Rapid single-cell detection of pathogenic bacteria for in situ determination of food safety

2020 ◽  
Vol 12 (46) ◽  
pp. 5621-5627
Author(s):  
Hee-Jung Kim ◽  
Suk-Jung Choi
Keyword(s):  
Food Safety ◽  
Single Cell ◽  
Pathogenic Bacteria ◽  
Fluorescent Labeling ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Magnetic Capture ◽  
Highly Sensitive

A highly sensitive in situ method to detect bacterial pathogens was developed using magnetic capture particles and europium-fluorescent labeling particles.

scholarly journals In situ single cell detection via microfluidic magnetic bead assay

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0172697 ◽  
Author(s):  
Fan Liu ◽  
Pawan KC ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Single Cell ◽  
Magnetic Bead ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Magnetic Bead Assay

scholarly journals Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs

2019 ◽  
Author(s):  
Narendra Kumar ◽  
Wenjian Wang ◽  
Juan C. Ortiz-Marquez ◽  
Matthew Catalano ◽  
Mason Gray ◽  
...  
Keyword(s):  
Single Cell ◽  
Pathogenic Bacteria ◽  
Field Effect Transistors ◽  
Bacterial Species ◽  
Resistant Bacteria ◽  
Cell Detection ◽  
Bacterial Cells ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Single Cell Detection

AbstractThe rapid increase in antibiotic resistant pathogenic bacteria has become a global threat, which besides the development of new drugs, requires rapid, cheap, scalable, and accurate diagnostics. Label free biosensors relying on electrochemical, mechanical, and mass based detection of whole bacterial cells have attempted to meet these requirements. However, the trade-off between selectivity and sensitivity of such sensors remains a key challenge. In particular, point-of-care diagnostics that are able to reduce and/or prevent unneeded antibiotic prescriptions require highly specific probes with sensitive and accurate transducers that can be miniaturized and multiplexed, and that are easy to operate and cheap. Towards achieving this goal, we present a number of advances in the use of graphene field effect transistors (G-FET) including the first use of peptide probes to electrically detect antibiotic resistant bacteria in a highly specific manner. In addition, we dramatically reduce the needed concentration for detection by employing dielectrophoresis for the first time in a G-FET, allowing us to monitor changes in the Dirac point due to individual bacterial cells. Specifically, we realized rapid binding of bacterial cells to a G-FET by electrical field guiding to the device to realize an overall 3 order of magnitude decrease in cell-concentration enabling a single-cell detection limit, and 9-fold reduction in needed time to 5 minutes. Utilizing our new biosensor and procedures, we demonstrate the first selective, electrical detection of the pathogenic bacterial species Staphylococcus aureus and antibiotic resistant Acinetobacter baumannii on a single platform.

A novel method for single cell detection of in situ telomerase or histone H3 in combination with clonal analysis by FISH

2003 ◽  
Vol 27 (6) ◽  
pp. 529-537
Author(s):  
Betty Gladstone ◽  
Smitha Sivaraman ◽  
Naomi Galili ◽  
Parameswaran Venugopal ◽  
Imke Janssen ◽  
...  
Keyword(s):  
Single Cell ◽  
Histone H3 ◽  
Clonal Analysis ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Novel Method

A highly sensitive glutamic acid biosensor based on the determination of NADH enzymically generated by l-glutamic dehydrogenase

RSC Advances ◽  
2016 ◽  
Vol 6 (51) ◽  
pp. 45829-45834 ◽  
Author(s):  
Xia Lin ◽  
Qinghong Wang ◽  
Shu Zhu ◽  
Juanjuan Xu ◽  
Qiao Xia ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Highly Sensitive ◽  
Glutamic Dehydrogenase

In this article, a sensitive and stereo-selective biosensor for l-glutamic acid (l-Glu) based on the electrochemiluminescence (ECL) of Ru(bpy)32+ has been designed by applying l-glutamic dehydrogenase (GLDH) for enzymatic generation of NADH in situ.

scholarly journals Fluorescence enhanced BA-LIFT for single cell detection and isolation

2020 ◽  
Vol 12 (2) ◽  
pp. 025019 ◽  
Author(s):  
A Marquez ◽  
M Gómez-Fontela ◽  
S Lauzurica ◽  
R Candorcio-Simón ◽  
D Munoz-Martin ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection

Single-Cell Detection of Trans-Splicing Ribozyme In Vivo Activity

2004 ◽  
Vol 126 (23) ◽  
pp. 7158-7159 ◽  
Author(s):  
Sumitaka Hasegawa ◽  
Jung W. Choi ◽  
Jianghong Rao
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Trans Splicing

The Role of Nanotechnology in Single-Cell Detection: A Review

2014 ◽  
Vol 10 (10) ◽  
pp. 2598-2619 ◽  
Author(s):  
Changling Wang ◽  
Yuxiang Zhang ◽  
Mingdian Xia ◽  
Xingxi Zhu ◽  
Shitao Qi ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection

Single-cell detection by cavity ring-down spectroscopy

2004 ◽  
Vol 85 (19) ◽  
pp. 4523 ◽  
Author(s):  
Peter B. Tarsa ◽  
Aislyn D. Wist ◽  
Paul Rabinowitz ◽  
Kevin K. Lehmann
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Cavity Ring Down Spectroscopy ◽  
Single Cell Detection ◽  
Cavity Ring Down
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