scholarly journals Evaluation of Open Hollow Hydroxyapatite Microsphere on Bone Regeneration in Rat Calvarial Defects

2019 ◽  
Author(s):  
Youqu Shen ◽  
Mohamed Rahaman ◽  
Yongxian Liu ◽  
Yue-Wern Huang
Keyword(s):  
Surface Roughness ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Convex Surface ◽  
Total Surface Area ◽  
Calvarial Defect ◽  
New Bone Formation ◽  
Calvarial Defects ◽  
The Difference ◽  
Rat Calvarial Defect

AbstractHollow hydroxyapatite (HA) microspheres showed the ability to facilitate bone regeneration in rats with non-healing calvarial defects. However, new bone formation in the rat calvarial defect implanted with the closed HA microspheres was limited. The objective of this work is to evaluate size-, time, and structure-dependent bone regeneration between open and closed HA microspheres in an osseous model. Open HA microspheres were obtained by sectioning closed HA microspheres. The open HA microsphere had dense convex surface and rough and porous concave surface. For both size ranges (ϕ106-150 μm vs. ϕ212-250 μm), the open HA microsphere were more effective in facilitating bone regeneration than the closed HA microspheres in rat calvarial defects. Bone regeneration in the open HA microspheres (49 ± 7% for ϕ106-150 μm; 40 ± 8% for ϕ212-250 μm) were higher than the closed HA microsphere (26 ± 8% for ϕ106-150 μm; 30 ± 9% for ϕ212-250 μm) at 12 weeks. Furthermore, the open HA microspheres of smaller size showed a significant increase in bone regeneration than the open HA microspheres of larger size at both 6 weeks and 12 weeks. The difference in bone regeneration between these microspheres could be due to their differences in microstructures, namely curvature, concavity, porosity, surface roughness, and total surface area available for cells to attached to.

The Effects of Collagen Membrane Coated with PLGA on Bone Regeneration in Rat Calvarial Defects

2007 ◽  
Vol 342-343 ◽  
pp. 357-360
Author(s):  
Kun Young Song ◽  
Yoo Jung Um ◽  
Ui Won Jung ◽  
Yong Keun Lee ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Collagen Membrane ◽  
Histomorphometric Analysis ◽  
Local Bone ◽  
Calvarial Defects ◽  
Formation Behavior ◽  
Surgical Implantation ◽  
Collagen Membranes ◽  
Rat Calvarial Defect

The purpose of this study was to evaluate the effects of collagen membrane coated with PLGA on bone regeneration in rat calvarial defect. Five groups of 10 animals each received either collagen membrane coated with 0.5%, 1%, 3% concentration of PLGA, collagen membrane only or surgical control. Each group of animals was healed into 2 healing periods of 2(5 animals) and 8(5 animals)weeks and histologic and histomorphometric analysis were done. The results of the following study revealed that surgical implantation of collagen membranes coated with PLGA enhanced local bone formation at both 2 and 8 weeks independent of different PLGA concentrations. In conclusion, collagen membrane coated with PLGA shows a significant bone formation behavior irrespective of their concentration.

Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model

2017 ◽  
Vol 52 ◽  
pp. 277-286 ◽  
Author(s):  
Jae Yong Lee ◽  
Sung Eun Kim ◽  
Young-Pil Yun ◽  
Sung-Wook Choi ◽  
Daniel I. Jeon ◽  
...  
Keyword(s):  
Bone Formation ◽  
Plga Microspheres ◽  
Calvarial Defect ◽  
New Bone Formation ◽  
Defect Model ◽  
Rat Calvarial Defect

scholarly journals Effects of Polylactide Copolymer Implants and Platelet-Rich Plasma on Bone Regeneration within a Large Calvarial Defect in Sheep

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Bartłomiej Błaszczyk ◽  
Wojciech Kaspera ◽  
Krzysztof Ficek ◽  
Maciej Kajor ◽  
Marcin Binkowski ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Healing ◽  
Platelet Rich Plasma ◽  
Calvarial Defect ◽  
Proof Of Concept ◽  
Adult Sheep ◽  
The Future ◽  
Calvarial Defects

