A glowing antioxidant from tasar silk cocoon

RSC Advances ◽  
2015 ◽  
Vol 5 (126) ◽  
pp. 104563-104573 ◽  
Author(s):  
Tejas Sanjeev Kusurkar ◽  
Anamika Gangwar ◽  
Mangesh Bawankar ◽  
Anupam Mandal ◽  
Dattatraya Dethe ◽  
...  
Keyword(s):  
Cell Line ◽  
Animal Cell ◽  
Fluorescent Dye ◽  
Silk Cocoon ◽  
Animal Cell Line

In this study, a fluorophore can be easily localized inside animal cell line H9c2 using a novel N-TER™ based strategy, and the internalized fluorophore acts both as a fluorescent dye and as an antioxidant.

Serologic and karyologic evidence of incorrect identity of an animal cell line (guinea pig spleen)

In Vitro ◽  
1970 ◽  
Vol 6 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Philip R. Herrick ◽  
Gregory W. Baumann ◽  
Donald J. Merchant ◽  
Marcia C. Shearer ◽  
Chrrles Shipman ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Cell Line ◽  
Animal Cell ◽  
Animal Cell Line

The cell line data base and the new catalogue: Detailed information on 2650 human and animal cell lines

1993 ◽  
Vol 11 (S1) ◽  
pp. S130-S133 ◽  
Author(s):  
A. Manniello ◽  
O. Aresu ◽  
B. Parodi ◽  
P. Romano ◽  
B. Iannotta ◽  
...  
Keyword(s):  
Data Base ◽  
Cell Line ◽  
Cell Lines ◽  
Animal Cell

scholarly journals Influence of culture conditions on Vero cell propagation on non-porous microcarriers

2005 ◽  
Vol 48 (spe) ◽  
pp. 71-77 ◽  
Author(s):  
Marta Cristina de Oliveira Souza ◽  
Marcos da Silva Freire ◽  
Leda dos Reis Castilho
Keyword(s):  
Vero Cell ◽  
Cell Line ◽  
Animal Cell ◽  
Culture Conditions ◽  
World Health ◽  
Adherent Cell ◽  
Scale Production ◽  
Agitation Rate ◽  
High Cell ◽  
Cell Densities

Animal cell cultures are widely employed for the production of viral vaccines and for recombinant protein expression. The cell line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human vaccines. For the large-scale production of vaccines, microcarriers, which are microspheres that serve as support for the cells, are being increasingly used. The use of microcarriers in stirred bioreactors allows high cell densities and, consequently, high virus titres to be achieved. With the aim of selecting appropriate culture conditions for the cultivation of Vero cells at high cell densities, in this work the influence of several variables (agitation rate, ratio of inoculated cells to microcarrier mass and fetal bovine serum concentration) on cell growth on Cytodex 1 microcarriers was studied. Under the best conditions determined, a comparison with Vero cell cultivation on Cytodex 3 microcarriers was carried out.

scholarly journals Recent developments of the cell line integrated molecular authentication database

2016 ◽  
Author(s):  
Paolo D Romano ◽  
Paola Visconti ◽  
Barbara Parodi
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Animal Cell ◽  
Cell Line Authentication ◽  
Molecular Authentication ◽  
Standard Data ◽  
Str Profile ◽  
Frequent Event ◽  
Recent Developments ◽  
Cell Banks

Cross-contamination of human and animal cell lines is a frequent event. For this reason, the results obtained with the same cell lines by different research groups are often not fully comparable, this leading to main reproducibility issues. The short tandem repeat (STR) profile has been proposed as a molecular method for cell line authentication. STR profile standard data sets for human cell lines were proposed by some of the leading cell banks worldwide which also have made the results of STR profiling of their cell lines available on-line. We have built the Cell Line Integrated Molecular Authentication Database (CLIMA) as a reference portal where authentication data are made available to the scientific community. This system, although already largely utilized by researchers from all over the world, presented some limitations and only included a limited amount of STR profiles. Here, we present its most recent developments: the inclusion of additional cell banks and profiles and the availability of a new identification tool.

scholarly journals Advances in paraganglioma–pheochromocytoma cell lines and xenografts

2020 ◽  
Vol 27 (12) ◽  
pp. R433-R450
Author(s):  
Jean-Pierre Bayley ◽  
Peter Devilee
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Animal Cell ◽  
Basic Research ◽  
Cell Models ◽  
Strong Recommendation ◽  
Organoid Culture ◽  
Disease Mechanisms ◽  
Pheochromocytoma Cell ◽  
Cell Line Derivation

