scholarly journals In situ growth of α-Fe2O3 nanorod arrays on 3D carbon foam as an efficient binder-free electrode for highly sensitive and specific determination of nitrite

2017 ◽  
Vol 5 (9) ◽  
pp. 4726-4736 ◽  
Author(s):  
Yue Ma ◽  
Xiangyang Song ◽  
Xiao Ge ◽  
Haimin Zhang ◽  
Guozhong Wang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Carbon Foam ◽  
In Situ Growth ◽  
Nanorod Arrays ◽  
Real Water Samples ◽  
Highly Sensitive ◽  
Specific Determination ◽  
Binder Free

3D α-Fe2O3 nanorod arrays (NAs)/carbon foam (CF) architectures have been successfully fabricated as binder-free electrodes for the determination of nitrite, exhibiting high sensitivity and excellent specific recognition as well as feasibility in real water samples.

The in situ growth of CuO nanostructures on an ITO substrate and its application as a highly sensitive electrode for the electrochemical determination of N-acetyl-l-cysteine

2017 ◽  
Vol 5 (10) ◽  
pp. 2708-2716 ◽  
Author(s):  
Mawada Mohamed Tunesi ◽  
Razium Ali Soomro ◽  
Ramazan Ozturk
Keyword(s):  
Electrochemical Determination ◽  
Electrochemical Sensing ◽  
In Situ Growth ◽  
Highly Sensitive ◽  
Sensitive Electrode

This study describes the application of a new ITO-based electrode for the electrochemical sensing of N-acetyl-l-cysteine (NAC).

In-situ growth of porous CoTe2 nanosheets array on 3D nickel foam for highly sensitive binder-free non-enzymatic glucose sensor

2021 ◽  
pp. 158642
Author(s):  
Amjad Farid ◽  
Lujun Pan ◽  
Muhammad Usman ◽  
I.A. Khan ◽  
Abdul Sammed Khan ◽  
...  
Keyword(s):  
Glucose Sensor ◽  
Nickel Foam ◽  
In Situ Growth ◽  
Highly Sensitive ◽  
Binder Free

scholarly journals Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lenka Ulrychová ◽  
Pavel Ostašov ◽  
Marta Chanová ◽  
Michael Mareš ◽  
Martin Horn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Schistosoma Mansoni ◽  
Rna Sequencing ◽  
Serine Proteases ◽  
Expression Patterns ◽  
High Sensitivity ◽  
Host Parasite Interactions ◽  
Highly Sensitive ◽  
Host Parasite

Abstract Background The blood flukes of genus Schistosoma are the causative agent of schistosomiasis, a parasitic disease that infects more than 200 million people worldwide. Proteases of schistosomes are involved in critical steps of host–parasite interactions and are promising therapeutic targets. We recently identified and characterized a group of S1 family Schistosoma mansoni serine proteases, including SmSP1 to SmSP5. Expression levels of some SmSPs in S. mansoni are low, and by standard genome sequencing technologies they are marginally detectable at the method threshold levels. Here, we report their spatial gene expression patterns in adult S. mansoni by the high-sensitivity localization assay. Methodology Highly sensitive fluorescence in situ RNA hybridization (FISH) was modified and used for the localization of mRNAs encoding individual SmSP proteases (including low-expressed SmSPs) in tissues of adult worms. High sensitivity was obtained due to specifically prepared tissue and probes in combination with the employment of a signal amplification approach. The assay method was validated by detecting the expression patterns of a set of relevant reference genes including SmCB1, SmPOP, SmTSP-2, and Sm29 with localization formerly determined by other techniques. Results FISH analysis revealed interesting expression patterns of SmSPs distributed in multiple tissues of S. mansoni adults. The expression patterns of individual SmSPs were distinct but in part overlapping and were consistent with existing transcriptome sequencing data. The exception were genes with significantly low expression, which were also localized in tissues where they had not previously been detected by RNA sequencing methods. In general, SmSPs were found in various tissues including reproductive organs, parenchymal cells, esophagus, and the tegumental surface. Conclusions The FISH-based assay provided spatial information about the expression of five SmSPs in adult S. mansoni females and males. This highly sensitive method allowed visualization of low-abundantly expressed genes that are below the detection limits of standard in situ hybridization or by RNA sequencing. Thus, this technical approach turned out to be suitable for sensitive localization studies and may also be applicable for other trematodes. The results suggest that SmSPs may play roles in diverse processes of the parasite. Certain SmSPs expressed at the surface may be involved in host–parasite interactions. Graphic abstract

A highly sensitive glutamic acid biosensor based on the determination of NADH enzymically generated by l-glutamic dehydrogenase

RSC Advances ◽  
2016 ◽  
Vol 6 (51) ◽  
pp. 45829-45834 ◽  
Author(s):  
Xia Lin ◽  
Qinghong Wang ◽  
Shu Zhu ◽  
Juanjuan Xu ◽  
Qiao Xia ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Highly Sensitive ◽  
Glutamic Dehydrogenase

In this article, a sensitive and stereo-selective biosensor for l-glutamic acid (l-Glu) based on the electrochemiluminescence (ECL) of Ru(bpy)32+ has been designed by applying l-glutamic dehydrogenase (GLDH) for enzymatic generation of NADH in situ.

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