Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86625-86630 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Xingti Liu ◽  
Zhiyue Gao ◽  
Wei Jiang
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Signal Amplification ◽  
Nicotinamide Adenine ◽  
Label Free ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Exo Iii ◽  
Amplification Strategy

Schematic illustration of the Exo III-assisted amplification strategy for NAD+ detection.

scholarly journals Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 104
Author(s):  
Fuyuan Zhang ◽  
Linyang Liu ◽  
Shengnan Ni ◽  
Jiankang Deng ◽  
Guo-Jun Liu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Aflatoxin M1 ◽  
Exonuclease Iii ◽  
Sensor Performance ◽  
Milk Samples ◽  
Increased Sensitivity ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Optimized Conditions ◽  
Magnetic Nanobeads

In order to satisfy the need for sensitive detection of Aflatoxin M1 (AFM1), we constructed a simple and signal-on fluorescence aptasensor based on an autocatalytic Exonuclease III (Exo III)-assisted signal amplification strategy. In this sensor, the DNA hybridization on magnetic nanobeads could be triggered by the target AFM1, resulting in the release of a single-stranded DNA to induce an Exo III-assisted signal amplification, in which numerous G-quadruplex structures would be produced and then associated with the fluorescent dye to generate significantly amplified fluorescence signals resulting in the increased sensitivity. Under the optimized conditions, this aptasensor was able to detect AFM1 with a practical detection limit of 9.73 ng kg−1 in milk samples. Furthermore, the prepared sensor was successfully used for detection of AFM1 in the commercially available milk samples with the recovery percentages ranging from 80.13% to 108.67%. Also, the sensor performance was evaluated by the commercial immunoassay kit with satisfactory results.

A cascade signal amplification strategy for sensitive and label-free DNA detection based on Exo III-catalyzed recycling coupled with rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2884-2889 ◽  
Author(s):  
Xingti Liu ◽  
Qingwang Xue ◽  
Yongshun Ding ◽  
Jing Zhu ◽  
Lei Wang ◽  
...  
Keyword(s):  
Detection Method ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Free Dna ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Cascade Amplification

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.

Exonuclease III assisted and label-free detection of mercury ion based on toehold strand displacement amplification strategy

2016 ◽  
Vol 8 (39) ◽  
pp. 7054-7060 ◽  
Author(s):  
Lili Yu ◽  
Hui Xu ◽  
Hou Chen ◽  
Liangjiu Bai ◽  
Wenxiang Wang
Keyword(s):  
High Sensitivity ◽  
Mercury Ion ◽  
Fluorometric Assay ◽  
Label Free ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Sensitivity And Selectivity ◽  
Amplification Strategy

A label-free, exonuclease III assisted Hg2+ fluorometric assay based on strand displacement amplification was developed with high sensitivity and selectivity.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

Ultrasensitive and simple fluorescence biosensor for detection of the mecA gene of Staphylococcus aureus by using an exonuclease III-assisted cascade signal amplification strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5670-5675 ◽  
Author(s):  
Qiong Li ◽  
Danhua Zhou ◽  
Jiafeng Pan ◽  
Zhi Liu ◽  
Junhua Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Signal Amplification ◽  
Meca Gene ◽  
Exonuclease Iii ◽  
G Quadruplex ◽  
Exo Iii ◽  
Fluorescence Biosensor ◽  
Amplification Strategy

An ultrasensitive biosensor was developed for the detection of the mecA gene of Staphylococcus aureus based on the Exo III and G-quadruplex.

Label-free and highly sensitive fluorescence detection of lead(ii) based on DNAzyme and exonuclease III-assisted cascade signal amplification

2019 ◽  
Vol 43 (15) ◽  
pp. 5857-5862 ◽  
Author(s):  
Jiafeng Pan ◽  
Qiong Li ◽  
Danhua Zhou ◽  
Junhua Chen
Keyword(s):  
Fluorescence Detection ◽  
Signal Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Exo Iii

A Pb2+ biosensor has been constructed based on Exo III-assisted cascade signal amplification using 2-amino-5,6,7-trimethyl-1,8-naphthyridine as the signal indicator.

scholarly journals Sensitive and Label-free Detection of Bacteria in Osteomyelitis through Exo III-Assisted Cascade Signal Amplification

ACS Omega ◽  
2021 ◽  
Author(s):  
Yuanyuan Li ◽  
Fei Xu ◽  
Jinhua Zhang ◽  
Jinshan Huang ◽  
Di Shen ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Label Free ◽  
Label Free Detection ◽  
Exo Iii
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