Monoclonal antibody–europium conjugate-based lateral flow time-resolved fluoroimmunoassay for quantitative determination of T-2 toxin in cereals and feed

2015 ◽  
Vol 7 (6) ◽  
pp. 2822-2829 ◽  
Author(s):  
Zhaowei Zhang ◽  
Du Wang ◽  
Jing Li ◽  
Qi Zhang ◽  
Peiwu Li
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Flow Time ◽  
Lateral Flow ◽  
Ultrasensitive Detection ◽  
Time Resolved

A method of lateral flow time-resolved fluoroimmunoassay (LF-TRFIA) was developed for rapid and ultrasensitive detection of T-2 toxin with TRFIA strips and an assorted portable TRFIA reader.

A simple time-resolved fluorescence assay for quantitative determination of DOTA chelator

2019 ◽  
Vol 584 ◽  
pp. 113384 ◽  
Author(s):  
Michał Szkop ◽  
Kamil Brygoła ◽  
Magdalena Janczewska ◽  
Tomasz Ciach
Keyword(s):  
Quantitative Determination ◽  
Fluorescence Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved

scholarly journals Direct Assay for Cobalamin Bound to Transcobalamin (Holo-Transcobalamin) in Serum

2002 ◽  
Vol 48 (3) ◽  
pp. 526-532 ◽  
Author(s):  
Marius Ulleland ◽  
Ingar Eilertsen ◽  
Edward V Quadros ◽  
Sheldon P Rothenberg ◽  
Sergey N Fedosov ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Detection Limit ◽  
Quantitative Determination ◽  
Reliable Method ◽  
Solid Phase ◽  
Reference Interval ◽  
Magnetic Microspheres ◽  
Affinity Constant ◽  
Capture Assay

Abstract Background: Only cobalamin carried by transcobalamin (holo-transcobalamin) is available for cellular uptake and hence is physiologically relevant. However, no reliable or accurate methods for quantifying holo-transcobalamin are available. We report a novel holo-transcobalamin assay based on solid-phase capture of transcobalamin. Methods: A monoclonal antibody specific for human transcobalamin with an affinity constant >1010 L/mol was immobilized on magnetic microspheres to capture and concentrate transcobalamin. The cobalamin bound to transcobalamin was then released and assayed by a competitive binding radioassay. The quantification of holo-transcobalamin was accomplished using calibrators composed of recombinant, human holo-transcobalamin. Results: The assay was specific for holo-transcobalamin and had a detection limit of 5 pmol/L. Within-run and total imprecision (CV) was 5% and 8–9%, respectively. The working range (CV <20%) was 5–370 pmol/L. Dilutions of serum were linear in the assay range. The recovery of recombinant, human holo-transcobalamin added to serum was 93–108%. A 95% reference interval of 24–157 pmol/L was established for holo-transcobalamin in 105 healthy volunteers 20–80 years of age. For 72 of these sera, holo-haptocorrin and total cobalamin were also determined. Whereas holo-haptocorrin correlated well (r2 = 0.87) with total cobalamin, holo-transcobalamin correlated poorly (r2 = 0.23) with total cobalamin or holo-haptocorrin. Conclusions: The solid-phase capture assay provides a simple, reliable method for quantitative determination of holo-transcobalamin in serum.

scholarly journals Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues

2013 ◽  
Vol 58 (15) ◽  
pp. 1838-1842 ◽  
Author(s):  
Shu Wei ◽  
Tao Le ◽  
Yong Chen ◽  
Jian Xu ◽  
HongQiu He ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Animal Tissues ◽  
Time Resolved

Quantitative Determination of Thiabendazole in Fruit Juices and Bulk Juice Concentrates Using a Thiabendazole Monoclonal Antibody

1995 ◽  
Vol 43 (5) ◽  
pp. 1407-1412 ◽  
Author(s):  
Rodney J. Bushway ◽  
David L. Brandon ◽  
Anne H. Bates ◽  
Lei Li ◽  
Karen A. Larkin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Fruit Juices

A novel bifunctional europium chelate applied in quantitative determination of human immunoglobin G using time-resolved fluoroimmunoassay

2011 ◽  
Vol 409 (2) ◽  
pp. 244-248 ◽  
Author(s):  
Cheng-Gang Niu ◽  
Jing Liu ◽  
Pin-Zhu Qin ◽  
Guang-Ming Zeng ◽  
Min Ruan ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Time Resolved ◽  
Europium Chelate ◽  
Immunoglobin G
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