scholarly journals In vivo imaging of cancer cell size and cellularity using temporal diffusion spectroscopy

2016 ◽  
Vol 78 (1) ◽  
pp. 156-164 ◽  
Author(s):  
Xiaoyu Jiang ◽  
Hua Li ◽  
Jingping Xie ◽  
Eliot T. McKinley ◽  
Ping Zhao ◽  
...  
Keyword(s):  
Cell Size ◽  
Cancer Cell ◽  
In Vivo Imaging

In vivo imaging of nuclear-cytoplasmic deformation and partition during cancer cell death due to immune rejection

2012 ◽  
Vol 113 (2) ◽  
pp. 465-472 ◽  
Author(s):  
Yasuyuki Amoh ◽  
Yuko Hamada ◽  
Kensei Katsuoka ◽  
Robert M. Hoffman
Keyword(s):  
Cell Death ◽  
Cancer Cell ◽  
In Vivo Imaging ◽  
Immune Rejection ◽  
Cancer Cell Death

Subcellular real-time in vivo imaging of intralymphatic and intravascular cancer-cell trafficking

2008 ◽  
Author(s):  
M. McElroy ◽  
K. Hayashi ◽  
S. Kaushal ◽  
M. Bouvet ◽  
Robert M. Hoffman
Keyword(s):  
Real Time ◽  
Cancer Cell ◽  
In Vivo Imaging ◽  
Cell Trafficking

Dynamic In Vivo Imaging Reveals That Leukemia-Induced Changes in the Bone Marrow Microenvironment Alter Mechanisms of Metastasis.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2805-2805
Author(s):  
Angela Colmone ◽  
Veena Krishnamoorthy ◽  
Dorothy A. Sipkins
Keyword(s):  
Bone Marrow ◽  
Tumor Growth ◽  
Cancer Cell ◽  
In Vivo Imaging ◽  
Lymphoblastic Leukemia ◽  
Tumor Margin ◽  
Metastatic Process ◽  
Induced Changes ◽  
The Impact

Abstract Tissue microenvironments have been shown to critically regulate cancer cell survival and proliferation. Conversely, while tumor growth can induce neovascularization, the impact of other tumor-induced changes in the microenvironment are less well understood. Here we utilize a xenograft model of Nalm-6 pre-B acute lymphoblastic leukemia (ALL) in SCID mice to examine how tumor growth alters the bone marrow (BM) microenvironment. Using dynamic confocal and multiphoton in vivo imaging, we find that tumor growth dramatically down-regulates expression of the chemokine SDF-1 in the BM microvasculature in areas of leukemic proliferation. SDF-1 has been shown to play a key role in cancer cell metastasis for numerous cell types including Nalm-6, and directs initial Nalm-6 homing to specific SDF-1-positive vascular beds. In contrast, we found that Nalm-6 introduced in mice previously engrafted with leukemia home to SDF-1-negative vasculature, predominantly at the advancing tumor margin. Furthermore, inhibition of chemokine signaling by pertussis toxin pre-treatment of Nalm-6 cells demonstrates that Nalm-6 homing in engrafted mice is chemokine-independent. In summary, these findings suggest that leukemic growth profoundly alters the mechanisms underlying the metastatic process by inducing changes in the host vascular microenvironment. These changes may increase the efficiency of the metastatic process and/or the advancement of the tumor margin. Therapies directed at blocking tumor metastases, therefore, may need to be tailored according to tumor stage.

Effect of anti-β1 integrin antibody on lung seeding of osteosarcoma cells in live mice visualized by single-cell in vivo imaging.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 10072-10072
Author(s):  
Hiroaki Kimura ◽  
Yasunori Tome ◽  
Masashi Momiyama ◽  
Katsuhiro Hayashi ◽  
Michael Bouvet ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Cancer Cells ◽  
Cancer Cell ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Β1 Integrin ◽  
Cell Seeding ◽  
Osteosarcoma Cells ◽  
Single Cancer

10072 Background: Integrins play a role in tumor growth and metastasis (Int. J. Cancer 129, 2905-2915, 2011). However, the effect of integrin inhibition has not been visualized on single cancer cells in vivo. In this study, we used a powerful subcellular in vivo imaging model to demonstrate how an anti-integrin antibody affects seeding and growth of osteosarcoma cells on the lung. Methods: The 143B human osteosarcoma cell line expressing red fluorescent protein (RFP) in the cytoplasm and green fluorescent protein (GFP) in the nucleus was established. Using the double-labeled osteosarcoma cells, single cancer-cell seeding in the lung after i.v. injection of osteosarcoma cells was imaged in live mice. Results: The anti-b1 integrin monoclonal antibody, AIIB2, greatly inhibited the seeding of cancer cells on the lung while a control antibody had no effect. To image the efficacy of the anti-integrin antibody on spontaneous metastasis, mice with orthotopically-growing 143B-RFP cells in the tibia were also treated with AIIB2 or control anti-rat IgG1 antibody. After 3 weeks treatment, mice were sacrificed and primary tumors and lung metastases were evaluated with fluorescence imaging. AIIB2 significantly inhibited spontaneous lung metastasis but not primary tumor growth. In a separate experiment, the anti-β1 integrin antibody increased survival in the orthothopic osteosarcoma model. Conclusions: The efficacy of the anti-β1 integrin antibody against metastasis may be due to inhibition of lung seeding of the cancer cells. The increased survival of mice with orthotopically-growing 143B-RFP treated with AIIB2 may be due to inhibition of metastasis, which in turn may be inhibited by effect of the anti-β1 integrin on cancer-cell seeding in the lung.

scholarly journals Drug-Initiated Synthesis of Heterotelechelic Polymer Prodrug Nanoparticles for in Vivo Imaging and Cancer Cell Targeting

2019 ◽  
Vol 20 (7) ◽  
pp. 2464-2476 ◽  
Author(s):  
Daniele Vinciguerra ◽  
Anna Degrassi ◽  
Laura Mancini ◽  
Simona Mura ◽  
Julie Mougin ◽  
...  
Keyword(s):  
Cancer Cell ◽  
In Vivo Imaging ◽  
Cell Targeting ◽  
Cancer Cell Targeting

In Vivo Imaging of Living Cancer Cell and Evaluation for Tissue Atypia Using Endocytoscopy: ECA (Endocytoscopic Atypia) Classification in the Esophagus and Stomach

2008 ◽  
Vol 67 (5) ◽  
pp. AB98 ◽  
Author(s):  
Haruhiro Inoue ◽  
Hitomi Minami ◽  
Hitoshi Satodate ◽  
Makoto Kaga ◽  
Shigeharu Hamatani ◽  
...  
Keyword(s):  
Cancer Cell ◽  
In Vivo Imaging
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