A Novel Method for Quantitative Analysis of Anti-MUC1 Expressing Ovarian Cancer Cell Surface Based on Magnetic Cell Separation

2012 ◽  
Vol 12 (8) ◽  
pp. 256-266 ◽  
Author(s):  
Daryoush Shahbazi-G ◽  
Mohammad Abdolahi
Keyword(s):  
Ovarian Cancer ◽  
Quantitative Analysis ◽  
Cell Surface ◽  
Cancer Cell ◽  
Cell Separation ◽  
Ovarian Cancer Cell ◽  
Magnetic Cell Separation ◽  
Novel Method

Upregulation of FasL by LPA on ovarian cancer cell surface leads to apoptosis of activated lymphocytes

2004 ◽  
Vol 95 (3) ◽  
pp. 488-495 ◽  
Author(s):  
Yuru Meng ◽  
Laura Graves ◽  
Thuy-Vy Do ◽  
John So ◽  
David A. Fishman
Keyword(s):  
Ovarian Cancer ◽  
Cell Surface ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Activated Lymphocytes

Human monoclonal antibody developed against ovarian cancer cell surface antigen

Cancer ◽  
1994 ◽  
Vol 73 (S3) ◽  
pp. 1098-1104
Author(s):  
Tandra R. Chaudhuri ◽  
Kurt R. Zinn ◽  
J. Steven Morris ◽  
Gregory A. McDonald ◽  
Alfred S. Llorens ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Monoclonal Antibody ◽  
Cell Surface ◽  
Cancer Cell ◽  
Surface Antigen ◽  
Ovarian Cancer Cell ◽  
Human Monoclonal Antibody ◽  
Cell Surface Antigen

scholarly journals Patient-derived and artificial ascites have minor effects on MeT-5A mesothelial cells and do not facilitate ovarian cancer cell adhesion

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0241500
Author(s):  
Manuela Estermann ◽  
Yen-Lin Huang ◽  
Dedy Septiadi ◽  
Danilo Ritz ◽  
Ching-Yeu Liang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Adhesion ◽  
Cell Surface ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Cell Layer ◽  
Mesothelial Cell ◽  
Cell Interactions ◽  
Artificial Ascites ◽  
Cancer Cell Adhesion

The presence of ascites in the peritoneal cavity leads to morphological and functional changes of the peritoneal mesothelial cell layer. Cells loose cell-cell interactions, rearrange their cytoskeleton, activate the production of fibronectin, and change their cell surface morphology in a proinflammatory environment. Moreover, ovarian cancer cell adhesion has been shown to be facilitated by these changes due to increased integrin- and CD44-mediated binding sites. In this study, the biological responsiveness of the human pleural mesothelial cell line MeT-5A to patient-derived and artificial ascites was studied in vitro and adhesion of ovarian cancer cells, i.e. SKOV-3 cells, investigated. Changes were mainly observed in cells exposed to artificial ascites containing higher cytokine concentrations than patient-derived ascites. Interestingly, reduced cell-cell interactions were already observed in untreated MeT-5A cells and effects on tight junction protein expression and permeability upon exposure to ascites were minor. Ascites induced upregulation of CDC42 effector protein 2 expression, which affects stress fiber formation, however significant F-actin reorganization was not observed. Moreover, fibronectin production remained unchanged. Analysis of mesothelial cell surface characteristics showed upregulated expression of intercellular adhesion molecule 1, slightly increased hyaluronic acid secretion and decreased microvillus expression upon exposure to ascites. Nevertheless, the observed changes were not sufficient to facilitate adhesion of SKOV-3 cells on MeT-5A cell layer. This study revealed that MeT-5A cells show a reduced biological responsiveness to the presence of ascites, in contrast to published studies on primary human peritoneal mesothelial cells.

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