scholarly journals Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Metals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 472
Author(s):  
Qunying Yuan ◽  
Manjula Bomma ◽  
Zhigang Xiao
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Synthesis Method ◽  
Nanoparticle Synthesis ◽  
Morphological Properties ◽  
Phytochelatin Synthase ◽  
Rhizobium Tropici ◽  
Whole Cells ◽  
E Coli ◽  
Recombinant Cells

Phytochelatins, the enzymatic products of phytochelatin synthase, play a principal role in protecting the plants from heavy metal and metalloid toxicity due to their ability to scavenge metal ions. In the present study, we investigated the capacity of soluble intracellular extracts from E. coli cells expressing R. tropici phytochelatin synthase to synthesize gold nanoparticle. We discovered that the reaction mediated by soluble extracts from the recombinant E. coli cells had a higher yield of gold nanoparticles, compared to that from the control cells. The compositional and morphological properties of the gold nanoparticles synthesized by the intracellular extracts from recombinant cells and control cells were similar. In addition, this extracellular nanoparticle synthesis method produced purer gold nanoparticles, avoiding the isolation of nanoparticles from cellular debris when whole cells are used to synthesize nanoparticles. Our results suggested that phytochelatins can improve the efficiency of gold nanoparticle synthesis mediated by bacterial soluble intracellular extracts, and the potential of extracellular nanoparticle synthesis platform for the production of nanoparticles in large quantity and pure form is worth further investigation.

Green synthesis of gold nanoparticles using quail egg yolk and investigation of potential application areas

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Hayrunnisa Nadaroglu ◽  
Selvi Ince ◽  
Azize Alayli Gungor
Keyword(s):  
Gold Nanoparticles ◽  
Green Synthesis ◽  
High Pressures ◽  
Synthesis Method ◽  
Nanoparticle Synthesis ◽  
Egg Yolk ◽  
Morphological Properties ◽  
Scientific Development ◽  
Physical Methods ◽  
Egg Yolks

AbstractNanotechnology is a growing area of research. For example, gold nanoparticles have a wide variety of applications, including optical, electronic, and medical; they are also used as catalysts in biosensor applications and can be used for scanning, carrier, heat source, and sensors. Therefore, nanoparticle synthesis is of great importance to the country’s economy and to scientific development. Nanoparticle synthesis involves using chemical methods, physical methods, or both chemical and physical methods. The synthesis is performed at high pressures or high temperatures and severe conditions, both of which have a high cost in terms of energy. In our investigation, we used a green synthesis method, which used quail eggs that required more moderate conditions and less energy. The yolk from quail eggs has a high protein and vitamin content. Using quail egg yolks, the reaction conditions were optimized in terms of pH, temperature, and concentration. The morphological properties of the obtained gold nanoparticles were characterized using an ultraviolet-visible spectrophotometer, a scanning electron microscope, and an X-ray diffraction analysis.

Curcumin conjugated gold nanoparticle synthesis and its biocompatibility

RSC Advances ◽  
2014 ◽  
Vol 4 (4) ◽  
pp. 1808-1818 ◽  
Author(s):  
K. Sindhu ◽  
A. Rajaram ◽  
K. J. Sreeram ◽  
Rama Rajaram
Keyword(s):  
Gold Nanoparticles ◽  
Green Chemistry ◽  
Gold Nanoparticle ◽  
Nanoparticle Synthesis ◽  
Gold Nanoparticle Synthesis

Gold nanoparticles have gained much attention due to their widespread biological and technological applications, and consequently their simpler synthesis via green chemistry has also become of foremost importance.

scholarly journals Continuous flow synthesis of citrate capped gold nanoparticles using UV induced nucleation

RSC Advances ◽  
2017 ◽  
Vol 7 (16) ◽  
pp. 9632-9638 ◽  
Author(s):  
H. du Toit ◽  
T. J. Macdonald ◽  
H. Huang ◽  
I. P. Parkin ◽  
A. Gavriilidis
Keyword(s):  
Particle Size ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Continuous Flow ◽  
Nanoparticle Synthesis ◽  
Nucleation And Growth ◽  
Reactor System ◽  
Growth Phases ◽  
Flow Synthesis ◽  
Gold Nanoparticle Synthesis

A novel multimodal reactor system for separating the nucleation and growth phases of gold nanoparticle synthesis to control particle size.

