Rolling circle amplification-mediated in situ synthesis of palladium nanoparticles for the ultrasensitive electrochemical detection of microRNA

The Analyst ◽  
2019 ◽  
Vol 144 (12) ◽  
pp. 3817-3825 ◽  
Author(s):  
Cuiling Zhang ◽  
Dan Li ◽  
Dongwei Li ◽  
Kai Wen ◽  
Xingdong Yang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Palladium Nanoparticles ◽  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
In Situ Synthesis ◽  
Label Free ◽  
Rolling Circle

An ultrasensitive and label-free electrochemical biosensor for microRNA was developed based on rolling circle amplification-mediated palladium nanoparticles.

A novel electrochemical biosensor for DNA detection based on exonuclease III-assisted target recycling and rolling circle amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (12) ◽  
pp. 9123-9129 ◽  
Author(s):  
Fenglei Gao ◽  
Yan Du ◽  
Jingwen Yao ◽  
Yanzhuo Zhang ◽  
Jian Gao
Keyword(s):  
Electrochemical Detection ◽  
Electrochemical Biosensor ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Rolling Circle

A strategy for electrochemical detection of DNA by exonuclease III-assisted DNA recycling and the rolling circle amplification was developed.

A functional oligonucleotide probe from an encapsulated silver nanocluster assembled by rolling circle amplification and its application in label-free sensors

RSC Advances ◽  
2016 ◽  
Vol 6 (92) ◽  
pp. 88967-88973 ◽  
Author(s):  
Yuna Guo ◽  
Yu Wang ◽  
Su Liu ◽  
Jinghua Yu ◽  
Qianqian Pei ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Oligonucleotide Probe ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Rolling Circle ◽  
Silver Nanocluster ◽  
E Coli ◽  
Highly Sensitive

A novel label-free, low cost electrochemical biosensor for highly sensitive and selective detection of E. coli has been developed based on rolling circle amplification coupled silver nanoclusters as effective electrochemical probe.

scholarly journals Rapid electrochemical detection of coronavirus SARS-CoV-2

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Thanyarat Chaibun ◽  
Jiratchaya Puenpa ◽  
Tatchanun Ngamdee ◽  
Nimaradee Boonapatcharoen ◽  
Pornpat Athamanolap ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Sandwich Hybridization ◽  
Redox Active ◽  
One Step ◽  
The One

AbstractCoronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

Toehold-initiated Rolling Circle Amplification for Visualizing Individual MicroRNAs In Situ in Single Cells

2014 ◽  
Vol 126 (9) ◽  
pp. 2421-2425 ◽  
Author(s):  
Ruijie Deng ◽  
Longhua Tang ◽  
Qianqian Tian ◽  
Ying Wang ◽  
Lei Lin ◽  
...  
Keyword(s):  
Single Cells ◽  
Rolling Circle Amplification ◽  
Rolling Circle

Sensitive and label-free discrimination of 5-hydroxymethylcytosine and 5-methylcytosine in DNA by ligation-mediated rolling circle amplification

2018 ◽  
Vol 54 (62) ◽  
pp. 8602-8605 ◽  
Author(s):  
Zi-yue Wang ◽  
Meng Wang ◽  
Yan Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Rolling Circle Amplification ◽  
Fluorescence Method ◽  
Label Free ◽  
Rolling Circle

We develop a label-free fluorescence method for sensitive discrimination of 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) by ligation-mediated rolling circle amplification.

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