A novel electrochemical biosensor for DNA detection based on exonuclease III-assisted target recycling and rolling circle amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (12) ◽  
pp. 9123-9129 ◽  
Author(s):  
Fenglei Gao ◽  
Yan Du ◽  
Jingwen Yao ◽  
Yanzhuo Zhang ◽  
Jian Gao
Keyword(s):  
Electrochemical Detection ◽  
Electrochemical Biosensor ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Rolling Circle

A strategy for electrochemical detection of DNA by exonuclease III-assisted DNA recycling and the rolling circle amplification was developed.

A ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of the K-ras gene via exonuclease III-assisted target recycling and rolling circle amplification strategies

2019 ◽  
Vol 11 (32) ◽  
pp. 4146-4156 ◽  
Author(s):  
Qi Xiao ◽  
Jinrong Feng ◽  
Jiawen Li ◽  
Yi Liu ◽  
Dan Wang ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Ras Gene ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Rolling Circle ◽  
Exo Iii

A ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of the K-ras gene via Exo III-assisted target recycling and RCA strategies.

A cascade signal amplification strategy for sensitive and label-free DNA detection based on Exo III-catalyzed recycling coupled with rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2884-2889 ◽  
Author(s):  
Xingti Liu ◽  
Qingwang Xue ◽  
Yongshun Ding ◽  
Jing Zhu ◽  
Lei Wang ◽  
...  
Keyword(s):  
Detection Method ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Free Dna ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Cascade Amplification

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.

Rolling circle amplification-mediated in situ synthesis of palladium nanoparticles for the ultrasensitive electrochemical detection of microRNA

The Analyst ◽  
2019 ◽  
Vol 144 (12) ◽  
pp. 3817-3825 ◽  
Author(s):  
Cuiling Zhang ◽  
Dan Li ◽  
Dongwei Li ◽  
Kai Wen ◽  
Xingdong Yang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Palladium Nanoparticles ◽  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
In Situ Synthesis ◽  
Label Free ◽  
Rolling Circle

An ultrasensitive and label-free electrochemical biosensor for microRNA was developed based on rolling circle amplification-mediated palladium nanoparticles.

scholarly journals Rapid electrochemical detection of coronavirus SARS-CoV-2

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Thanyarat Chaibun ◽  
Jiratchaya Puenpa ◽  
Tatchanun Ngamdee ◽  
Nimaradee Boonapatcharoen ◽  
Pornpat Athamanolap ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Sandwich Hybridization ◽  
Redox Active ◽  
One Step ◽  
The One

AbstractCoronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

Sign in / Sign up

Export Citation Format

Share Document