A remarkable sensitivity enhancement in a gold nanoparticle-based lateral flow immunoassay for the detection of Escherichia coli O157:H7

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 45092-45097 ◽  
Author(s):  
Xi Cui ◽  
Youju Huang ◽  
Jingyun Wang ◽  
Lei Zhang ◽  
Yun Rong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticle ◽  
Quantitative Data ◽  
Visual Inspection ◽  
Sensitivity Enhancement ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157

The size and uniformity of AuNPs were optimized to maximally amplify both visual inspection signals and quantitative data of LFA.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

scholarly journals Lateral flow immunoassay integrated with competitive and sandwich models for the detection of aflatoxin M1 and Escherichia coli O157:H7 in milk

2018 ◽  
Vol 101 (10) ◽  
pp. 8767-8777 ◽  
Author(s):  
Chun Wang ◽  
Juan Peng ◽  
Dao-Feng Liu ◽  
Ke-Yu Xing ◽  
Gang-Gang Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lateral Flow ◽  
Aflatoxin M1 ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157

Functional nanozyme mediated multi-readout and label-free lateral flow immunoassay for rapid detection of Escherichia coli O157:H7

2020 ◽  
Vol 329 ◽  
pp. 127224
Author(s):  
Zonghan Wang ◽  
Xiaolin Yao ◽  
Yongzhi Zhang ◽  
Rong Wang ◽  
Yanwei Ji ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Label Free

scholarly journals Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay

Sensors ◽  
2017 ◽  
Vol 17 (4) ◽  
pp. 753 ◽  
Author(s):  
Song Cheng ◽  
Ming-hui Chen ◽  
Gang-gang Zhang ◽  
Zhi-biao Yu ◽  
Dao-feng Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Accurate Detection ◽  
Ground Pork
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