The impact of the polar core size and external organic media composition on micelle–micelle interactions: the effect on gold nanoparticle synthesis

2015 ◽  
Vol 39 (11) ◽  
pp. 8887-8895 ◽  
Author(s):  
Jorge A. Gutierrez ◽  
M. Alejandra Luna ◽  
N. Mariano Correa ◽  
Juana J. Silber ◽  
R. Darío Falcone
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Reverse Micelles ◽  
Nanoparticle Synthesis ◽  
Organic Media ◽  
Core Size ◽  
Media Composition ◽  
Gold Nanoparticle Synthesis ◽  
The Impact

An easy way to modulate reverse micelles as nanoreactors to produce different kinds of gold nanoparticles.

scholarly journals Elucidating the Efficacy of Vaccination against Vibriosis in Lates calcarifer Using Two Recombinant Protein Vaccines Containing the Outer Membrane Protein K (r-OmpK) of Vibrio alginolyticus and the DNA Chaperone J (r-DnaJ) of Vibrio harveyi

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 660
Author(s):  
Santha Silvaraj ◽  
Ina Salwany Md Yasin ◽  
Murni Marlina A. Karim ◽  
Mohd Zamri Saad
Keyword(s):  
Adaptive Immune Response ◽  
Whole Cells ◽  
E Coli ◽  
Group 4 ◽  
Recombinant Cells ◽  
Antibody Titers ◽  
Recombinant Cell ◽  
Group 5 ◽  
Antibody Levels ◽  
Gut Lavage

Recombinant cell vaccines expressing the OmpK and DnaJ of Vibrio were developed and subsequently, a vaccination efficacy trial was carried out on juvenile seabass (~5 cm; ~20 g). The fish were divided into 5 groups of 50 fish per group, kept in triplicate. Groups 1 and 2 were injected with 107 CFU/mL of the inactivated recombinant cells vaccines, the pET-32/LIC-OmpK and pET-32/LIC-DnaJ, respectively. Group 3 was similarly injected with 107 CFU/mL of inactivated E. coli BL21 (DE3), Group 4 with 107 CFU/mL of formalin killed whole cells V. harveyi, and Group 5 with PBS solution. Serum, mucus, and gut lavage were used to determine the antibody levels before all fish were challenged with V. harveyi, V. alginolyticus, and V. parahemolyticus, respectively on day 15 post-vaccination. There was significant increase in the serum and gut lavage antibody titers in the juvenile seabass vaccinated with r-OmpK vaccine. In addition, there was an up-regulation for TLR2, MyD88, and MHCI genes in the kidney and intestinal tissues of r-OmpK vaccinated fish. At the same time, r-OmpK triggered higher expression level of interleukin IL-10, IL-8, IL-1ß in the spleen, intestine, and kidney compared to r-DnaJ. Overall, r-OmpK and r-DnaJ triggered protection by curbing inflammation and strengthening the adaptive immune response. Vaccinated fish also demonstrated strong cross protection against heterologous of Vibrio isolates, the V. harveyi, V. alginolyticus, and V. parahaemolyticus. The fish vaccinated with r-OmpK protein were completely protected with a relative per cent of survival (RPS) of 90 percent against V. harveyi and 100 percent against V. alginolyticus and V. parahaemolyticus. A semi-quantitative PCR detection of Vibrio spp. from the seawater containing the seabass also revealed that vaccination resulted in reduction of pathogen shedding. In conclusion, our results suggest r-OmpK as a candidate vaccine molecule against multiple Vibrio strain to prevent vibriosis in marine fish.

Optimization of Gold Nanoparticle Biosynthesis by Escherichia coli DH5α and its Conjugation with Gentamicin

2015 ◽  
Vol 32 ◽  
pp. 93-105 ◽  
Author(s):  
Hossein Motamedi ◽  
Samaneh Khademi Mazdeh ◽  
Azim Akbarzadeh Khiavi ◽  
Mohammad Reza Mehrabi
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Targeted Drug Delivery ◽  
Anaerobic Bacteria ◽  
Natural Resistance ◽  
In Vivo Studies ◽  
Optimum Conditions ◽  
E Coli ◽  
Nanoparticle Biosynthesis

Metal nanoparticles are one option for targeted drug delivery. In order to increase antibiotic efficiency and decrease its side effects, antibiotic conjugated nanoparticles have been known as a suitable approach. The aim of this study was optimization of gold nanoparticle biosynthesis byEscherichia coliDH5α and its conjugation with gentamicin. For this purpose gold nanoparticles were biosynthesized from HAuCl4and confirmed by Uv/ Vis, XRD, DLS and SEM. Then the effects of different parameters on optimum conditions for gold nanoparticles production were investigated. The MIC and MBC of gentamicin and its conjugate were investigated againstE. coli,Clostridium perfringensandClostridium botulinum. The results revealed that among different treatments, centrifuge (10000 rpm, 10 min) and sonication are the optimum conditions for gold nanoparticle production with less than 10 nm sizes. Filtration was also the best method for purifying nanoparticles. The conjugated nanoparticles significantly reduced the MIC of gentamicin againstE. coliand also overcame the natural resistance of tested anaerobic bacteria. In conclusion, the optimized method is an effective, inexpensive and environmental friendly method for biosynthesis of gold nanoparticles. Overcoming natural resistance of anaerobic bacteria using antibiotic conjugates with nanoparticles provides hopes for further experiments and in vivo studies.

