A highly sensitive glutamic acid biosensor based on the determination of NADH enzymically generated by l-glutamic dehydrogenase

RSC Advances ◽  
2016 ◽  
Vol 6 (51) ◽  
pp. 45829-45834 ◽  
Author(s):  
Xia Lin ◽  
Qinghong Wang ◽  
Shu Zhu ◽  
Juanjuan Xu ◽  
Qiao Xia ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Highly Sensitive ◽  
Glutamic Dehydrogenase

In this article, a sensitive and stereo-selective biosensor for l-glutamic acid (l-Glu) based on the electrochemiluminescence (ECL) of Ru(bpy)32+ has been designed by applying l-glutamic dehydrogenase (GLDH) for enzymatic generation of NADH in situ.

scholarly journals A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine.

1995 ◽  
Vol 43 (4) ◽  
pp. 347-352 ◽  
Author(s):  
H M Kerstens ◽  
P J Poddighe ◽  
A G Hanselaar
Keyword(s):  
In Situ Hybridization ◽  
Signal Amplification ◽  
Dna Probes ◽  
Bright Field ◽  
Archival Material ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Catalyzed Reporter Deposition

For amplification of in situ hybridization (ISH) signals, we describe a method using catalyzed reporter deposition (CARD). This amplification method is based on the deposition of biotinylated tyramine (BT) at the location of the DNA probe. The BT precipitate can then visualized with fluorochrome- or enzyme-labeled avidin. Both for bright-field ISH (BRISH) and for fluorescence ISH (FISH), the detection limit was highly increased. This method is especially suitable for visualization of very weak ISH signals, such as those obtained by ISH using locus-specific DNA probes. Furthermore, CARD amplification of ISH signals (CARD-ISH) is highly sensitive, rapid, flexible, and easy to implement. Successful application of CARD-ISH with locus-specific DNA probes on histological and cytological samples may improve the determination of structural chromosomal aberrations in archival material.

scholarly journals Highly sensitive electrochemical determination of captopril using CuO modified ITO electrode: the effect of in situ grown nanostructures over signal sensitivity

RSC Advances ◽  
2017 ◽  
Vol 7 (31) ◽  
pp. 19353-19362 ◽  
Author(s):  
Razium Ali Soomro ◽  
Mawada Mohamed Tunesi ◽  
Selcan Karakus ◽  
Nazar Kalwar
Keyword(s):  
Electrochemical Determination ◽  
Ito Electrode ◽  
Highly Sensitive ◽  
Signal Sensitivity ◽  
Electrochemical Signal

Influence of nanostructures distribution over electrochemical signal sensitivity.

scholarly journals In situ growth of α-Fe2O3 nanorod arrays on 3D carbon foam as an efficient binder-free electrode for highly sensitive and specific determination of nitrite

2017 ◽  
Vol 5 (9) ◽  
pp. 4726-4736 ◽  
Author(s):  
Yue Ma ◽  
Xiangyang Song ◽  
Xiao Ge ◽  
Haimin Zhang ◽  
Guozhong Wang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Carbon Foam ◽  
In Situ Growth ◽  
Nanorod Arrays ◽  
Real Water Samples ◽  
Highly Sensitive ◽  
Specific Determination ◽  
Binder Free

3D α-Fe2O3 nanorod arrays (NAs)/carbon foam (CF) architectures have been successfully fabricated as binder-free electrodes for the determination of nitrite, exhibiting high sensitivity and excellent specific recognition as well as feasibility in real water samples.

Rapid single-cell detection of pathogenic bacteria for in situ determination of food safety

2020 ◽  
Vol 12 (46) ◽  
pp. 5621-5627
Author(s):  
Hee-Jung Kim ◽  
Suk-Jung Choi
Keyword(s):  
Food Safety ◽  
Single Cell ◽  
Pathogenic Bacteria ◽  
Fluorescent Labeling ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Magnetic Capture ◽  
Highly Sensitive

A highly sensitive in situ method to detect bacterial pathogens was developed using magnetic capture particles and europium-fluorescent labeling particles.

The in situ growth of CuO nanostructures on an ITO substrate and its application as a highly sensitive electrode for the electrochemical determination of N-acetyl-l-cysteine

2017 ◽  
Vol 5 (10) ◽  
pp. 2708-2716 ◽  
Author(s):  
Mawada Mohamed Tunesi ◽  
Razium Ali Soomro ◽  
Ramazan Ozturk
Keyword(s):  
Electrochemical Determination ◽  
Electrochemical Sensing ◽  
In Situ Growth ◽  
Highly Sensitive ◽  
Sensitive Electrode

This study describes the application of a new ITO-based electrode for the electrochemical sensing of N-acetyl-l-cysteine (NAC).

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