A Novel Ultrasensitive ECL Sensor for DNA Detection Based on Nicking Endonuclease-Assisted Target Recycling Amplification, Rolling Circle Amplification and Hemin/G-Quadruplex
A new Ag+ detection strategy that can be devised utilizing the advantages of both the nicking endonuclease-assisted signaling amplification (NEASA) method and the label-free architecture of G-quadruplex–hemin complex is reported.
Linear single-labeled DNA probes are used in this RCA-based fluorescence strategy for DNA detection, which could effectively avoid the fluorescence quenching between neighboring signal probes using hairpin probe as signal probe.
A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.
A Novel Ultrasensitive ECL Sensor for DNA Detection Based on Nicking Endonuclease-Assisted Target Recycling Amplification, Rolling Circle Amplification and Hemin/G-Quadruplex