A chemiluminescence imaging array for the detection of cancer cells by dual-aptamer recognition and bio-bar-code nanoprobe-based rolling circle amplification

2013 ◽  
Vol 49 (33) ◽  
pp. 3452 ◽  
Author(s):  
Sai Bi ◽  
Bin Ji ◽  
Zhipeng Zhang ◽  
Shusheng Zhang
Keyword(s):  
Cancer Cells ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Bar Code ◽  
Imaging Array

scholarly journals A Label-Free Fluorescent Sensor Based on the Formation of Poly(thymine)-Templated Copper Nanoparticles for the Sensitive and Selective Detection of MicroRNA from Cancer Cells

Chemosensors ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 52
Author(s):  
Qian Ma ◽  
Zhiqiang Gao ◽  
Hiranya Dayal ◽  
Sam Fong Yau Li
Keyword(s):  
Cancer Cells ◽  
Copper Nanoparticles ◽  
Fluorescent Sensor ◽  
Rolling Circle Amplification ◽  
High Specificity ◽  
Excitation Wavelength ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Highly Sensitive

In this work, a simple and label-free fluorescence “off” to “on” platform was designed for the sensitive and selective detection of microRNA (miRNA) in cancer cells. This method utilized a padlock DNA-based rolling circle amplification (P-RCA) to synthesize fluorescent poly(thymine) (PolyT) which acted as a template for the synthesis of copper nanoparticles (CuNPs) within 10 minutes under mild conditions. While the repeated PolyT sequence was used as the template for CuNP synthesis, other non-PolyT parts (single strand-DNAs without the capacity to act as the template for CuNP formation) served as “smart glues” or rigid linkers to build complex nanostructures. Under the excitation wavelength of 340 nm, the synthesized CuNPs emitted strong red fluorescence effectively at 620 nm. To demonstrate the use of this method as a universal biosensor platform, lethal-7a (let-7a) miRNA was chosen as the standard target. This sensor could achieve highly sensitive and selective detection of miRNA in the presence of other homologous analogues for the combination of P-RCA with the fluorescent copper nanoparticle. Overall, this novel label-free method holds great potential in the sensitive detection of miRNA with high specificity in real samples.

scholarly journals Expanding the Chemical Repertoire of DNA Nanomaterials Generated by Rolling Circle Amplification

2020 ◽  
Author(s):  
Ysobel Baker ◽  
Liyiwen Yuan ◽  
jinfeng Chen ◽  
Roman Belle ◽  
Robert Carlisle ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Cells ◽  
Hybrid Materials ◽  
High Performance ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Magnesium Pyrophosphate ◽  
Protein Immobilisation ◽  
Diagnostic Potential

<p>Rolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging, protein immobilisation, and drug delivery, yet limited chemistry is available to expand their functionality. Here, we present an orthogonal strategy to produce densely modified RCA products and DNFs. We show that it is possible to selectively functionalise the DNA component of these materials, their protein cargo, or both, thereby greatly expanding the chemical repertoire available to these systems. We then use this methodology to construct DNFs bearing multiple surface aptamers capable of binding to cancer cells that overexpress the HER2 oncobiomarker, demonstrating the therapeutic and diagnostic potential of this chemistry.</p>

Ultrasensitive electrochemical detection of breast cancer cells based on DNA-rolling-circle-amplification-directed enzyme-catalyzed polymerization

2015 ◽  
Vol 51 (11) ◽  
pp. 2114-2117 ◽  
Author(s):  
Qinglin Sheng ◽  
Ni Cheng ◽  
Wushuang Bai ◽  
Jianbin Zheng
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Electrochemical Detection ◽  
Breast Cancer Cells ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Catalyzed Polymerization

An ultrasensitive cytosensor based on DNA-rolling-circle-amplification-directed enzyme-catalyzed polymerization is demonstrated.

scholarly journals Expanding the Chemical Repertoire of DNA Nanomaterials Generated by Rolling Circle Amplification

2020 ◽  
Author(s):  
Ysobel Baker ◽  
Liyiwen Yuan ◽  
jinfeng Chen ◽  
Roman Belle ◽  
Robert Carlisle ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Cells ◽  
Hybrid Materials ◽  
High Performance ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Magnesium Pyrophosphate ◽  
Protein Immobilisation ◽  
Diagnostic Potential

<p>Rolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging, protein immobilisation, and drug delivery, yet limited chemistry is available to expand their functionality. Here, we present an orthogonal strategy to produce densely modified RCA products and DNFs. We show that it is possible to selectively functionalise the DNA component of these materials, their protein cargo, or both, thereby greatly expanding the chemical repertoire available to these systems. We then use this methodology to construct DNFs bearing multiple surface aptamers capable of binding to cancer cells that overexpress the HER2 oncobiomarker, demonstrating the therapeutic and diagnostic potential of this chemistry.</p>

Fluorescence biosensor for folate receptors in cancer cells based on terminal protection and hyperbranched rolling circle amplification

2016 ◽  
Vol 8 (32) ◽  
pp. 6231-6235 ◽  
Author(s):  
Dawei Li ◽  
Yuanzheng Ma ◽  
Ying Zhang ◽  
Zhenyu Lin
Keyword(s):  
Cancer Cells ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Fluorescence Sensor ◽  
Folate Receptors ◽  
Rolling Circle ◽  
Fluorescence Biosensor

A fluorescence sensor for folate receptors based on terminal protection and hyperbranched rolling circle amplification with high sensitivity and specificity has been developed.

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

Sign in / Sign up

Export Citation Format

Share Document