Ternary covalent conjugate (antibody–gold nanoparticle–peroxidase) for signal enhancement in enzyme immunoassay

RSC Advances ◽  
2016 ◽  
Vol 6 (54) ◽  
pp. 48827-48833 ◽  
Author(s):  
M. M. Vdovenko ◽  
N. A. Byzova ◽  
A. V. Zherdev ◽  
B. B. Dzantiev ◽  
I. Yu. Sakharov
Keyword(s):  
Gold Nanoparticles ◽  
Horseradish Peroxidase ◽  
Enzyme Immunoassay ◽  
Gold Nanoparticle ◽  
Signal Enhancement ◽  
Enzyme Content ◽  
High Enzyme ◽  
Covalently Bound

A synthesis of a new horseradish peroxidase (HRP) conjugate and antibody covalently bound with gold nanoparticles (GNPs) with a high enzyme content was first developed.

scholarly journals Synthesis of Horseradish Peroxidase-Gold Nanoparticle Conjugate through Green Route

2020 ◽  
Vol 32 (5) ◽  
pp. 1243-1247
Author(s):  
Lovnish Siyal ◽  
Benu Kumar ◽  
Ranjit Kumar ◽  
Rachana Sahney
Keyword(s):  
Gold Nanoparticles ◽  
Enzyme Activity ◽  
Horseradish Peroxidase ◽  
Gold Nanoparticle ◽  
Catalytic Efficiency ◽  
Hydrodynamic Diameter ◽  
Green Route ◽  
Peroxidase Enzyme ◽  
Uv Visible ◽  
Average Hydrodynamic Diameter

Synthesis of horseradish peroxidase-gold nanoparticle conjugates (HRP-AuNPs) has been studied for the development of biofunctionalized gold nanoparticles (AuNPs) through biogenic route. Herein, horseradish peroxidase enzyme has been used to synthesize gold nanoparticles at room temperature in tricine buffer. The morphology and size distribution of HRP-AuNPs conjugates were obtained by different techniques including dynamic light scattering (DLS), UV-visible (UV-vis) spectrophotometry, scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). The enzyme activity of HRP-AuNP conjugate was compared with free enzyme to determine their catalytic efficiency. The results suggests that HRP-AuNP conjugates are monodisperse particles with average hydrodynamic diameter of 83.93 ± 2.1 nm, zeta potential of about -18.4 ± 1.1 mV and higher enzyme activity towards H2O2 as compared to free horseradish peroxidase. These biofunctionalized gold nanoparticles could act as tag or labeling agent for various applications.

Interference of Gold Nanoparticles with In vitro Endotoxin Detection Assays

2020 ◽  
Vol 16 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Melissa A. Vetten ◽  
Mary Gulumian
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Surface Reactivity ◽  
In Vitro Assays ◽  
Limulus Amebocyte Lysate ◽  
Chromogenic Assay ◽  
Nanoparticle Suspensions ◽  
Endotoxin Contamination ◽  
Factor C

Background: Endotoxin-free engineered nanoparticle suspensions are imperative for their successful applications in the field of nanomedicine as well as in the investigations in their toxicity. Gold nanoparticles are known to interfere with various in vitro assays due to their optical properties and potential for surface reactivity. In vitro endotoxin testing assays are known to be susceptible to interference caused by the sample being tested. Objective: This study aimed to identify a preferred assay for the testing of endotoxin contamination in gold nanoparticle suspensions. Methods: The interference by gold nanoparticles on three assays namely, the commonly used limulus amebocyte lysate chromogenic assay, the limulus amebocyte lysate gel-clot method, and the less common recombinant Factor C (rFC) assay, was tested. Results: Possible interference could be observed with all three assays. The interference with the absorbance- based chromogenic assay could not be overcome by dilution; whilst the qualitative nature of the gel-clot assay excluded the possibility of distinguishing between a false positive result due to enhancement of the sensitivity of the assay, and genuine endotoxin contamination. However, interference with the rFC assay was easily overcome through dilution. Conclusion: The rFC assay is recommended as an option for endotoxin contamination detection in gold nanoparticle suspensions.

scholarly journals CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Nanoparticle Aggregation ◽  
Cleavage Activity ◽  
Gold Nanoparticle Aggregation

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...