scholarly journals Dyeing of cotton fabric materials with biogenic gold nanoparticles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Sivakavinesan ◽  
M. Vanaja ◽  
G. Annadurai
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Color Change ◽  
Nanoparticle Synthesis ◽  
Ion Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Fruit Peel ◽  
Garcinia Mangostana ◽  
Powder Concentration ◽  
Peel Extract

AbstractThe present work aimed at synthesizing gold nanoparticles in a biological method employing fruit peel waste dumped in the environment. The peels of Garcinia mangostana (Mangostan), were collected from the nearby tourist spot during the season. The collected fruit peels were washed, dried, powder and extracted by using boiling water and acetone. The precipitated extract was dried and powdered for further use. The dried and powdered peel extract was added to the gold solution and boiled to 80 °C and the color change is observed. The color change indicates the completion of the synthesis of gold nanoparticles. The effect of pH, gold ion concentration, peel extract powder concentration, and the temperature was tested by varying the parameters. The biosynthesized nanoparticles were characterized using the UV–Vis spectrophotometer to identify the surface plasmon resonance peaks corresponding to gold nanoparticles. The bio-moieties responsible for the synthesis of gold nanoparticles were identified using the Fourier Transform Infra-Red Spectroscopy. The crystalline nature was detected by using an X-Ray Diffractometer. Atomic Force Microscope viewed the 3D surface image of the gold nanoparticle. The shape and morphology of the nanoparticle were identified by using a Field Emission Scanning Electron Microscope. The active compounds for gold nanoparticle synthesis were identified using Gas Chromatography-Mass Spectrometry. The gold nanoparticle was synthesized in various colors and used for dyeing cotton fabrics. The dyed cotton materials were exposed to various stress conditions to determine the color fastening.

BIOSYNTHESIS AND CHARACTERIZATION OF GOLD NANOPARTICLES USING THE ALGA Kappaphycus alvarezii

2010 ◽  
Vol 09 (05) ◽  
pp. 511-516 ◽  
Author(s):  
P. RAJASULOCHANA ◽  
R. DHAMOTHARAN ◽  
P. MURUGAKOOTHAN ◽  
S. MURUGESAN ◽  
P. KRISHNAMOORTHY
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Kappaphycus Alvarezii ◽  
Nanoparticle Synthesis ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
Gold Nanoparticle Synthesis

As a part of our ongoing investigation into the use of algae for gold nanoparticle synthesis, we screened the marine alga Kappaphycus alvarezii, to investigate its efficiency to reduce gold ions as well as the formation of gold nanoparticles. In the present work, we report the reaction condition of the alga K. alvarezii with aqueous gold ions for gold nanoparticle synthesis within the biomass extracellularly. The formation of gold nanoparticles was characterized by UV–Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) method. Moreover, we have found that the reaction of gold ions with the K. alvarezii biomass under stationary conditions results in the rapid extracellular formation of gold nanoparticles of spherical morphology. The gold nanoparticles are not toxic to the cells that continued to grow after the biosynthesis of the gold nanoparticles.

scholarly journals Biosynthesis of Gold Nanoparticle using Cell-free Extract of Clinical Isolates Staphylococcus Aureus and Escherichia Coli

2019 ◽  
Vol 6 (2) ◽  
pp. 107-116
Author(s):  
Y. Valentina ◽  
Mohd Yousuf Rather ◽  
A. Yogamoorthi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Lattice Structure ◽  
Colour Change ◽  
Morphological Characteristics ◽  
Biological Synthesis ◽  
Cell Free Extract ◽  
E Coli

Present study reports on biological synthesis of gold nanoparticles using cell free extract of Staphylococcus aureus and Escherichia coli. The cold extract is allowed to react with both 1mM and 10 mM solutions of HAuCl4 which showed a colour change from yellow to dark cherry red after 1 hr. Gold nanoparticle formation is confirmed with UV-Visible spectrophotometer at 547nm.The 10mM concentration is found to be better for synthesis of more NPs using E. coli. XRD pattern exhibited 2θ values38.18o, 44.39o, 64.58o, 77.55o of 2θ value, corresponding to Bragg’s diffraction at 111, 200, 220 and 311 plane of lattice structure closely matching with the standard values given in ICDD reference file. SEM analysis revealed morphological characteristics of nanoparticles of different sizes ranging from 70 to 200 nm. Thus, the present study throws new light on the suitability of E. coli as an alternative for conventional methods of chemical synthesis of gold nanoparticles.  

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