scholarly journals MSCs on an acellular dermal matrix (ADM) sourced from neonatal mouse skin regulate collagen reconstruction of granulation tissue during adult cutaneous wound healing

RSC Advances ◽  
2017 ◽  
Vol 7 (37) ◽  
pp. 22998-23010 ◽  
Author(s):  
Maosheng Chen ◽  
Ying Jin ◽  
Xue Han ◽  
Ning Wang ◽  
Xiaoyuan Deng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Skin ◽  
Acellular Dermal Matrix ◽  
Neonatal Mouse ◽  
The Novel ◽  
Cutaneous Wound Healing ◽  
Dermal Matrix ◽  
Cutaneous Wound ◽  
Novel Strategy ◽  
Acellular Dermal

The novel strategy of MSCs seeded on ADM sourced from neonatal mouse skin promotes full-thickness cutaneous wound healing.

Acellular dermal matrix from one-day-old mouse skin on adult scarless cutaneous wound repair by second harmonic generation microscopic imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (76) ◽  
pp. 71852-71862 ◽  
Author(s):  
Xue Han ◽  
Hanping Liu ◽  
Maosheng Chen ◽  
Li Gong ◽  
Hongwen Pang ◽  
...  
Keyword(s):  
Wound Repair ◽  
Second Harmonic ◽  
Mouse Skin ◽  
Acellular Dermal Matrix ◽  
Cutaneous Wound Healing ◽  
Dermal Matrix ◽  
Microscopic Imaging ◽  
Cutaneous Wound ◽  
Collagen Density ◽  
Acellular Dermal

The impacts of two types of acellular dermal matrix (ADM), ADM-1D and ADM-20W (ADM from 1-day-old and 20-week-old mouse skin), are evaluated on collagen density, orientation and the stiffness of new born dermis in adult cutaneous wound healing.

Application of paste-type acellular dermal matrix in hard-to-heal wounds

2021 ◽  
Vol 30 (5) ◽  
pp. 414-418
Author(s):  
Sang Wha Kim ◽  
Hyung Sup Shim ◽  
Jihye Lee ◽  
Youn Hwan Kim
Keyword(s):  
Wound Healing ◽  
Adverse Events ◽  
Acellular Dermal Matrix ◽  
Complete Healing ◽  
Dermal Matrix ◽  
Wound Area ◽  
Number Of Patients ◽  
Complete Wound Healing ◽  
Zero Baseline ◽  
Acellular Dermal

Objective: The extracellular matrix (ECM) is one of the most important elements in wound healing. Absence or dysfunction of the ECM may impair wound healing. The application of acellular dermal matrix (ADM) as a substitute for ECM has been suggested. This study investigated the clinical application and wound healing effects of a paste-type ADM in patients presenting with hard-to-heal wounds due to various causes. Method: Patients with a hard-to-heal wound for >1 month, from September 2017 to February 2019, were included in this study. After debridement, the paste-type ADM was applied, at zero (baseline), two and four weeks. After application of the paste-type ADM, a conventional dressing was applied using polyurethane foam. Wound size, the formation of granulation tissue, re-epithelialisation, complete healing and adverse events were recorded at zero (baseline), one, two, four, eight and 12 weeks after the initial treatment. Results: A total of 18 patients took part (eight male, 10 female, mean age of 56±16.16 years). The mean wound area decreased from 17.42±10.04cm2 to 12.73±7.60cm2 by week one (p<0.05), to 10.16±7.00 by week two (p<0.0005), to 5.56±5.25 by week four (p<0.0001), to 2.77±5.15 by week eight (p<0.0001) and to 2.07±4.78 by week 12 (p<0.0001). The number of patients with >75% re-epithelialisation increased from two (11.1%) at two weeks to five (27.8%) at four weeks, to 11 (61.1%) at eight weeks and to 13 (72.2%) at 12 weeks. The number of patients showing complete wound healing was two (11.1%) at four weeks, nine (50.0%) at eight weeks and 12 (66.7%) at 12 weeks. No adverse events were reported during treatment. Conclusion: The paste-type ADM used in this study is a viable option for facilitating wound healing; it can shorten hospitalisation, and promote a faster recovery and return to normal life activities.

scholarly journals A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Wei Lin ◽  
Xiaoyang Qi ◽  
Wenjing Guo ◽  
Danyang Liang ◽  
Heting Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Delivery System ◽  
Acellular Dermal Matrix ◽  
Cell Delivery ◽  
Stem Cell Therapies ◽  
Dermal Matrix ◽  
Cell Therapies ◽  
Stem Cell Delivery ◽  
Acellular Dermal