Fluorescence biosensor for Salmonella typhimurium detection in food based on nano-self-assembly of alendronic acid modified upconversion and gold nanoparticles

2021 ◽  
Author(s):  
Min Chen ◽  
Leiqing Pan ◽  
K. Tu
Keyword(s):  
Gold Nanoparticles ◽  
Salmonella Typhimurium ◽  
Gold Nanoparticle ◽  
Alendronic Acid ◽  
Self Assembly ◽  
Upconversion Nanoparticles ◽  
Fluorescent Biosensor ◽  
Fluorescence Biosensor

A simple and quick responsive fluorescent biosensor for Salmonella typhimurium detection based on the recognition of aptamer coupled with alendronic acid (ADA)@upconversion nanoparticles (UCNPs) and gold nanoparticle (AuNPs) has been...

scholarly journals Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Metals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 472
Author(s):  
Qunying Yuan ◽  
Manjula Bomma ◽  
Zhigang Xiao
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Synthesis Method ◽  
Nanoparticle Synthesis ◽  
Morphological Properties ◽  
Phytochelatin Synthase ◽  
Rhizobium Tropici ◽  
Whole Cells ◽  
E Coli ◽  
Recombinant Cells

Phytochelatins, the enzymatic products of phytochelatin synthase, play a principal role in protecting the plants from heavy metal and metalloid toxicity due to their ability to scavenge metal ions. In the present study, we investigated the capacity of soluble intracellular extracts from E. coli cells expressing R. tropici phytochelatin synthase to synthesize gold nanoparticle. We discovered that the reaction mediated by soluble extracts from the recombinant E. coli cells had a higher yield of gold nanoparticles, compared to that from the control cells. The compositional and morphological properties of the gold nanoparticles synthesized by the intracellular extracts from recombinant cells and control cells were similar. In addition, this extracellular nanoparticle synthesis method produced purer gold nanoparticles, avoiding the isolation of nanoparticles from cellular debris when whole cells are used to synthesize nanoparticles. Our results suggested that phytochelatins can improve the efficiency of gold nanoparticle synthesis mediated by bacterial soluble intracellular extracts, and the potential of extracellular nanoparticle synthesis platform for the production of nanoparticles in large quantity and pure form is worth further investigation.

scholarly journals Enhanced enzymatic activity from phosphotriesterase trimer gold nanoparticle bioconjugates for pesticide detection

The Analyst ◽  
2017 ◽  
Vol 142 (17) ◽  
pp. 3261-3271 ◽  
Author(s):  
John A. Hondred ◽  
Joyce C. Breger ◽  
Nate T. Garland ◽  
Eunkeu Oh ◽  
Kimihiro Susumu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Enzymatic Activity ◽  
Gold Nanoparticle ◽  
Pesticide Detection

A significant enzymatic enhancement of phosphotriesterase has been demonstrated when immobilized on various sized gold nanoparticles.

scholarly journals Correction: Investigation of plasmonic signal enhancement based on long range surface plasmon resonance with gold nanoparticle tags

2016 ◽  
Vol 4 (44) ◽  
pp. 10562-10562
Author(s):  
Chih-Tsung Yang ◽  
Lin Wu ◽  
Ping Bai ◽  
Benjamin Thierry
Keyword(s):  
Surface Plasmon Resonance ◽  
Gold Nanoparticle ◽  
Long Range ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Enhancement

Correction for ‘Investigation of plasmonic signal enhancement based on long range surface plasmon resonance with gold nanoparticle tags’ by Chih-Tsung Yang et al., J. Mater. Chem. C, 2016, 4, 9897–9904.

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