Magnetic solid phase extraction coupled with in situ derivatization for the highly sensitive determination of acidic phytohormones in rice leaves by UPLC-MS/MS

The Analyst ◽  
2014 ◽  
Vol 139 (21) ◽  
pp. 5605-5613 ◽  
Author(s):  
Jiu-Feng Liu ◽  
Jun Ding ◽  
Bi-Feng Yuan ◽  
Yu-Qi Feng
Keyword(s):  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Phase Extraction ◽  
Highly Sensitive ◽  
Magnetic Solid ◽  
Sensitive Determination ◽  
Rice Leaves ◽  
In Situ Derivatization

scholarly journals Magnetic Solid-Phase Extraction and in Situ Derivatization for Determining Phytohormones and in Oilseeds by Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry

2020 ◽  
Author(s):  
Zhoufei Luo ◽  
Mengwei Xu ◽  
Ruozhong Wang ◽  
Xiubing Liu ◽  
Langtao Xiao
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Ultra Performance Liquid Chromatography ◽  
Phase Extraction ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Magnetic Solid

Abstract Background: Phytohormones are a group of naturally occurring signaling molecules which influence physiological processes of oil crops. Simultaneous determination of multiple phytohormones in oilseeds is still a challenge due to their trace concentrations, species diversity, and lipid interference. Therefore, a simple and selective method for the simultaneous determination of multiple phytohormones in oilseeds is urgently needed.Results: In this study, the Fe3O4@Ti3C2@β-CD nanoparticles were successfully synthesized and used for the first time as an adsorbent for the magnetic dispersive solid-phase extraction of phytohormones from oilseeds. The magnetic dispersive solid-phase extraction and in situ derivation by the addition of N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) were ingeniously combined. This efficient pre-treatment method integrated the extraction, purification, and derivatization processes into one single step. Several parameters affecting the efficiency of extraction and derivatization were evaluated. Under the optimized analysis conditions, satisfactory methodological performance was achieved. High linearities (R2 > 0.9928) at three spiked levels, as well as the low matrix effect (ranged from ­16.63 % to 17.06 %) and limits of detection (0.89-13.62 pg/mL) were also obtained. The intra and inter-day relative standard deviations (RSDs) were less than 13.7 % and 11.6 %, respectively. The recoveries were ranged from 80.4 % to 115.1 %. This method was successfully employed for analyzing 12 phytohormones in different oilseeds samples.Conclusion: A simple and sensitive method based on the magnetic solid phase extraction integrated with in situ derivations for the profiling of 12 phytohormones, including 9 gibberellins (GAs), indole-3-acetic acid (IAA), abscisic acid (ABA), and jasmonic acid (JA) in a single rapeseed seed was developed by using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).

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