cellular processes. We evaluated the effect of GFP-tagged MSCs transplantation on skin regeneration in excisional wounds in mice. Methods: MSCs from GFP-labeled transgenic mice were co-cultured with acellular dermal matrix (ADM) scaffolds, and MSC-ADM scaffolds were transplanted into surgical skin wounds of BALB/c mice. After implantation, the survival and behavior of MSCs were examined by second harmonic generation and two-photon excitation fluorescence imaging, western blotting and DNA amplification and sequencing. Results: GFP-tagged MSCs were retained inside the regenerating skin until day 14 post-transplantation. Alpha-smooth muscle actin (α-SMA) and vimentin (VIM) were detected at 3, 5, 7, and 14 days post-transplantation by immunofluorescence double labeling. Although the GFP+/α-SMA+- and GFP+/VIM+-cell numbers decreased gradually with healing time, α-SMA+- and VIM+-cell numbers significantly increased, most of them were endogenous functional cells which were related to angiogenesis and collagen fiber structural remodeling. Conclusion: Therefore, in the initial stage of wound healing, transplanted MSCs differentiated into functional cells and played paracrine roles to recruit more endogenous cells for tissue remodeling. With the disappearance of exogenous cells, endogenous cells were responsible for the latter stage of cutaneous wound healing.

scholarly journals Acellular dermal matrix as filler in breast-conserving surgery: warnings for a careful use

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Gianluca Franceschini ◽  
Riccardo Masetti
Keyword(s):  
Acellular Dermal Matrix ◽  
Breast Conserving Surgery ◽  
Human Origin ◽  
Dermal Matrix ◽  
Adequate Knowledge ◽  
Aesthetic Results ◽  
Dermal Matrices ◽  
Acellular Dermal ◽  
Human Acellular Dermal Matrix ◽  
Host Tissues

AbstractAcellular dermal matrices are biological materials of porcine, bovine, or human origin used as scaffold for reconstructive purpose in plastic surgery; these materials are well-tolerated and safely integrated in host tissues without causing resorption, contracture, and encapsulation thanks to their low antigenicity.Recently, human acellular dermal matrix has been used as a filler in breast-conserving surgery to improve aesthetic results. Adequate knowledge of biomaterials properties, appropriate skill, and careful compliance with some specific recommendations are mandatory in order to optimize outcomes and obtain a work of success.

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