scholarly journals Biodegradable PEI modified complex micelles as gene carriers with tunable gene transfection efficiency for ECs

2016 ◽  
Vol 4 (5) ◽  
pp. 997-1008 ◽  
Author(s):  
Juan Lv ◽  
Jing Yang ◽  
Xuefang Hao ◽  
Xiangkui Ren ◽  
Yakai Feng ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Vascular Grafts ◽  
Gene Carriers ◽  
Coronary Diseases ◽  
Potential Strategy ◽  
Complex Micelles

In recent years, gene therapy has evoked an increasing interest in clinical treatments of coronary diseases because it is a potential strategy to realize rapid endothelialization of artificial vascular grafts.

PGMA-based gene carriers with lipid molecules

2016 ◽  
Vol 4 (8) ◽  
pp. 1233-1243 ◽  
Author(s):  
Chen Xu ◽  
Bingran Yu ◽  
Hao Hu ◽  
Muhammad Naeem Nizam ◽  
Wei Yuan ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Tumor Treatment ◽  
Gene Carriers ◽  
High Gene ◽  
Low Cytotoxicity

A series of effective cationic conjugations of lipid molecules with low cytotoxicity and high gene transfection efficiency were readily designed for gene therapy and tumor treatment.

Non-Viral Vectors for Gene Delivery

2018 ◽  
Vol 9 (1) ◽  
pp. 4-11 ◽  
Author(s):  
Aparna Bansal ◽  
Himanshu
Keyword(s):  
Gene Therapy ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Expression System ◽  
Target Cells ◽  
Specific Expression ◽  
Naked Dna

Introduction: Gene therapy has emerged out as a promising therapeutic pave for the treatment of genetic and acquired diseases. Gene transfection into target cells using naked DNA is a simple and safe approach which has been further improved by combining vectors or gene carriers. Both viral and non-viral approaches have achieved a milestone to establish this technique, but non-viral approaches have attained a significant attention because of their favourable properties like less immunotoxicity and biosafety, easy to produce with versatile surface modifications, etc. Literature is rich in evidences which revealed that undoubtedly, non–viral vectors have acquired a unique place in gene therapy but still there are number of challenges which are to be overcome to increase their effectiveness and prove them ideal gene vectors. Conclusion: To date, tissue specific expression, long lasting gene expression system, enhanced gene transfection efficiency has been achieved with improvement in delivery methods using non-viral vectors. This review mainly summarizes the various physical and chemical methods for gene transfer in vitro and in vivo.

Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles

2021 ◽  
Vol 9 (7) ◽  
pp. 2454-2466
Author(s):  
Yingying Liu ◽  
Yuli Zhou ◽  
Jinfeng Xu ◽  
Hui Luo ◽  
Yao Zhu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Molecular Imaging ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Tumor Gene Therapy ◽  
Ultrasound Molecular Imaging ◽  
Tumor Gene

A novel dual-targeted cationic microbubbles help to improve gene transfection efficiency.

Regulation of gene transfection by cell size, shape and elongation on micropatterned surfaces

2021 ◽  
Author(s):  
Yongtao Wang ◽  
Yingjun Yang ◽  
Toru Yoshitomi ◽  
Naoki Kawazoe ◽  
Yingnan Yang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cell Size ◽  
Tissue Repair ◽  
Gene Transfection ◽  
P Gene ◽  
Gene Carriers ◽  
Potential Applications

Gene transfection has been widely studied due to its potential applications in tissue repair and gene therapy. Many studies have focused on designing gene carriers and developing novel transfection techniques....

scholarly journals Gene Transfection of Human Turbinate Mesenchymal Stromal Cells Derived from Human Inferior Turbinate Tissues

2016 ◽  
Vol 2016 ◽  
pp. 1-10
Author(s):  
Jin Seon Kwon ◽  
Seung Hun Park ◽  
Ji Hye Baek ◽  
Truong Minh Dung ◽  
Sung Won Kim ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
High Efficiency ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Inferior Turbinate ◽  
Nonviral Gene Delivery ◽  
Charge Ratio ◽  
Pei Nanoparticles

