Label-free and highly sensitive fluorescence detection of lead(ii) based on DNAzyme and exonuclease III-assisted cascade signal amplification

2019 ◽  
Vol 43 (15) ◽  
pp. 5857-5862 ◽  
Author(s):  
Jiafeng Pan ◽  
Qiong Li ◽  
Danhua Zhou ◽  
Junhua Chen
Keyword(s):  
Fluorescence Detection ◽  
Signal Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Exo Iii

A Pb2+ biosensor has been constructed based on Exo III-assisted cascade signal amplification using 2-amino-5,6,7-trimethyl-1,8-naphthyridine as the signal indicator.

Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86625-86630 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Xingti Liu ◽  
Zhiyue Gao ◽  
Wei Jiang
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Signal Amplification ◽  
Nicotinamide Adenine ◽  
Label Free ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Exo Iii ◽  
Amplification Strategy

Schematic illustration of the Exo III-assisted amplification strategy for NAD+ detection.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor

Nanoscale ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 3055-3058 ◽  
Author(s):  
Xinjian Yang ◽  
Zhiqiang Gao
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Signal Amplification ◽  
Colorimetric Assay ◽  
Folate Receptor ◽  
Colorimetric Detection ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor.

Polydopamine nanotube mediated fluorescent biosensor for Hg(ii) determination through exonuclease III-assisted signal amplification

The Analyst ◽  
2018 ◽  
Vol 143 (11) ◽  
pp. 2623-2631 ◽  
Author(s):  
Ravikumar A ◽  
Panneerselvam P
Keyword(s):  
Aqueous Solution ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Biosensor ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Fluorescence Biosensor

We describe a highly sensitive fluorescence biosensor incorporating polydopamine nanotubes (PDNTs) based on the mechanism of exonuclease III (Exo III) assisted signal amplification for the determination of Hg2+ in aqueous solution.

Target-induced structure switching of DNA for label-free and ultrasensitive electrochemiluminescent detection of proteins

2014 ◽  
Vol 50 (24) ◽  
pp. 3211-3213 ◽  
Author(s):  
Mengli Yang ◽  
Ying Chen ◽  
Yun Xiang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Dna Structure ◽  
Label Free ◽  
Switching Strategy ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Exo Iii ◽  
Recycling Amplification ◽  
Structure Switching ◽  
Induced Structure

Highly sensitive and label-free detection of thrombin is achieved via a target-induced DNA structure switching strategy and Exo III-assisted recycling amplification.

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

Target-activated cascaded digestion amplification of exonuclease III aided signal-on and ultrasensitive fluorescence detection of ATP

2018 ◽  
Vol 42 (5) ◽  
pp. 3534-3540 ◽  
Author(s):  
Xueqi Leng ◽  
Rongguo Li ◽  
Yu Wang ◽  
Yunping Wu ◽  
Yuqin Tu ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Fluorescence Detection ◽  
Fluorescence Sensing ◽  
Exonuclease Iii ◽  
One Step ◽  
Exo Iii

In this work, a rapid, one-step and ultrasensitive signal-on fluorescence sensing for the detection of adenosine triphosphate (ATP) based on target-activated cascaded digestion amplification with Exo III aid was developed.

scholarly journals Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 104
Author(s):  
Fuyuan Zhang ◽  
Linyang Liu ◽  
Shengnan Ni ◽  
Jiankang Deng ◽  
Guo-Jun Liu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Aflatoxin M1 ◽  
Exonuclease Iii ◽  
Sensor Performance ◽  
Milk Samples ◽  
Increased Sensitivity ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Optimized Conditions ◽  
Magnetic Nanobeads

In order to satisfy the need for sensitive detection of Aflatoxin M1 (AFM1), we constructed a simple and signal-on fluorescence aptasensor based on an autocatalytic Exonuclease III (Exo III)-assisted signal amplification strategy. In this sensor, the DNA hybridization on magnetic nanobeads could be triggered by the target AFM1, resulting in the release of a single-stranded DNA to induce an Exo III-assisted signal amplification, in which numerous G-quadruplex structures would be produced and then associated with the fluorescent dye to generate significantly amplified fluorescence signals resulting in the increased sensitivity. Under the optimized conditions, this aptasensor was able to detect AFM1 with a practical detection limit of 9.73 ng kg−1 in milk samples. Furthermore, the prepared sensor was successfully used for detection of AFM1 in the commercially available milk samples with the recovery percentages ranging from 80.13% to 108.67%. Also, the sensor performance was evaluated by the commercial immunoassay kit with satisfactory results.

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