scholarly journals Dual sensitivity enhancement in gold nanoparticle‐based lateral flow immunoassay for visual detection of carcinoembryonic antigen

2020 ◽  
Vol 1 (3) ◽  
pp. 161-172 ◽  
Author(s):  
Tohid Mahmoudi ◽  
Behnaz Shirdel ◽  
Behzad Mansoori ◽  
Behzad Baradaran
Keyword(s):  
Carcinoembryonic Antigen ◽  
Gold Nanoparticle ◽  
Visual Detection ◽  
Sensitivity Enhancement ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

A remarkable sensitivity enhancement in a gold nanoparticle-based lateral flow immunoassay for the detection of Escherichia coli O157:H7

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 45092-45097 ◽  
Author(s):  
Xi Cui ◽  
Youju Huang ◽  
Jingyun Wang ◽  
Lei Zhang ◽  
Yun Rong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticle ◽  
Quantitative Data ◽  
Visual Inspection ◽  
Sensitivity Enhancement ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157

The size and uniformity of AuNPs were optimized to maximally amplify both visual inspection signals and quantitative data of LFA.

scholarly journals Lateral Flow Immunoassay with the Signal Enhanced by Gold Nanoparticle Aggregates Based on Polyamidoamine Dendrimer

2013 ◽  
Vol 29 (8) ◽  
pp. 799-804 ◽  
Author(s):  
Guangyu SHEN ◽  
Hui XU ◽  
Anant S. GURUNG ◽  
Yunhui YANG ◽  
Guodong LIU
Keyword(s):  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Polyamidoamine Dendrimer ◽  
Nanoparticle Aggregates

scholarly journals Gold nanoparticle-based enhanced lateral flow immunoassay for detection of Cronobacter sakazakii in powdered infant formula

2018 ◽  
Vol 101 (5) ◽  
pp. 3835-3843 ◽  
Author(s):  
Ruili Pan ◽  
Yujun Jiang ◽  
Luhong Sun ◽  
Rui Wang ◽  
Kejin Zhuang ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Infant Formula ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Powdered Infant Formula ◽  
Cronobacter Sakazakii

scholarly journals Comparison of Nanosized Markers in Lateral Flow Immunoassay of Antibiotic Lincomycin

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 41
Author(s):  
Olga D. Hendrickson ◽  
Kseniya V. Serebrennikova ◽  
Elena A. Zvereva ◽  
Demid S. Popravko ◽  
Anatoly V. Zherdev ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Site Testing ◽  
Limits Of Detection ◽  
Chemical Contaminants

Improving the sensitivity of the competitive lateral flow immunoassay (LFIA) is important, given the increasing demands for the monitoring of chemical contaminants in food. The choice of nanosized marker is an essential task for improving the LFIA sensitivity. In this study, a CdSe/ZnS quantum dot (QD)-based LFIA combined with a portable reader was developed for rapid and quantitative detection of an antibiotic lincomycin (LIN). The performance of the proposed fluorescence LFIA was compared to the conventional gold nanoparticle (AuNP)-based LFIA realized with the same immunoreagents. The visual cutoff values were 10 ng/mL for AuNP-based LFIA and 20 ng/mL for QD-based LFIA. Furthermore, the instrumental limits of detection have been shown to be comparable for both nanosized markers and amounted to 0.4 ng/mL for AuNPs and 0.2 ng/mL for QDs, respectively. According to the results obtained, both LFIAs may be used for rapid, cost-effective, on-site testing of antibiotics, in particular LIN. However, the QD-based LFIA exhibits lowest limit of detection with the least immunoreagent consumption, which makes it economically beneficial.

scholarly journals Magnetized Carbon Nanotube Based Lateral Flow Immunoassay for Visual Detection of Complement Factor B

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2759 ◽  
Author(s):  
Yan Huang ◽  
Tingting Wu ◽  
Fang Wang ◽  
Kun Li ◽  
Lisheng Qian ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Human Blood ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Complement Factor ◽  
Factor B ◽  
Complement Factor B ◽  
Flow Test ◽  
Lateral Flow Test

The authors describe a magnetized carbon nanotube (MCNT) based lateral flow immunoassay (LFI) for visual detection of complement factor B (CFB) in blood. MCNT was prepared by decorating magnetic Fe3O4 nanoparticles on multi-walled CNT surface and used as a colored tag for LFI. Monoclonal antibody (mAb, Ab1) of CFB was covalently immobilized on the MCNT surface via diimide-activated conjugation between the carboxyl groups on the MCNT surface and amino groups of antibodies. Polyclonal antibody of CFB (Ab2) and the secondary antibody were used to prepare the lateral flow test strips. The assay involved: (1) the capture of CFB in blood with the mAb-functionalized MCNT; (2) magnetic separation of the formed CFB-mAb-MCNT and excess of mAb-MCNT from the blood with an external magnet; (3) lateral flow test to capture the CFB-mAb-MCNT complex on the test zone and the excess of mAb-MCNT on the control zone; (4) Recording the intensities of the produced the characteristic brown bands with a portable strip reader and quantitating the concentration of CFB. The proof-of-concept was demonstrated by testing CFB in the buffer, and the detection limit was 5 ng mL−1 under the optimized analytical parameters. CFB in 1 μL of human blood was detected successfully in 30 min with this LFI and the results had a high correlation with commercial ELISA kit. Thence, the MCNT-based LFI offers a rapid and low-cost tool for detecting CFB in human blood directly.

Sign in / Sign up

Export Citation Format

Share Document