Human turbinate mesenchymal stromal cells (hTMSCs) are novel stem cells derived from nasal inferior turbinate tissues. They are easy to isolate from the donated tissue after turbinectomy or conchotomy. In this study, we applied hTMSCs to a nonviral gene delivery system using polyethyleneimine (PEI) as a gene carrier; furthermore, the cytotoxicity and transfection efficiency of hTMSCs were evaluated to confirm their potential as resources in gene therapy. DNA-PEI nanoparticles (NPs) were generated by adding the PEI solution to DNA and were characterized by a gel electrophoresis and by measuring particle size and surface charge of NPs. The hTMSCs were treated with DNA-PEI NPs for 4 h, and toxicity of NPs to hTMSCs and gene transfection efficiency were monitored using MTT assay, fluorescence images, and flow cytometry after 24 h and 48 h. At a high negative-to-positive charge ratio, DNA-PEI NPs treatment led to cytotoxicity of hTMSCs, but the transfection efficiency of DNA was increased due to the electrostatic effect between the NPs and the membranes of hTMSCs. Importantly, the results of this research verified that PEI could deliver DNA into hTMSCs with high efficiency, suggesting that hTMSCs could be considered as untapped resources for applications in gene therapy.

scholarly journals Fabrication and Biological Activities of Plasmid DNA Gene Carrier Nanoparticles Based on Biodegradable l-Tyrosine Polyurethane

2021 ◽  
Vol 15 (1) ◽  
pp. 17
Author(s):  
Soo-Yong Park ◽  
Yang H. Yun ◽  
Bum-Joon Park ◽  
Hyung-Il Seo ◽  
Ildoo Chung
Keyword(s):  
Gene Therapy ◽  
Cellular Uptake ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Biological Activities ◽  
Average Diameter ◽  
Gene Carrier ◽  
Suitable Alternative ◽  
High Transfection Efficiency ◽  
Gene Carriers

Gene therapy is a suitable alternative to chemotherapy due to the complications of drug resistance and toxicity of drugs, and is also known to reduce the occurrence of cellular mutation through the use of gene carriers. In this study, gene carrier nanoparticles with minimal toxicity and high transfection efficiency were fabricated from a biocompatible and biodegradable polymer, l-tyrosine polyurethane (LTU), which was polymerized from presynthesized desaminotyrosyl tyrosine hexyl ester (DTH) and polyethylene glycol (PEG), by using double emulsion and solvent evaporation techniques, resulting in the formation of porous nanoparticles, and then used to evaluate their potential biological activities through molecular controlled release and transfection studies. To assess cellular uptake and transfection efficiency, two model drugs, fluorescently labeled bovine serum albumin (FITC-BSA) and plasmid DNA-linear polyethylenimine (LPEI) complex, were successfully encapsulated in nanoparticles, and their transfection properties and cytotoxicities were evaluated in LX2 as a normal cell and in HepG2 and MCF7 as cancer cells. The morphology and average diameter of the LTU nanoparticles were confirmed using light microscopy, transmission electron microscopy, and dynamic light scattering, while confocal microscopy was used to validate the cellular uptake of FITC-BSA-encapsulated LTU nanoparticles. Moreover, the successful cellular uptake of LTU nanoparticles encapsulated with pDNA-LPEI and the high transfection efficiency, confirmed by gel electrophoresis and X-gal assay transfection, indicated that LTU nanoparticles had excellent cell adsorption ability, facilitated gene encapsulation, and showed the sustained release tendency of genes through transfection experiments, with an optimal concentration ratio of pDNA and LPEI of 1:10. All the above characteristics are ideal for gene carriers designed to transport and release drugs into the cytoplasm, thus facilitating effective gene therapy.

DEGRADABLE POLYETHYLENIMINE DERIVATIVES AS GENE CARRIERS

Nano LIFE ◽  
2012 ◽  
Vol 02 (01) ◽  
pp. 1230004 ◽  
Author(s):  
YOU-KYOUNG KIM ◽  
QUYNH-PHUONG LUU ◽  
MOHAMMAD ARIFUL ISLAM ◽  
YUN-JAIE CHOI ◽  
CHONG-SU CHO ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Viral Vectors ◽  
Large Scale ◽  
Transfection Efficiency ◽  
Endosomal Escape ◽  
Scale Production ◽  
Nonviral Vectors ◽  
High Transfection Efficiency ◽  
Gene Carriers

