Serologic and karyologic evidence of incorrect identity of an animal cell line (guinea pig spleen)

In Vitro ◽  
1970 ◽  
Vol 6 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Philip R. Herrick ◽  
Gregory W. Baumann ◽  
Donald J. Merchant ◽  
Marcia C. Shearer ◽  
Chrrles Shipman ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Cell Line ◽  
Animal Cell ◽  
Animal Cell Line

A glowing antioxidant from tasar silk cocoon

RSC Advances ◽  
2015 ◽  
Vol 5 (126) ◽  
pp. 104563-104573 ◽  
Author(s):  
Tejas Sanjeev Kusurkar ◽  
Anamika Gangwar ◽  
Mangesh Bawankar ◽  
Anupam Mandal ◽  
Dattatraya Dethe ◽  
...  
Keyword(s):  
Cell Line ◽  
Animal Cell ◽  
Fluorescent Dye ◽  
Silk Cocoon ◽  
Animal Cell Line

In this study, a fluorophore can be easily localized inside animal cell line H9c2 using a novel N-TER™ based strategy, and the internalized fluorophore acts both as a fluorescent dye and as an antioxidant.

Growth inhibition by overexpression of human DEAD box protein rck/p54 in cells of a guinea pig cell line

FEBS Letters ◽  
1998 ◽  
Vol 429 (3) ◽  
pp. 279-283 ◽  
Author(s):  
Yukihiro Akao ◽  
Hiromi Mizoguchi ◽  
Nobuko Ohishi ◽  
Kunio Yagi
Keyword(s):  
Guinea Pig ◽  
Growth Inhibition ◽  
Cell Line ◽  
Dead Box ◽  
In Cells

The cell line data base and the new catalogue: Detailed information on 2650 human and animal cell lines

1993 ◽  
Vol 11 (S1) ◽  
pp. S130-S133 ◽  
Author(s):  
A. Manniello ◽  
O. Aresu ◽  
B. Parodi ◽  
P. Romano ◽  
B. Iannotta ◽  
...  
Keyword(s):  
Data Base ◽  
Cell Line ◽  
Cell Lines ◽  
Animal Cell

Guinea pig auditory neurons are protected by glial cell line-derived growth factor from degeneration after noise trauma

1998 ◽  
Vol 124 (1-2) ◽  
pp. 17-26 ◽  
Author(s):  
J Ylikoski ◽  
U Pirvola ◽  
J Virkkala ◽  
P Suvanto ◽  
X.-Q Liang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Guinea Pig ◽  
Cell Line ◽  
Glial Cell ◽  
Auditory Neurons ◽  
Noise Trauma

scholarly journals Infectivity of amastigotes of Trypanosoma cruzi

1986 ◽  
Vol 28 (4) ◽  
pp. 205-212 ◽  
Author(s):  
Tecia Ulisses de Carvalho ◽  
Wanderley de Souza
Keyword(s):  
Trypanosoma Cruzi ◽  
Guinea Pig ◽  
Cell Line ◽  
Peritoneal Macrophages ◽  
Mouse Peritoneal Macrophages ◽  
Pig Serum

The infectivity amastigotes of Trypanosoma cruzi, isolated from the supernatant of the J774G8 macrophage-like cell line infected with trypomastigotes to normal macrophages in vitro was tested. After a period of 1 h of T. cruzi-macrophage interaction about 2% of the mouse peritoneal macrophages had ingested amastigotes. In contrast 12% of the macrophages had ingested epimastigotes. Treatment of the amastigotes with trypsin did not interfere with their ingestion by macrophages. Once inside the macrophages the amastigotes divided and after some days transformed into trypomastigotes. When i.p. inoculated into mice the amastigotes were highly infective, inducing high levels of parasitaemia and tissue parasitism. As previously described for trypomastigotes, amastigotes were not lysed when incubated in the presence of fresh guinea-pig serum. Contrasting with what has been described for trypomastigotes, the resistance of amastigotes to complement-mediated lysis persisted after treatment with trypsin.

scholarly journals Influence of culture conditions on Vero cell propagation on non-porous microcarriers

2005 ◽  
Vol 48 (spe) ◽  
pp. 71-77 ◽  
Author(s):  
Marta Cristina de Oliveira Souza ◽  
Marcos da Silva Freire ◽  
Leda dos Reis Castilho
Keyword(s):  
Vero Cell ◽  
Cell Line ◽  
Animal Cell ◽  
Culture Conditions ◽  
World Health ◽  
Adherent Cell ◽  
Scale Production ◽  
Agitation Rate ◽  
High Cell ◽  
Cell Densities

Animal cell cultures are widely employed for the production of viral vaccines and for recombinant protein expression. The cell line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human vaccines. For the large-scale production of vaccines, microcarriers, which are microspheres that serve as support for the cells, are being increasingly used. The use of microcarriers in stirred bioreactors allows high cell densities and, consequently, high virus titres to be achieved. With the aim of selecting appropriate culture conditions for the cultivation of Vero cells at high cell densities, in this work the influence of several variables (agitation rate, ratio of inoculated cells to microcarrier mass and fetal bovine serum concentration) on cell growth on Cytodex 1 microcarriers was studied. Under the best conditions determined, a comparison with Vero cell cultivation on Cytodex 3 microcarriers was carried out.

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