Alveolar ridge preservation with fibroblast growth factor‐2 modified acellular dermal matrix membrane and a bovine‐derived xenograft. An experimental in vivo study.

2021 ◽  
Author(s):  
Ruolin Wang ◽  
Wenhua Liu ◽  
Hongmei Guo ◽  
Shaohua Ge ◽  
Haiyun Huang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Acellular Dermal Matrix ◽  
Fibroblast Growth Factor 2 ◽  
Ridge Preservation ◽  
Alveolar Ridge ◽  
Fibroblast Growth ◽  
Dermal Matrix ◽  
Alveolar Ridge Preservation ◽  
Acellular Dermal

scholarly journals Effect of Growth Factor-Loaded Acellular Dermal Matrix/MSCs on Regeneration of Chronic Tympanic Membrane Perforations in Rats

2021 ◽  
Vol 10 (7) ◽  
pp. 1541
Author(s):  
Gwang-Won Cho ◽  
Changjong Moon ◽  
Anji Song ◽  
Karthikeyan A. Vijayakumar ◽  
Mary Jasmin Ang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Success Rate ◽  
Tympanic Membrane ◽  
Acellular Dermal Matrix ◽  
In Vivo Studies ◽  
Dermal Matrix ◽  
Tympanic Membrane Perforations ◽  
Acellular Dermal

The success rate of grafting using acellular dermal matrix (ADM) for chronic tympanic membrane was reported in previous studies to be lower than fascia or perichondrium. Combining mesenchymal stem cells (MSCs) and growth factor-loaded ADM for the regeneration of chronic TMP has not been reported so far. In this study, we hypothesized that combining growth factor-loaded ADM/MSCs could promote the recruitment of MSCs and assist in TMP regeneration. We evaluated the regeneration and compared the performance of four scaffolds in both in vitro and in vivo studies. MTT, qPCR, and immunoblotting were performed with MSCs. In vivo study was conducted in 4 groups (control; ADM only, ADM/MSC, ADM/MSC/bFGF, ADM/MSC/EGF) of rats and inferences were made by otoendoscopy and histological changes. Attachment of MSCs on ADM was observed by confocal microscopy. Proliferation rate increased with time in all treated cells. Regeneration-related gene expression in the treated groups was higher. Also, graft success rate was significantly higher in ADM/MSC/EGF group than other groups. Significant relationships were disclosed in neodrum thickness between each group. The results suggest, in future, combining EGF with ADM/MSCs could possibly be used as an outpatient treatment, without the need for surgery for eardrum regeneration.

scholarly journals Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture

2021 ◽  
Author(s):  
Eishin Yaoita ◽  
Masaaki Nameta ◽  
Yutaka Yoshida ◽  
Hidehiko Fujinaka
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 2 ◽  
Quiescent State ◽  
Fibroblast Growth ◽  
Gene Expressions ◽  
Dynamic Changes ◽  
Ki 67

AbstractFibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo.

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