The aim of this study was to verify whether L-lactide/DL-lactide copolymer 80/20 (PLDLLA) and platelet-rich plasma (PRP) trigger bone formation within critical-sized calvarial defects in adult sheep (n=6). Two craniectomies, each ca. 3 cm in diameter, were created in each animal. The first craniectomy was protected with an inner polylactide membrane, filled with PRP-polylactide granules, and covered with outer polylactide membrane. The second control craniectomy was left untreated. The animals were euthanized at 6, 7, 17, 19, 33, and 34 weeks after surgery, and the quality and the rate of reossification were assessed histomorphometrically and microtomographically. The study demonstrated that application of implants made of PLDLLA 80/20 combined with an osteopromotive substance (e.g., PRP) may promote bone healing in large calvarial defect in sheep. These promising proof-of-concept studies need to be verified in the future on a larger cohort of animals and over a longer period of time in order to draw definitive conclusions.

scholarly journals In Vivo Bone Regeneration Induced by a Scaffold of Chitosan/Dicarboxylic Acid Seeded with Human Periodontal Ligament Cells

2019 ◽  
Vol 20 (19) ◽  
pp. 4883 ◽  
Author(s):  
Teerawat Sukpaita ◽  
Suwabun Chirachanchai ◽  
Pornchanok Suwattanachai ◽  
Vincent Everts ◽  
Atiphan Pimkhaokham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Formation ◽  
Dicarboxylic Acid ◽  
Bone Regeneration ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Micro Ct ◽  
New Bone Formation ◽  
Calvarial Defects

Chitosan/dicarboxylic acid (CS/DA) scaffold has been developed as a bone tissue engineering material. This study evaluated a CS/DA scaffold with and without seeded primary human periodontal ligament cells (hPDLCs) in its capacity to regenerate bone in calvarial defects of mice. The osteogenic differentiation of hPDLCs was analyzed by bone nodule formation and gene expression. In vivo bone regeneration was analyzed in mice calvarial defects. Eighteen mice were divided into 3 groups: one group with empty defects, one group with defects with CS/DA scaffold, and a group with defects with CS/DA scaffold and with hPDLCs. After 6 and 12 weeks, new bone formation was assessed using microcomputed tomography (Micro-CT) and histology. CS/DA scaffold significantly promoted in vitro osteoblast-related gene expression (RUNX2, OSX, COL1, ALP, and OPN) by hPDLCs. Micro-CT revealed that CS/DA scaffolds significantly promoted in vivo bone regeneration both after 6 and 12 weeks (p < 0.05). Histological examination confirmed these findings. New bone formation was observed in defects with CS/DA scaffold; being similar with and without hPDLCs. CS/DA scaffolds can be used as a bone regenerative material with good osteoinductive/osteoconductive properties.

Investigation of the effects of semaphorin 3A on new bone formation in a rat calvarial defect model

2019 ◽  
Vol 47 (3) ◽  
pp. 473-483 ◽  
Author(s):  
Sevinç Kenan ◽  
Özen Doğan Onur ◽  
Seyhun Solakoğlu ◽  
Tuğba Kotil ◽  
Mustafa Ramazanoğlu ◽  
...  
Keyword(s):  
Bone Formation ◽  
Calvarial Defect ◽  
Semaphorin 3A ◽  
New Bone Formation ◽  
Defect Model ◽  
Rat Calvarial Defect

scholarly journals Digital Customized Titanium Mesh for Bone Regeneration of Vertical, Horizontal and Combined Defects: A Case Series

Medicina ◽  
2021 ◽  
Vol 57 (1) ◽  
pp. 60
Author(s):  
Daniele De Santis ◽  
Federico Gelpi ◽  
Giuseppe Verlato ◽  
Umberto Luciano ◽  
Lorena Torroni ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Case Series ◽  
Guided Bone Regeneration ◽  
Point Of View ◽  
Significant Gain ◽  
Titanium Mesh ◽  
New Bone Formation ◽  
Correct Method ◽  
Combined Defects

