scholarly journals Gene Transfection of Human Turbinate Mesenchymal Stromal Cells Derived from Human Inferior Turbinate Tissues

2016 ◽  
Vol 2016 ◽  
pp. 1-10
Author(s):  
Jin Seon Kwon ◽  
Seung Hun Park ◽  
Ji Hye Baek ◽  
Truong Minh Dung ◽  
Sung Won Kim ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
High Efficiency ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Inferior Turbinate ◽  
Nonviral Gene Delivery ◽  
Charge Ratio ◽  
Pei Nanoparticles

Human turbinate mesenchymal stromal cells (hTMSCs) are novel stem cells derived from nasal inferior turbinate tissues. They are easy to isolate from the donated tissue after turbinectomy or conchotomy. In this study, we applied hTMSCs to a nonviral gene delivery system using polyethyleneimine (PEI) as a gene carrier; furthermore, the cytotoxicity and transfection efficiency of hTMSCs were evaluated to confirm their potential as resources in gene therapy. DNA-PEI nanoparticles (NPs) were generated by adding the PEI solution to DNA and were characterized by a gel electrophoresis and by measuring particle size and surface charge of NPs. The hTMSCs were treated with DNA-PEI NPs for 4 h, and toxicity of NPs to hTMSCs and gene transfection efficiency were monitored using MTT assay, fluorescence images, and flow cytometry after 24 h and 48 h. At a high negative-to-positive charge ratio, DNA-PEI NPs treatment led to cytotoxicity of hTMSCs, but the transfection efficiency of DNA was increased due to the electrostatic effect between the NPs and the membranes of hTMSCs. Importantly, the results of this research verified that PEI could deliver DNA into hTMSCs with high efficiency, suggesting that hTMSCs could be considered as untapped resources for applications in gene therapy.

Non-Viral Vectors for Gene Delivery

2018 ◽  
Vol 9 (1) ◽  
pp. 4-11 ◽  
Author(s):  
Aparna Bansal ◽  
Himanshu
Keyword(s):  
Gene Therapy ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Expression System ◽  
Target Cells ◽  
Specific Expression ◽  
Naked Dna

Introduction: Gene therapy has emerged out as a promising therapeutic pave for the treatment of genetic and acquired diseases. Gene transfection into target cells using naked DNA is a simple and safe approach which has been further improved by combining vectors or gene carriers. Both viral and non-viral approaches have achieved a milestone to establish this technique, but non-viral approaches have attained a significant attention because of their favourable properties like less immunotoxicity and biosafety, easy to produce with versatile surface modifications, etc. Literature is rich in evidences which revealed that undoubtedly, non–viral vectors have acquired a unique place in gene therapy but still there are number of challenges which are to be overcome to increase their effectiveness and prove them ideal gene vectors. Conclusion: To date, tissue specific expression, long lasting gene expression system, enhanced gene transfection efficiency has been achieved with improvement in delivery methods using non-viral vectors. This review mainly summarizes the various physical and chemical methods for gene transfer in vitro and in vivo.

Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles

2021 ◽  
Vol 9 (7) ◽  
pp. 2454-2466
Author(s):  
Yingying Liu ◽  
Yuli Zhou ◽  
Jinfeng Xu ◽  
Hui Luo ◽  
Yao Zhu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Molecular Imaging ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Tumor Gene Therapy ◽  
Ultrasound Molecular Imaging ◽  
Tumor Gene

A novel dual-targeted cationic microbubbles help to improve gene transfection efficiency.

scholarly journals Nonviral gene delivery of erythropoietin by mesenchymal stromal cells

Gene Therapy ◽  
2011 ◽  
Vol 19 (5) ◽  
pp. 550-560 ◽  
Author(s):  
F Scheibe ◽  
N Gladow ◽  
P Mergenthaler ◽  
A H Tucker ◽  
A Meisel ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Nonviral Gene Delivery

scholarly journals Human Mesenchymal Stromal Cells Unveil an Unexpected Differentiation Potential toward the Dopaminergic Neuronal Lineage

2020 ◽  
Vol 21 (18) ◽  
pp. 6589
Author(s):  
Giulia Gaggi ◽  
Andrea Di Credico ◽  
Pascal Izzicupo ◽  
Francesco Alviano ◽  
Michele Di Mauro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Dopaminergic Neurons ◽  
High Efficiency ◽  
Ethical Issues ◽  
Differentiation Potential ◽  
Homogeneous Population ◽  
Human Mesenchymal Stromal Cells ◽  
Midbrain Dopaminergic Neurons

Degeneration of dopaminergic neurons represents the cause of many neurodegenerative diseases, with increasing incidence worldwide. The replacement of dead cells with new healthy ones may represent an appealing therapeutic approach to these pathologies, but currently, only pluripotent stem cells can generate dopaminergic neurons with high efficiency. However, with the use of these cells arises safety and/or ethical issues. Human mesenchymal stromal cells (hFM-MSCs) are perinatal stem cells that can be easily isolated from the amniochorionic membrane after delivery. Generally considered multipotent, their real differentiative potential is not completely elucidated. The aim of this study was to analyze their stemness characteristics and to evaluate whether they may overcome their mesenchymal fate, generating dopaminergic neurons. We demonstrated that hFM-MSCs expressed embryonal genes OCT4, NANOG, SOX2, KLF4, OVOL1, and ESG1, suggesting they have some features of pluripotency. Moreover, hFM-MSCs that underwent a dopaminergic differentiation protocol gradually increased the transcription of dopaminergic markers LMX1b, NURR1, PITX3, and DAT. We finally obtained a homogeneous population of cells resembling the morphology of primary midbrain dopaminergic neurons that expressed the functional dopaminergic markers TH, DAT, and Nurr1. In conclusion, our results suggested that hFM-MSCs retain the expression of pluripotency genes and are able to differentiate not only into mesodermal cells, but also into neuroectodermal dopaminergic neuron-like cells.