Abstract Background Stem cell therapies have gained great attention for providing novel solutions for treatment of various injuries and diseases due to stem cells’ self-renewal, ability to differentiate into various cell types, and favorite paracrine function. Nevertheless, the low retention of transplanted stem cell still limits their clinical applications such as in wound healing in view of an induced harsh microenvironment rich in reactive oxygen species (ROS) during inflammatory reactions. Methods Herein, a novel chitosan/acellular dermal matrix (CHS/ADM) stem cell delivery system is developed, which is of great ROS scavenging activity and significantly attenuates inflammatory response. Result Under ROS microenvironment, this stem cell delivery system acts as a barrier, effectively scavenging an amount of ROS and protecting mesenchymal stem cells (MSCs) from the oxidative stress. It notably regulates intracellular ROS level in MSCs and reduces ROS-induced cellular death. Most importantly, such MSCs delivery system significantly enhances in vivo transplanted stem cell retention, promotes the vessel growth, and accelerates wound healing. Conclusions This novel delivery system, which overcomes the limitations of conventional plain collagen-based delivery system in lacking of ROS-environmental responsive mechanisms, demonstrates a great potential use in stem cell therapies in wound healing.

scholarly journals Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jin Liu ◽  
Xinyu Qiu ◽  
Yajie Lv ◽  
Chenxi Zheng ◽  
Yan Dong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Mouse Skin ◽  
Skin Wound ◽  
Therapeutic Effects ◽  
Cutaneous Wound Healing ◽  
Apoptotic Bodies ◽  
Skin Wound Healing ◽  
Cutaneous Wound

Abstract Background As the major interface between the body and the external environment, the skin is liable to various injuries. Skin injuries often lead to severe disability, and the exploration of promising therapeutic strategies is of great importance. Exogenous mesenchymal stem cell (MSC)-based therapy is a potential strategy due to the apparent therapeutic effects, while the underlying mechanism is still elusive. Interestingly, we observed the extensive apoptosis of exogenous bone marrow mesenchymal stem cells (BMMSCs) in a short time after transplantation in mouse skin wound healing models. Considering the roles of extracellular vesicles (EVs) in intercellular communication, we hypothesized that the numerous apoptotic bodies (ABs) released during apoptosis may partially contribute to the therapeutic effects. Methods ABs derived from MSCs were extracted, characterized, and applied in mouse skin wound healing models, and the therapeutic effects were evaluated. Then, the target cells of ABs were explored, and the effects of ABs on macrophages were investigated in vitro. Results We found ABs derived from MSCs promoted cutaneous wound healing via triggering the polarization of macrophages towards M2 phenotype. In addition, the functional converted macrophages further enhanced the migration and proliferation abilities of fibroblasts, which together facilitated the wound healing process. Conclusions Collectively, our study demonstrated that transplanted MSCs promoted cutaneous wound healing partially through releasing apoptotic bodies which could convert the macrophages towards an anti-inflammatory phenotype that plays a crucial role in the tissue repair process.

Adipose-Derived Stem Cells Seeded on Acellular Dermal Matrix Grafts Enhance Wound Healing in a Murine Model of a Full-Thickness Defect

2012 ◽  
Vol 69 (6) ◽  
pp. 656-662 ◽  
Author(s):  
Sheng-Ping Huang ◽  
Chia-Chun Hsu ◽  
Shun-Cheng Chang ◽  
Chih-Hsin Wang ◽  
Shou-Cheng Deng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Murine Model ◽  
Acellular Dermal Matrix ◽  
Adipose Derived Stem Cells ◽  
Full Thickness ◽  
Dermal Matrix ◽  
Acellular Dermal ◽  
Enhance Wound Healing

Denatured acellular dermal matrix seeded with bone marrow mesenchymal stem cells for wound healing in mice

Burns ◽  
2019 ◽  
Vol 45 (7) ◽  
pp. 1685-1694 ◽  
Author(s):  
Yongjun Qi ◽  
Zhengxue Dong ◽  
Hongzhen Chu ◽  
Qi Zhao ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Marrow Mesenchymal Stem Cells ◽  
Acellular Dermal

scholarly journals Exosomes derived from human adipose mensenchymal stem cells accelerates cutaneous wound healing via optimizing the characteristics of fibroblasts

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Li Hu ◽  
Juan Wang ◽  
Xin Zhou ◽  
Zehuan Xiong ◽  
Jiajia Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Soft Tissue ◽  
Early Stage ◽  
Mouse Skin ◽  
Scar Formation ◽  
Collagen I ◽  
Dependent Manner ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

Abstract Prolonged healing and scar formation are two major challenges in the treatment of soft tissue trauma. Adipose mesenchymal stem cells (ASCs) play an important role in tissue regeneration, and recent studies have suggested that exosomes secreted by stem cells may contribute to paracrine signaling. In this study, we investigated the roles of ASCs-derived exosomes (ASCs-Exos) in cutaneous wound healing. We found that ASCs-Exos could be taken up and internalized by fibroblasts to stimulate cell migration, proliferation and collagen synthesis in a dose-dependent manner, with increased genes expression of N-cadherin, cyclin-1, PCNA and collagen I, III. In vivo tracing experiments demonstrated that ASCs-Exos can be recruited to soft tissue wound area in a mouse skin incision model and significantly accelerated cutaneous wound healing. Histological analysis showed increased collagen I and III production by systemic administration of exosomes in the early stage of wound healing, while in the late stage, exosomes might inhibit collagen expression to reduce scar formation. Collectively, our findings indicate that ASCs-Exos can facilitate cutaneous wound healing via optimizing the characteristics of fibroblasts. Our results provide a new perspective and therapeutic strategy for the use of ASCs-Exos in soft tissue repair.

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