Background and Objective: Guided bone regeneration allows new bone formation in anatomical sites showing defects preventing implant rehabilitation. Material and Methods: The present case series reported the outcomes of five patients treated with customized titanium meshes manufactured with a digital workflow for achieving bone regeneration at future implant sites. A significant gain in both width and thickness was achieved for all patients. Results: From a radiographic point of view (CBTC), satisfactory results were reached both in horizontal and vertical defects. An average horizontal gain of 3.6 ± 0.8 mm and a vertical gain of 5.2 ± 1.1 mm. Conclusions: The findings from this study suggest that customized titanium meshes represent a valid method to pursue guided bone regeneration in horizontal, vertical or combined defects. Particular attention must be paid by the surgeon in the packaging of the flap according to a correct method called the “poncho” technique in order to reduce the most frequent complication that is the exposure of the mesh even if a partial exposure of one mesh does not compromise the final outcome of both the reconstruction and the healing of the implants.

scholarly journals Bone Formation With the Combination of Simvastatin and Calcium Sulfate in Critical-Sized Rat Calvarial Defect

2007 ◽  
Vol 104 (4) ◽  
pp. 384-386 ◽  
Author(s):  
Myat Nyan ◽  
Daisuke Sato ◽  
Mitsumasa Oda ◽  
Tetsu Machida ◽  
Hiroshi Kobayashi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Calcium Sulfate ◽  
Calvarial Defect ◽  
Rat Calvarial Defect

Melatonin decorated 3D-printed beta-tricalcium phosphate scaffolds promoting bone regeneration in a rat calvarial defect model

2019 ◽  
Vol 7 (20) ◽  
pp. 3250-3259 ◽  
Author(s):  
Yali Miao ◽  
Yunhua Chen ◽  
Xiao Liu ◽  
Jingjing Diao ◽  
Naru Zhao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
3D Printed ◽  
Rat Calvarial Defect

3D-printed β-TCP scaffolds decorated with melatonin via dopamine mussel-inspired chemistry enhance the osteogenesis and in vivo bone regeneration.

scholarly journals Bone Regeneration Using a Three-Dimensional Hexahedron Channeled BCP Block Combined with Bone Morphogenic Protein-2 in Rat Calvarial Defects

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2435 ◽  
Author(s):  
So-Yeun Kim ◽  
Eun-Bin Bae ◽  
Jae-Woong Huh ◽  
Jong-Ju Ahn ◽  
Hyun-Young Bae ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Three Dimensional ◽  
Bone Morphogenetic Protein 2 ◽  
Graft Material ◽  
Implant Placement ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Calvarial Defects ◽  
Rat Calvarial Defect

It is important to obtain sufficient bone mass before implant placement on alveolar bone, and synthetic bone such as biphasic calcium phosphate (BCP) has been studied to secure this. This study used a BCP block bone with a specific structure of the three-dimensional (3D) hexahedron channel and coating with recombinant human bone morphogenetic protein-2 (rhBMP-2) impregnated carboxymethyl cellulose (CMC) was used to examine the enhancement of bone regeneration of this biomaterial in rat calvarial defect. After the preparation of critical-size calvarial defects in fifteen rats, defects were divided into three groups and were implanted with the assigned specimen (n = 5): Boneplant (untreated 3D hexahedron channeled BCP block), Boneplant/CMC (3D hexahedron channeled BCP block coated with CMC), and Boneplant/CMC/BMP (3D hexahedron channeled BCP block coated with CMC containing rhBMP-2). After 4 weeks, the volumetric, histologic, and histometric analyses were conducted to measure the newly formed bone. Histologically, defects in the Boneplant/CMC/BMP group were almost completely filled with new bone compared to the Boneplant and Boneplant/CMC groups. The new bone volume (P < 0.05) and area (P < 0.001) in the Boneplant/CMC/BMP group (20.12% ± 2.17, 33.79% ± 3.66) were much greater than those in the Boneplant (10.77% ± 4.8, 16.48% ± 9.11) and Boneplant/CMC (10.72% ± 3.29, 16.57% ± 8.94) groups, respectively. In conclusion, the 3D hexahedron channeled BCP block adapted rhBMP-2 with carrier CMC showed high possibility as an effective bone graft material.

scholarly journals Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

Materials ◽  
2019 ◽  
Vol 12 (10) ◽  
pp. 1613
Author(s):  
Eunhye Jang ◽  
Ja-Youn Lee ◽  
Eun-Young Lee ◽  
Hyun Seok
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Human Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Regeneration Effect ◽  
Recombinant Human Bone

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.

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