Multiplex lateral flow immunoassay for five antibiotics detection based on gold nanoparticle aggregations

RSC Advances ◽  
2016 ◽  
Vol 6 (10) ◽  
pp. 7798-7805 ◽  
Author(s):  
Juan Peng ◽  
Yongwei Wang ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Aike Li ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Lateral Flow Immunoassay ◽  
Antibiotics Detection

A new immunochromatographic assay was developed for the simultaneous screening of five antibiotics that can coexist in milk, namely lincomycin, gentamicin, kanamycin, streptomycin, and neomycin, using five corresponding monoclonal antibodies.

A remarkable sensitivity enhancement in a gold nanoparticle-based lateral flow immunoassay for the detection of Escherichia coli O157:H7

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 45092-45097 ◽  
Author(s):  
Xi Cui ◽  
Youju Huang ◽  
Jingyun Wang ◽  
Lei Zhang ◽  
Yun Rong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticle ◽  
Quantitative Data ◽  
Visual Inspection ◽  
Sensitivity Enhancement ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157

The size and uniformity of AuNPs were optimized to maximally amplify both visual inspection signals and quantitative data of LFA.

scholarly journals Lateral Flow Immunoassay with the Signal Enhanced by Gold Nanoparticle Aggregates Based on Polyamidoamine Dendrimer

2013 ◽  
Vol 29 (8) ◽  
pp. 799-804 ◽  
Author(s):  
Guangyu SHEN ◽  
Hui XU ◽  
Anant S. GURUNG ◽  
Yunhui YANG ◽  
Guodong LIU
Keyword(s):  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Polyamidoamine Dendrimer ◽  
Nanoparticle Aggregates

scholarly journals Gold nanoparticle-based enhanced lateral flow immunoassay for detection of Cronobacter sakazakii in powdered infant formula

2018 ◽  
Vol 101 (5) ◽  
pp. 3835-3843 ◽  
Author(s):  
Ruili Pan ◽  
Yujun Jiang ◽  
Luhong Sun ◽  
Rui Wang ◽  
Kejin Zhuang ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Infant Formula ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Powdered Infant Formula ◽  
Cronobacter Sakazakii

scholarly journals Comparison of Nanosized Markers in Lateral Flow Immunoassay of Antibiotic Lincomycin

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 41
Author(s):  
Olga D. Hendrickson ◽  
Kseniya V. Serebrennikova ◽  
Elena A. Zvereva ◽  
Demid S. Popravko ◽  
Anatoly V. Zherdev ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Site Testing ◽  
Limits Of Detection ◽  
Chemical Contaminants

Improving the sensitivity of the competitive lateral flow immunoassay (LFIA) is important, given the increasing demands for the monitoring of chemical contaminants in food. The choice of nanosized marker is an essential task for improving the LFIA sensitivity. In this study, a CdSe/ZnS quantum dot (QD)-based LFIA combined with a portable reader was developed for rapid and quantitative detection of an antibiotic lincomycin (LIN). The performance of the proposed fluorescence LFIA was compared to the conventional gold nanoparticle (AuNP)-based LFIA realized with the same immunoreagents. The visual cutoff values were 10 ng/mL for AuNP-based LFIA and 20 ng/mL for QD-based LFIA. Furthermore, the instrumental limits of detection have been shown to be comparable for both nanosized markers and amounted to 0.4 ng/mL for AuNPs and 0.2 ng/mL for QDs, respectively. According to the results obtained, both LFIAs may be used for rapid, cost-effective, on-site testing of antibiotics, in particular LIN. However, the QD-based LFIA exhibits lowest limit of detection with the least immunoreagent consumption, which makes it economically beneficial.

scholarly journals COVALENT CONJUGATION OF ANTIBODY AND GOLD NANOPARTICLE FOR DEVELOPMENT OF LATERAL FLOW IMMUNOASSAY TEST STRIP

2018 ◽  
Vol 54 (4A) ◽  
pp. 323
Author(s):  
Truong Quoc Phong
Keyword(s):  
Gold Nanoparticle ◽  
Room Temperature ◽  
Limit Of Detection ◽  
Coupling Reaction ◽  
Test Strip ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Research Fields ◽  
Antibody Conjugates ◽  
Antigen Antibody

Nanotechnology is one of the fastest growing technologies in this era. Gold nanoparticle (AuNP) based immunoassays have been performed on the basis of antigen-antibody interaction using AuNP antibody conjugates. Lateral flow immunoassays(LFA) which are also based on AuNP antibody conjugates are useful innovation in nanotechnology and widely applied in medicine and research fields. However, there are some limitations of the present LFA kits such as sensitivity and stability. In the study, we showed the result of covalent conjugation of anti-rotavirus antibody and AuNP for generating a lateral flow immunoassay strip to detect rotavirus in fecal samples. The suitable conditions for coating polyethylene glycol (PEG) on the surface of AuNP were 10.0 mM PEG for 3 hours at room temperature (25 oC). Optimized conditions for covalent conjugation of antibody and AuNP were pH 4.0, 0.1 mg antibody/conjugate, 0.01 mM reactant EDC/NHS [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/(N-hydroxy sulfosuccinimide]. The coupling reaction was carried out at room temperature for 90 min. The conjugate pad, antibody immobilized nitrocellulose membrane strip were created with investigated conditions for generating an LFA test strip. The limit of detection of LFA test strip was determined by 1.6 × 105 virus particles/ml, three times lower than that of Rotaclone kit (UK). The generated strip could be used to detect rotavirus in fecal sample of patient. 

Detection of triclabendazole and three metabolites in bovine muscle samples with a gold nanoparticle-based lateral flow immunoassay

2019 ◽  
Vol 11 (42) ◽  
pp. 5478-5486 ◽  
Author(s):  
Zhongxing Wang ◽  
Xiaoling Wu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
Chuanlai Xu
Keyword(s):  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Lateral Flow Immunoassay ◽  
Bovine Muscle ◽  
Highly Sensitive

A highly sensitive mAb against TCD was produced by using a novel hapten, and basing on this mAb, a lateral flow immunoassay was developed for the rapid screening of TCD and its metabolites in foodstuff.

Google Analytics and quick response for advancement of gold nanoparticle-based dual lateral flow immunoassay for malaria – Plasmodium lactate dehydrogenase (pLDH)

2017 ◽  
Vol 9 (41) ◽  
pp. 5943-5951 ◽  
Author(s):  
Christian L. Mthembu ◽  
Myalowenkosi I. Sabela ◽  
Mbuso Mlambo ◽  
Lawrence M. Madikizela ◽  
Suvardhan Kanchi ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Gold Nanoparticle ◽  
Healthcare Workers ◽  
Malaria Diagnosis ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quick Response ◽  
Community Healthcare ◽  
Community Healthcare Workers

An assay with quick response barcodes improves malaria diagnosis, and enhances systems transferring data among community healthcare workers.

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