This review describes human and rodent-derived cell lines and xenografts developed over the last five decades that are suitable or potentially suitable models for paraganglioma–pheochromocytoma research. We outline the strengths and weaknesses of various models and emphasize the recurring theme that, despite the major challenges involved, more effort is required in the search for valid human and animal cell models of paraganglioma–pheochromocytoma, particularly those relevant to cancers carrying a mutation in one of the succinate dehydrogenase genes. Despite many setbacks, the recent development of a potentially important new model, the RS0 cell line, gives reason for optimism regarding the future of models in the paraganglioma–pheochromocytoma field. We also note that classic approaches to cell line derivation such as SV40-mediated immortalization and newer approaches such as organoid culture or iPSCs have been insufficiently explored. As many existing cell lines have been poorly characterized, we provide recommendations for reporting of paraganglioma and pheochromocytoma cell lines, including the strong recommendation that cell lines are made widely available via the ATCC or a similar cell repository. Basic research in paraganglioma–pheochromocytoma is currently transitioning from the analysis of genetics to the analysis of disease mechanisms and the clinically exploitable vulnerabilities of tumors. A successful transition will require many more disease-relevant human and animal models to ensure continuing progress.

scholarly journals Interlab Cell Line Collection: Bioresource of Established Human and Animal Cell Lines

2015 ◽  
Vol 2 ◽  
Author(s):  
Barbara Parodi ◽  
Ottavia Aresu ◽  
Paola Visconti ◽  
Maria Assunta Manniello ◽  
Paolo Strada
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Animal Cell

scholarly journals Exo70E2 is essential for exocyst subunit recruitment and EXPO formation in both plants and animals

2014 ◽  
Vol 25 (3) ◽  
pp. 412-426 ◽  
Author(s):  
Yu Ding ◽  
Juan Wang ◽  
John Ho Chun Lai ◽  
Vivian Hoi Ling Chan ◽  
Xiangfeng Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Animal Cell ◽  
Resonance Energy Transfer ◽  
Plant Cells ◽  
Resonance Energy ◽  
Single Copy ◽  
Bimolecular Fluorescence Complementation ◽  
Double Membrane ◽  
Animal Cell Line ◽  
Membrane Organelle

In contrast to a single copy of Exo70 in yeast and mammals, the Arabidopsis genome contains 23 paralogues of Exo70 (AtExo70). Using AtExo70E2 and its GFP fusion as probes, we recently identified a novel double-membrane organelle termed exocyst-positive organelle (EXPO) that mediates an unconventional protein secretion in plant cells. Here we further demonstrate that AtExo70E2 is essential for exocyst subunit recruitment and for EXPO formation in both plants and animals. By performing transient expression in Arabidopsis protoplasts, we established that a number of exocyst subunits (especially the members of the Sec family) are unable to be recruited to EXPO in the absence of AtExo70E2. The paralogue AtExo70A1 is unable to substitute for AtExo70E2 in this regard. Fluorescence resonance energy transfer assay and bimolecular fluorescence complementation analyses confirm the interaction between AtExo70E2 and Sec6 and Sec10. AtExo70E2, but not its yeast counterpart, is also capable of inducing EXPO formation in an animal cell line (HEK293A cells). Electron microscopy confirms the presence of double-membraned, EXPO-like structures in HEK293A cells expressing AtExo70E2. Inversely, neither human nor yeast Exo70 homologues cause the formation of EXPO in Arabidopsis protoplasts. These results point to a specific and crucial role for AtExo70E2 in EXPO formation.

Lactate alters plasma membrane potential, increases the concentration of cytosolic Ca2+ and stimulates the secretion of insulin by the hamster β-cell line HIT-T15

1989 ◽  
Vol 3 (2) ◽  
pp. 121-128 ◽  
Author(s):  
J. E. Meats ◽  
M. D. Tuersley ◽  
L. Best ◽  
A. M. Lynch ◽  
S. Tomlinson
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Calcium Channels ◽  
Cell Line ◽  
Intracellular Recording ◽  
Fluorescent Dye ◽  
Β Cell ◽  
Plasma Membrane Potential ◽  
Hit Cells ◽  
Cytosolic Acidification

ABSTRACT We have studied the effect of lactate on a number of intracellular events which may be important in controlling the secretion of insulin by the hamster β-cell line HIT-T15. Using the fluorescent dye Oxonol V, as well as intracellular recording techniques to measure changes in membrane potential, we found that lactate, glucose, K+ and tolbutamide caused depolarization of HIT cells, while valinomycin resulted in hyperpolarization. Consistent with these findings was the observation that 10 mm lactate caused an increase of 69·0±18·4% (s.e.m., n=6) in the level of free cytosolic Ca2+ within HIT cells (assessed by fluorescence of quin 2). This was probably due to influx of Ca2+ through voltage-sensitive calcium channels, since it was dependent upon the concentration of extracellular Ca2+ and inhibited by verapamil. Lactate also caused cytosolic acidification in HIT cells and increased the secretion of insulin. These findings are consistent with the view that the electrogenic efflux of lactate could be a determinant in the activation of HIT cells by lactate and possibly by glucose.

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