Efficient plasmid-mediated gene transfection of ovine bone marrow mesenchymal stromal cells

Cytotherapy ◽  
2013 ◽  
Vol 15 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Paola Locatelli ◽  
Fernanda Daniela Olea ◽  
Anna Hnatiuk ◽  
Diana Sepúlveda ◽  
Juan Manuel Pérez Sáez ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Gene Transfection

Biodegradable nano-organosilica gene carrier for high-efficiency gene transfection

2020 ◽  
Vol 8 (12) ◽  
pp. 2483-2494
Author(s):  
Kun Zeng ◽  
Li Ma ◽  
Wenxiu Yang ◽  
Shan Lei ◽  
Mozhen Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Gene Vector ◽  
High Transfection Efficiency ◽  
In Cells

Guanidinated-fluorinated α-polylysine-modified organosilica nanoparticles can form a novel raisin-bread-like gene vector, which is disintegrated in cells by GSH to show high transfection efficiency.

scholarly journals The Development of Functional Non-Viral Vectors for Gene Delivery

2019 ◽  
Vol 20 (21) ◽  
pp. 5491 ◽  
Author(s):  
Patil ◽  
Gao ◽  
Lin ◽  
Li ◽  
Dang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Targeted Delivery ◽  
Viral Vectors ◽  
High Efficiency ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Gene Vectors ◽  
Potential Applications ◽  
Low Toxicity ◽  
Low Cytotoxicity

Gene therapy is manipulation in/of gene expression in specific cells/tissue to treat diseases. This manipulation is carried out by introducing exogenous nucleic acids, such as DNA or RNA, into the cell. Because of their negative charge and considerable larger size, the delivery of these molecules, in general, should be mediated by gene vectors. Non-viral vectors, as promising delivery systems, have received considerable attention due to their low cytotoxicity and non-immunogenicity. As research continued, more and more functional non-viral vectors have emerged. They not only have the ability to deliver a gene into the cells but also have other functions, such as the performance of fluorescence imaging, which aids in monitoring their progress, targeted delivery, and biodegradation. Recently, many reviews related to non-viral vectors, such as polymers and cationic lipids, have been reported. However, there are few reviews regarding functional non-viral vectors. This review summarizes the common functional non-viral vectors developed in the last ten years and their potential applications in the future. The transfection efficiency and the transport mechanism of these materials were also discussed in detail. We hope that this review can help researchers design more new high-efficiency and low-toxicity multifunctional non-viral vectors, and further accelerate the progress of gene therapy.

Mesenchymal Stromal Cells Genetically Engineered To Overexpress Insulin-Like Growth Factor-1 Provide Paracrine Support for Cell-Based Erythropoietin Therapy of Chronic Renal Failure-Induced Anemia.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2598-2598
Author(s):  
Terrence Kucic ◽  
Ian B. Copland ◽  
Jessica Cuerquis ◽  
Daniel L. Coutu ◽  
Lorraine E. Chalifour ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Renal Failure ◽  
Chronic Renal Failure ◽  
Growth Factor ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Mapk Pathway ◽  
Insulin Like Growth Factor ◽  
Igf I

Abstract Mesenchymal stromal cells (MSC) are a population of non-hematopoietic progenitors native to the bone marrow that are amenable to genetic engineering, making them attractive delivery vehicles for the in vivo production of therapeutic proteins, such as erythropoietin (Epo). We have previously demonstrated that MSC engineered to secrete Epo can be used for the long-term correction of renal failure-induced anemia [Eliopoulos et al., J Am Soc Nephrol. June 2006]. However, limited long-term transplanted cell survival compromises the efficacy of MSC-based gene therapy approaches. The current study provides evidence that co-implantation of MSC overexpressing Insulin-like growth factor-1 (IGF-I) improves MSC-based gene therapy of anemia by providing paracrine support to Epo-secreting MSC within a synthetic subcutaneous organoid. The IGF-I receptor was found to be expressed in murine MSC by RT-PCR, and protein expression was confirmed by immunoblot. We also demonstrated MSC MAPK pathway responsiveness to IGF-I stimulation in vitro and subsequent improvement of MSC survival following staurosporin-induced apoptosis. Murine MSC were transduced to overexpress either Epo or IGF-I (hereafter MSC-Epo and MSC-IGF) using retroviral vectors. MSC-Epo were subsequently admixed in a collagen matrix and implanted by subcutaneous injection in both naïve mice and a murine model of chronic renal failure, in combination with either MSC-IGF or null MSC. Mice receiving MSC-Epo in conjunction with MSC-IGF experienced a greater and significantly sustained elevation in hematocrit compared to control mice. In addition, mice co-implanted with MSC-IGF and MSC-Epo demonstrated a significant improvement in cardiac function compared to controls. In conclusion, cell-based gene therapy using co-implanted MSC-IGF represents a promising new strategy for the treatment of renal failure-induced anemia, as well as for the improvement of gene-enhanced MSC survival within implanted matrices.

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