scholarly journals Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques

2017 ◽  
Vol 42 (2) ◽  
pp. 623-639 ◽  
Author(s):  
Ning Wang ◽  
Hanping Liu ◽  
Xuefeng Li ◽  
Qilei Zhang ◽  
Maosheng Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Transgenic Mice ◽  
Second Harmonic ◽  
Smooth Muscle Actin ◽  
Cutaneous Wound Healing ◽  
Post Transplantation ◽  
Dermal Matrix ◽  
Double Labeling ◽  
Cutaneous Wound ◽  
Cell Numbers

Background/Aims: Bone marrow Mesenchymal stem cells (MSCs) are promising for promoting cutaneous wound healing through reinforcing cellular processes. We evaluated the effect of GFP-tagged MSCs transplantation on skin regeneration in excisional wounds in mice. Methods: MSCs from GFP-labeled transgenic mice were co-cultured with acellular dermal matrix (ADM) scaffolds, and MSC-ADM scaffolds were transplanted into surgical skin wounds of BALB/c mice. After implantation, the survival and behavior of MSCs were examined by second harmonic generation and two-photon excitation fluorescence imaging, western blotting and DNA amplification and sequencing. Results: GFP-tagged MSCs were retained inside the regenerating skin until day 14 post-transplantation. Alpha-smooth muscle actin (α-SMA) and vimentin (VIM) were detected at 3, 5, 7, and 14 days post-transplantation by immunofluorescence double labeling. Although the GFP+/α-SMA+- and GFP+/VIM+-cell numbers decreased gradually with healing time, α-SMA+- and VIM+-cell numbers significantly increased, most of them were endogenous functional cells which were related to angiogenesis and collagen fiber structural remodeling. Conclusion: Therefore, in the initial stage of wound healing, transplanted MSCs differentiated into functional cells and played paracrine roles to recruit more endogenous cells for tissue remodeling. With the disappearance of exogenous cells, endogenous cells were responsible for the latter stage of cutaneous wound healing.

scholarly journals MSCs on an acellular dermal matrix (ADM) sourced from neonatal mouse skin regulate collagen reconstruction of granulation tissue during adult cutaneous wound healing

RSC Advances ◽  
2017 ◽  
Vol 7 (37) ◽  
pp. 22998-23010 ◽  
Author(s):  
Maosheng Chen ◽  
Ying Jin ◽  
Xue Han ◽  
Ning Wang ◽  
Xiaoyuan Deng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Skin ◽  
Acellular Dermal Matrix ◽  
Neonatal Mouse ◽  
The Novel ◽  
Cutaneous Wound Healing ◽  
Dermal Matrix ◽  
Cutaneous Wound ◽  
Novel Strategy ◽  
Acellular Dermal

The novel strategy of MSCs seeded on ADM sourced from neonatal mouse skin promotes full-thickness cutaneous wound healing.

Acellular dermal matrix from one-day-old mouse skin on adult scarless cutaneous wound repair by second harmonic generation microscopic imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (76) ◽  
pp. 71852-71862 ◽  
Author(s):  
Xue Han ◽  
Hanping Liu ◽  
Maosheng Chen ◽  
Li Gong ◽  
Hongwen Pang ◽  
...  
Keyword(s):  
Wound Repair ◽  
Second Harmonic ◽  
Mouse Skin ◽  
Acellular Dermal Matrix ◽  
Cutaneous Wound Healing ◽  
Dermal Matrix ◽  
Microscopic Imaging ◽  
Cutaneous Wound ◽  
Collagen Density ◽  
Acellular Dermal

The impacts of two types of acellular dermal matrix (ADM), ADM-1D and ADM-20W (ADM from 1-day-old and 20-week-old mouse skin), are evaluated on collagen density, orientation and the stiffness of new born dermis in adult cutaneous wound healing.

scholarly journals Deficiency in the LIM-only protein Fhl2 impairs skin wound healing

2007 ◽  
Vol 177 (1) ◽  
pp. 163-172 ◽  
Author(s):  
Viktor Wixler ◽  
Stephanie Hirner ◽  
Judith M. Müller ◽  
Lucia Gullotti ◽  
Carola Will ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Nuclear Translocation ◽  
Smooth Muscle Actin ◽  
Signal Molecules ◽  
Cutaneous Wound Healing ◽  
Bioactive Lipids ◽  
Skin Wound Healing ◽  
Cutaneous Wound ◽  
Deficient Mice

After skin wounding, the repair process is initiated by the release of growth factors, cytokines, and bioactive lipids from injured vessels and coagulated platelets. These signal molecules induce synthesis and deposition of a provisional extracellular matrix, as well as fibroblast invasion into and contraction of the wounded area. We previously showed that sphingosine-1-phosphate (S1P) triggers a signal transduction cascade mediating nuclear translocation of the LIM-only protein Fhl2 in response to activation of the RhoA GTPase (Muller, J.M., U. Isele, E. Metzger, A. Rempel, M. Moser, A. Pscherer, T. Breyer, C. Holubarsch, R. Buettner, and R. Schule. 2000. EMBO J. 19:359–369; Muller, J.M., E. Metzger, H. Greschik, A.K. Bosserhoff, L. Mercep, R. Buettner, and R. Schule. 2002. EMBO J. 21:736–748.). We demonstrate impaired cutaneous wound healing in Fhl2-deficient mice rescued by transgenic expression of Fhl2. Furthermore, collagen contraction and cell migration are severely impaired in Fhl2-deficient cells. Consequently, we show that the expression of α-smooth muscle actin, which is regulated by Fhl2, is reduced and delayed in wounds of Fhl2-deficient mice and that the expression of p130Cas, which is essential for cell migration, is reduced in Fhl2-deficient cells. In summary, our data demonstrate a function of Fhl2 as a lipid-triggered signaling molecule in mesenchymal cells regulating their migration and contraction during cutaneous wound healing.

Study on the Effect of Cerium Oxide Nanocubes on Full-Thickness Cutaneous Wound Healing of Type 2 Diabetic Rats

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 643-P ◽  
Author(s):  
YANFEI HAN ◽  
LINDONG LI ◽  
YANJUN LIU ◽  
YOU WANG ◽  
CHUNHUA YAN ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cerium Oxide ◽  
Diabetic Rats ◽  
Cutaneous Wound Healing ◽  
Full Thickness ◽  
Cutaneous Wound ◽  
Type 2 Diabetic

scholarly journals Optical coherence tomography angiography monitors human cutaneous wound healing over time

2018 ◽  
Vol 8 (2) ◽  
pp. 135-150 ◽  
Author(s):  
Anthony J. Deegan ◽  
Wendy Wang ◽  
Shaojie Men ◽  
Yuandong Li ◽  
Shaozhen Song ◽  
...  
Keyword(s):  
Wound Healing ◽  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Optical Coherence ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Over Time

Sulfated glycosaminoglycans in decellularized placenta matrix as critical regulators for cutaneous wound healing

2021 ◽  
Vol 122 ◽  
pp. 199-210
Author(s):  
Chen Wang ◽  
Guoyun Li ◽  
Kaige Cui ◽  
Zihan Chai ◽  
Ziyu Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound
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