Signal Enhancement of Surface Plasmon Resonance Based on Gold Nanoparticle-Antibody Complex for Immunoassay

2006 ◽  
Vol 6 (11) ◽  
pp. 3521-3525 ◽  
Author(s):  
Woochang Lee ◽  
Byung-Keun Oh ◽  
Yong-Wan Kim ◽  
Jeong-Woo Choi
Keyword(s):  
Surface Plasmon Resonance ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Enhancement ◽  
Antibody Complex

scholarly journals Correction: Investigation of plasmonic signal enhancement based on long range surface plasmon resonance with gold nanoparticle tags

2016 ◽  
Vol 4 (44) ◽  
pp. 10562-10562
Author(s):  
Chih-Tsung Yang ◽  
Lin Wu ◽  
Ping Bai ◽  
Benjamin Thierry
Keyword(s):  
Surface Plasmon Resonance ◽  
Gold Nanoparticle ◽  
Long Range ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Enhancement

Correction for ‘Investigation of plasmonic signal enhancement based on long range surface plasmon resonance with gold nanoparticle tags’ by Chih-Tsung Yang et al., J. Mater. Chem. C, 2016, 4, 9897–9904.

Signal Enhancement of Surface Plasmon Resonance Based on Gold Nanoparticle-Antibody Complex for Immunoassay

2006 ◽  
Vol 6 (11) ◽  
pp. 3521-3525
Author(s):  
Woochang Lee ◽  
Byung-Keun Oh ◽  
Yong-Wan Kim ◽  
Jeong-Woo Choi
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Antibody Fragment ◽  
Signal Enhancement ◽  
Au Nanoparticle ◽  
Constant Size ◽  
Antibody Complex ◽  
Antibody Conjugate ◽  
Versus Antigen

In the immunoassay based on surface plasmon resonance (SPR) system, the signal enhancement was done by means of the conjugate of gold (Au) nanoparticle-antibody fragment. Antibody fragment was prepared for the improved immobilization based on Au-thiol interaction. Through the ellipsometric analysis on surface, the conjugation between Au and antibody fragment was performed in the oriented manner. The optimal fabrication conditions such as concentration and incubation time were determined for the constant size of the fabricated nanoparticle-antibody conjugate. Through the plot of SPR angle difference versus antigen concentration, the linear correlation was achieved, of which the detection limit was 100 fg/ml.

Signal Enhancement of Surface Plasmon Resonance Imaging for Detection of Acidovorax avenae subsp. citrulli

2015 ◽  
Vol 1131 ◽  
pp. 88-94
Author(s):  
Chokchai Puttharugsa ◽  
Oraprapai Gajanandana ◽  
Orawan Himananto ◽  
Ratthasart Amarit ◽  
Armote Somboonkaew ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Signal Enhancement ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Self Assembled Monolayer ◽  
Spr Imaging

Signal enhancement based surface plasmon resonance imaging (SPR imaging) was developed using gold nanoparticle (AuNP) for detection of Acidovorax avenae subsp. citrulli (Aac). The antibodies against Aac, monoclonal antibody 11E5 (MAb 11E5) and polyclonal antibody MPC (PAb MPC), were covalently immobilized on the 1:40 of mixed self-assembled monolayer (mixed SAMs) surface for detection of Aac. The 107 cfu/ml of Aac was injected over the surface and was captured by immobilized antibodies on the sensing surface. PAb MPC conjugated to 10 nm of gold nanoparticle (PAb-AuNP) was flowed over the surface to enhance the SPR signal for detection of Aac. The MAb/Aac/PAb-AuNP assay provides a higher in signal enhancement than that of PAb/Aac/PAb-AuNP assay. Moreover, SPR signal using PAb-AuNP enhancement increases 23 – fold in signal enhancement when comparing to PAb enhancement. Thus, the detection of Aac based SPR imaging can be used PAb-AuNP in signal enhancement for further improvement in limit of detection (LOD).

Investigation of plasmonic signal enhancement based on long range surface plasmon resonance with gold nanoparticle tags

2016 ◽  
Vol 4 (41) ◽  
pp. 9897-9904 ◽  
Author(s):  
Chih-Tsung Yang ◽  
Lin Wu ◽  
Ping Bai ◽  
Benjamin Thierry
Keyword(s):  
Surface Plasmon Resonance ◽  
Gold Nanoparticle ◽  
Long Range ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Enhancement

Gold nanoparticle (AuNP) molecular tags yield a significant signal enhancement in long range SPR-based biosensing.

Surface Plasmon Resonance Enhanced Real-Time Photoelectrochemical Protein Sensing by Gold Nanoparticle-Decorated TiO2 Nanowires

2014 ◽  
Vol 86 (13) ◽  
pp. 6633-6639 ◽  
Author(s):  
Peimei Da ◽  
Wenjie Li ◽  
Xuan Lin ◽  
Yongcheng Wang ◽  
Jing Tang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Tio2 Nanowires ◽  
Protein Sensing
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