Exonuclease III assisted and label-free detection of mercury ion based on toehold strand displacement amplification strategy

2016 ◽  
Vol 8 (39) ◽  
pp. 7054-7060 ◽  
Author(s):  
Lili Yu ◽  
Hui Xu ◽  
Hou Chen ◽  
Liangjiu Bai ◽  
Wenxiang Wang
Keyword(s):  
High Sensitivity ◽  
Mercury Ion ◽  
Fluorometric Assay ◽  
Label Free ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Sensitivity And Selectivity ◽  
Amplification Strategy

A label-free, exonuclease III assisted Hg2+ fluorometric assay based on strand displacement amplification was developed with high sensitivity and selectivity.

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86625-86630 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Xingti Liu ◽  
Zhiyue Gao ◽  
Wei Jiang
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Signal Amplification ◽  
Nicotinamide Adenine ◽  
Label Free ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Exo Iii ◽  
Amplification Strategy

Schematic illustration of the Exo III-assisted amplification strategy for NAD+ detection.

Visual/CVG-AFS/ICP-MS multi-mode and label-free detection of target nucleic acids based on a selective cation exchange reaction and enzyme-free strand displacement amplification

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4407-4412 ◽  
Author(s):  
Rui Dai ◽  
Pingyue Hu ◽  
Xiu Wang ◽  
Shixin Wang ◽  
Xinmei Song ◽  
...  
Keyword(s):  
Cation Exchange ◽  
Exchange Reaction ◽  
Label Free ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Mode Detection ◽  
Icp Ms ◽  
Label Free Detection ◽  
Cation Exchange Reaction ◽  
Multi Mode

Visual/CVG-AFS/ICP-MS three-mode detection of DNA based on the selective cation exchange reaction and enzyme-free strand displacement amplification.

scholarly journals Chemical-free and scalable process for the fabrication of a uniform array of liquid-gated CNTFET, evaluated by KCl electrolyte

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pankaj B. Agarwal ◽  
Navneet Kumar Thakur ◽  
Rishi Sharma ◽  
Parul Singh ◽  
Joshy Joseph ◽  
...  
Keyword(s):  
Field Effect Transistors ◽  
Reference Electrode ◽  
High Sensitivity ◽  
Label Free ◽  
Practical Applications ◽  
Lithography Process ◽  
Free Process ◽  
Label Free Detection ◽  
Sensitivity And Selectivity ◽  
Uniform Array

AbstractBiosensors based on liquid-gated carbon nanotubes field-effect transistors (LG-CNTFETs) have attracted considerable attention, as they offer high sensitivity and selectivity; quick response and label-free detection. However, their practical applications are limited due to the numerous fabrication challenges including resist-based lithography, in which after the lithography process, the resist leaves trace level contaminations over the CNTs that affect the performance of the fabricated biosensors. Here, we report the realization of LG-CNTFET devices using silicon shadow mask-based chemical-free lithography process on a 3-in. silicon wafer, yielding 21 sensor chips. Each sensor chip consists of 3 × 3 array of LG-CNTFET devices. Field emission scanning electron microscope (FESEM) and Raman mapping confirm the isolation of devices within the array chip having 9 individual devices. A reference electrode (Ag/AgCl) is used to demonstrate the uniformity of sensing performances among the fabricated LG-CNTFET devices in an array using different KCl molar solutions. The average threshold voltage (Vth) for all 9 devices varies from 0.46 to 0.19 V for 0.1 mM to 1 M KCl concentration range. This developed chemical-free process of LG-CNTFET array fabrication is simple, inexpensive, rapid having a commercial scope and thus opens a new realm of scalable realization of various biosensors.

scholarly journals Optical Sensing of Microbial Life on Surfaces

2015 ◽  
Vol 82 (5) ◽  
pp. 1362-1371 ◽  
Author(s):  
M. Fischer ◽  
G. J. Triggs ◽  
T. F. Krauss
Keyword(s):  
Basic Research ◽  
High Sensitivity ◽  
Natural Environments ◽  
Label Free ◽  
Biofilm Growth ◽  
Microbial Cells ◽  
Industrial Facilities ◽  
Label Free Detection ◽  
Sensitivity And Selectivity ◽  
The Many

ABSTRACTThe label-free detection of microbial cells attached to a surface is an active field of research. The field is driven by the need to understand and control the growth of biofilms in a number of applications, including basic research in natural environments, industrial facilities, and clinical devices, to name a few. Despite significant progress in the ability to monitor the growth of biofilms and related living cells, the sensitivity and selectivity of such sensors are still a challenge. We believe that among the many different technologies available for monitoring biofilm growth, optical techniques are the most promising, as they afford direct imaging and offer high sensitivity and specificity. Furthermore, as each technique offers different insights into the biofilm growth mechanism, our analysis allows us to provide an overview of the biological processes at play. In addition, we use a set of key parameters to compare state-of-the-art techniques in the field, including a critical assessment of each method, to identify the most promising types of sensors. We highlight the challenges that need to be overcome to improve the characteristics of current biofilm sensor technologies and indicate where further developments are required. In addition, we provide guidelines for selecting a suitable sensor for detecting microbial cells on a surface.

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