Gene therapy is a treatment for inborn and acquired diseases, although the development of safe and effective gene delivery system is a great challenge to make a gene therapy a success. Viral vectors have been used in a majority of clinics because of their high transfection efficiency in vitro and in vivo. However, their use has been limited because of several drawbacks, such as induction of immune response, recombination of wild-type viruses, limitation in the size of inserted gene, and difficulty in large-scale production. Nonviral vectors have been widely proposed safe alternatives to viral vectors because they have low immunogenicity, flexibility in the size of gene to be delivered, cell targetibility, and easy scalability of production, although they have low transfection efficiency compared to viral vectors. Among nonviral vectors, polyethylenimine (PEI) has been widely used as a standard gene carriers due to its high pH-buffering capacity for endosomal escape although high-molecular-weight PEI is too toxic owing to non-degradability. Recently, many types of degradable PEI have been studied due to high transfection efficiency with lower cytotoxicity. This review explains recent progress on the development of degradable PEIs as nonviral vectors. The present paper summarizes the transfection efficiency of DNA or silencing efficiency of small interfering RNA (siRNA) based on the kinds of degradable linkage between low PEI and crosslinkers. Degradable linkages, such as ester, disulfide, imines, carbamate, amide and ketal in the degradable PEIs are covered.

scholarly journals Injectable “nano-micron” combined gene-hydrogel microspheres for local treatment of osteoarthritis

2022 ◽  
Vol 14 (1) ◽  
Author(s):  
Bin Li ◽  
Fei Wang ◽  
Fangqiong Hu ◽  
Tao Ding ◽  
Ping Huang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Articular Cartilage ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Direct Injection ◽  
Local Treatment ◽  
Type Ii Collagen ◽  
Transfection Efficacy ◽  
Hydrogel Microspheres

AbstractSustained and controllable local gene therapy is a potential method for treating osteoarthritis (OA) through the delivery of therapeutic microRNAs (miRNAs) to targeted cells. However, direct injection of crude miRNAs for local gene therapy is limited due to its inadequate transfection efficiency, easy inactivation, and short half-life. Here, a multifunctional gene vector, arginine, histidine, and phenylalanine-modified generation 5 polyamidoamine (named G5-AHP), was employed to form G5-AHP/miR-140 nanoparticles by forming a complex with microRNA-140 (miR-140). Then, the nanoparticles were entrapped in hydrogel microspheres (MSs) to construct a “nano-micron” combined gene hydrogel to alleviate the degradation of articular cartilage. Monodisperse gelatin methacryloyl hydrogel MSs were produced under ultraviolet light using one-step innovative microfluidic technology. Evenly dispersed MSs showed better injectability in sustainable and matrix metalloproteinases (MMPs)-responsive degradation methods for local gene delivery. The G5-AHP/miR-140 nanoparticles released from the MSs exhibited high gene transfection efficacy and long-term bioactivity, facilitated endocytosis, and thus maintained the metabolic balance of cartilage matrix by promoting the expression of type II collagen and inhibiting the expression of a disintegrin and metalloproteinase with thrombospondin motifs-5 and MMP13 in chondrocytes. After injection of the “nano-micron” combined gene hydrogel into the articular cavity of the OA model, the gene hydrogel increased G5-AHP/miR-140 nanoparticle retention, prevented articular cartilage degeneration, and reduced osteophyte formation in a surgically induced mouse model of OA. The present study provides a novel cell-free approach to alleviate the progression of OA that shows potential for locally injected gene delivery systems.

Zn-promoted gene transfection efficiency for non-viral vectors: a mechanism study

2021 ◽  
Author(s):  
Xiao-Qi Yu ◽  
Rui-Mo Zhao ◽  
Yu Guo ◽  
Hui-Zhen Yang ◽  
Ji Zhang
Keyword(s):  
Gene Therapy ◽  
Nucleic Acid ◽  
Gene Delivery ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Viral Gene ◽  
Mechanism Study ◽  
High Affinity ◽  
Gene Vectors

The development of cationic non-viral gene vectors that may overcome the obstacles in gene delivery is of great significance to gene therapy. Metallic complexes with high affinity to nucleic acid...

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