Electrophoretic Method for the Quantitative Determination of a Benzyl-DTPA Ligand in DTPA Monoclonal Antibody Conjugates

1995 ◽  
Vol 6 (3) ◽  
pp. 313-315 ◽  
Author(s):  
Diep T. Pham ◽  
Frans M. Kaspersen ◽  
Ebo S. Bos
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Electrophoretic Method ◽  
Antibody Conjugates

A Rapid Electrophoretic Method for the Determination of the Isoenzymes of Serum Lactate Dehydrogenase

1966 ◽  
Vol 12 (5) ◽  
pp. 308-313 ◽  
Author(s):  
Albert W Opher ◽  
Charles S Collier ◽  
Joseph M Miller
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Quantitative Determination ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Electrophoretic Method ◽  
The Individual ◽  
Electrophoretic Procedure ◽  
Purple Formazan

Abstract A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore III strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.

Quantitative Nephelometric Microdetermination of Alpha-Globulins in Serum and Cerebrospinal Fluid

1956 ◽  
Vol 2 (3) ◽  
pp. 195-213 ◽  
Author(s):  
Abraham Saifer ◽  
Michael C Zymaris
Keyword(s):  
Cerebrospinal Fluid ◽  
Quantitative Determination ◽  
Salt Concentration ◽  
Protein Fraction ◽  
Specific Interaction ◽  
Normal Serum ◽  
Electrophoretic Method ◽  
Serum Fractions ◽  
Standard Serum

Abstract 1. A micronephelometric method is presented for the quantitative determination of total α-globulins in both serum and cerebrospinal fluid. The method is based on the relatively specific interaction of this protein fraction with the cationic detergent, Octab, at pH 6.65 in collidine-NaCl (.08M) buffer. 2. The various Cohn's protein fractions (Method 6) (25) were investigated with the nephelometric procedure. In comparison with normal serum, Fractions IV-6, IV-1, and IV-4, respectively showed the greatest detergent reactivity per milligram of α-globulin. 3. The variations of such factors as the concentrations of reagents, protein/detergent ratio, and salt concentration were reinvestigated. 4. Analysis of 14 normal and 33 pathologic sera gave excellent checks with the total α-globulin values obtained with the Tiselius electrophoretic method. 5. Application of the procedure to the analysis of the α-globulin content of cerebrospinal fluid required the addition of standard serum to 0.5-ml. aliquots of spinal fluid for stoichiometric results.

scholarly journals Direct Assay for Cobalamin Bound to Transcobalamin (Holo-Transcobalamin) in Serum

2002 ◽  
Vol 48 (3) ◽  
pp. 526-532 ◽  
Author(s):  
Marius Ulleland ◽  
Ingar Eilertsen ◽  
Edward V Quadros ◽  
Sheldon P Rothenberg ◽  
Sergey N Fedosov ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Detection Limit ◽  
Quantitative Determination ◽  
Reliable Method ◽  
Solid Phase ◽  
Reference Interval ◽  
Magnetic Microspheres ◽  
Affinity Constant ◽  
Capture Assay

Abstract Background: Only cobalamin carried by transcobalamin (holo-transcobalamin) is available for cellular uptake and hence is physiologically relevant. However, no reliable or accurate methods for quantifying holo-transcobalamin are available. We report a novel holo-transcobalamin assay based on solid-phase capture of transcobalamin. Methods: A monoclonal antibody specific for human transcobalamin with an affinity constant >1010 L/mol was immobilized on magnetic microspheres to capture and concentrate transcobalamin. The cobalamin bound to transcobalamin was then released and assayed by a competitive binding radioassay. The quantification of holo-transcobalamin was accomplished using calibrators composed of recombinant, human holo-transcobalamin. Results: The assay was specific for holo-transcobalamin and had a detection limit of 5 pmol/L. Within-run and total imprecision (CV) was 5% and 8–9%, respectively. The working range (CV <20%) was 5–370 pmol/L. Dilutions of serum were linear in the assay range. The recovery of recombinant, human holo-transcobalamin added to serum was 93–108%. A 95% reference interval of 24–157 pmol/L was established for holo-transcobalamin in 105 healthy volunteers 20–80 years of age. For 72 of these sera, holo-haptocorrin and total cobalamin were also determined. Whereas holo-haptocorrin correlated well (r2 = 0.87) with total cobalamin, holo-transcobalamin correlated poorly (r2 = 0.23) with total cobalamin or holo-haptocorrin. Conclusions: The solid-phase capture assay provides a simple, reliable method for quantitative determination of holo-transcobalamin in serum.

Monoclonal antibody–europium conjugate-based lateral flow time-resolved fluoroimmunoassay for quantitative determination of T-2 toxin in cereals and feed

2015 ◽  
Vol 7 (6) ◽  
pp. 2822-2829 ◽  
Author(s):  
Zhaowei Zhang ◽  
Du Wang ◽  
Jing Li ◽  
Qi Zhang ◽  
Peiwu Li
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Flow Time ◽  
Lateral Flow ◽  
Ultrasensitive Detection ◽  
Time Resolved

A method of lateral flow time-resolved fluoroimmunoassay (LF-TRFIA) was developed for rapid and ultrasensitive detection of T-2 toxin with TRFIA strips and an assorted portable TRFIA reader.

Quantitative Determination of Thiabendazole in Fruit Juices and Bulk Juice Concentrates Using a Thiabendazole Monoclonal Antibody

1995 ◽  
Vol 43 (5) ◽  
pp. 1407-1412 ◽  
Author(s):  
Rodney J. Bushway ◽  
David L. Brandon ◽  
Anne H. Bates ◽  
Lei Li ◽  
Karen A. Larkin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Fruit Juices

scholarly journals Responsive Antibody Conjugates Enable Quantitative Determination of Intracellular Bond Degradation Rate

2019 ◽  
Vol 26 (12) ◽  
pp. 1643-1651.e4 ◽  
Author(s):  
Michelle R. Sorkin ◽  
Joshua A. Walker ◽  
Sneha R. Kabaria ◽  
Nicole P. Torosian ◽  
Christopher A. Alabi
Keyword(s):  
Quantitative Determination ◽  
Degradation Rate ◽  
Bond Degradation ◽  
Antibody Conjugates
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