Quantitative Determination of Thiabendazole in Fruit Juices and Bulk Juice Concentrates Using a Thiabendazole Monoclonal Antibody

1995 ◽  
Vol 43 (5) ◽  
pp. 1407-1412 ◽  
Author(s):  
Rodney J. Bushway ◽  
David L. Brandon ◽  
Anne H. Bates ◽  
Lei Li ◽  
Karen A. Larkin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Fruit Juices

scholarly journals Direct Assay for Cobalamin Bound to Transcobalamin (Holo-Transcobalamin) in Serum

2002 ◽  
Vol 48 (3) ◽  
pp. 526-532 ◽  
Author(s):  
Marius Ulleland ◽  
Ingar Eilertsen ◽  
Edward V Quadros ◽  
Sheldon P Rothenberg ◽  
Sergey N Fedosov ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Detection Limit ◽  
Quantitative Determination ◽  
Reliable Method ◽  
Solid Phase ◽  
Reference Interval ◽  
Magnetic Microspheres ◽  
Affinity Constant ◽  
Capture Assay

Abstract Background: Only cobalamin carried by transcobalamin (holo-transcobalamin) is available for cellular uptake and hence is physiologically relevant. However, no reliable or accurate methods for quantifying holo-transcobalamin are available. We report a novel holo-transcobalamin assay based on solid-phase capture of transcobalamin. Methods: A monoclonal antibody specific for human transcobalamin with an affinity constant >1010 L/mol was immobilized on magnetic microspheres to capture and concentrate transcobalamin. The cobalamin bound to transcobalamin was then released and assayed by a competitive binding radioassay. The quantification of holo-transcobalamin was accomplished using calibrators composed of recombinant, human holo-transcobalamin. Results: The assay was specific for holo-transcobalamin and had a detection limit of 5 pmol/L. Within-run and total imprecision (CV) was 5% and 8–9%, respectively. The working range (CV <20%) was 5–370 pmol/L. Dilutions of serum were linear in the assay range. The recovery of recombinant, human holo-transcobalamin added to serum was 93–108%. A 95% reference interval of 24–157 pmol/L was established for holo-transcobalamin in 105 healthy volunteers 20–80 years of age. For 72 of these sera, holo-haptocorrin and total cobalamin were also determined. Whereas holo-haptocorrin correlated well (r2 = 0.87) with total cobalamin, holo-transcobalamin correlated poorly (r2 = 0.23) with total cobalamin or holo-haptocorrin. Conclusions: The solid-phase capture assay provides a simple, reliable method for quantitative determination of holo-transcobalamin in serum.

Monoclonal antibody–europium conjugate-based lateral flow time-resolved fluoroimmunoassay for quantitative determination of T-2 toxin in cereals and feed

2015 ◽  
Vol 7 (6) ◽  
pp. 2822-2829 ◽  
Author(s):  
Zhaowei Zhang ◽  
Du Wang ◽  
Jing Li ◽  
Qi Zhang ◽  
Peiwu Li
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Flow Time ◽  
Lateral Flow ◽  
Ultrasensitive Detection ◽  
Time Resolved

A method of lateral flow time-resolved fluoroimmunoassay (LF-TRFIA) was developed for rapid and ultrasensitive detection of T-2 toxin with TRFIA strips and an assorted portable TRFIA reader.

Quantitative determination of glucose in blood plasma and in fruit juices by combined WATR-CPMG 1H NMR spectroscopy

1992 ◽  
Vol 64 (21) ◽  
pp. 2570-2574 ◽  
Author(s):  
Shiyan. Fan ◽  
W. Y. Choy ◽  
S. L. Lam ◽  
S. C. F. Au-Yeung ◽  
L. Tsang ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Quantitative Determination ◽  
Blood Plasma ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Fruit Juices ◽  
In Blood Plasma

Quantitative determination of phenolic antioxidants in fruit juices by GC-MS/MS using automated injector port silylation after QuEChERS extraction

2021 ◽  
Vol 160 ◽  
pp. 105705
Author(s):  
Manoj Kumar Gupta ◽  
Astitva Anand ◽  
Ankita Asati ◽  
Ramya Thati ◽  
Kumar Katragunta ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Fruit Juices ◽  
Phenolic Antioxidants

The use of stable isotopes ratios for authentication of fruit juices

2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Dana Magdas ◽  
Nicoleta Vedeanu ◽  
Romulus Puscas
Keyword(s):  
Stable Isotopes ◽  
Quantitative Determination ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Tap Water ◽  
Co2 Assimilation ◽  
Fruit Juices ◽  
Added Sugar ◽  
Δ 13C

AbstractThe determination of the content of stable isotopes, 18O and 2H, respectively, in juice water facilitates the distinction between authentic juices and juices made from concentrates by redilution with tap water. At the same time, the detection of C4 cane or corn-derived sugar syrups in fruit juices which are produced from C3 fruit types is thus facilitated by the characteristic differences in 13C/12C, expressed as δ 13C (‰) values due to photosynthetic CO2 assimilation via the C3−, C4−, and crassulacean acid metabolism pathways. In this study, the quantitative determination of water added to an authentic juice, on the basis of δ 18O, and δ 2H values, respectively, was successfully performed. Also, the δ 18O, and δ 2H of juice water and δ 13C of the whole juice in 18 samples were also determined. The results obtained provided us with the possibility of distinguishing between authentic fruit juices and those obtained by redilution of concentrated fruit juices and the detection of C4 type added sugar.

Determination of Ochratoxin A by Monoclonal Antibody-Based Enzyme Immunoassay

1988 ◽  
Vol 71 (5) ◽  
pp. 961-964 ◽  
Author(s):  
Alan A G Candlish ◽  
William H Stimson ◽  
John E Smith
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Ochratoxin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody ◽  
Buffer Solution ◽  
Bicarbonate Buffer ◽  
High Affinity ◽  
Successful Production

Abstract A simple and rapid indirect enzyme-linked immunosorbent assay was developed for the quantitative determination of ochratoxin A in barley after the successful production of a high affinity, specific monoclonal antibody. A rapid sample cleanup was achieved by extracting ochratoxin A from barley with chloroform and partitioning the toxin into bicarbonate buffer; the buffer solution was then added directly to the assay plate and ochratoxin A content was assessed. Recoveries were greater than 85% and detection limits were 5 μg ochratoxin A/ kg barley.

Rapid quantitative determination of fentanyl in human urine and serum using a gold-based immunochromatographic strip sensor

2020 ◽  
Vol 8 (37) ◽  
pp. 8573-8584
Author(s):  
Xianlu Lei ◽  
Xinxin Xu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Liguang Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Immunosorbent Assay ◽  
Human Urine ◽  
Colloidal Gold ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Human Urine And Serum ◽  
Urine And Serum

In this study, an ultrasensitive monoclonal antibody (mAb) was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold-based immunochromatographic strip (CG-ICS) for the analysis of fentanyl in urine and serum.

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