An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells

Lab on a Chip ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 378-381 ◽  
Author(s):  
R. Burger ◽  
D. Kurzbuch ◽  
R. Gorkin ◽  
G. Kijanka ◽  
M. Glynn ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic System ◽  
Fluorescent Detection ◽  
Microfluidic Platform ◽  
Highly Efficient

In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers.

Preprogrammed Microfluidic System for Parallel Anti-reflection Coating by Layer-by-layer Assembly

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Zhenglin Li ◽  
Bongjun Yeom ◽  
Sung-Jin Kim
Keyword(s):  
Microfluidic System ◽  
Coating Process ◽  
Layer By Layer ◽  
Microfluidic Platform ◽  
Lbl Assembly ◽  
Layer By Layer Assembly ◽  
Technology Applications ◽  
Layer Assembly

Layer-by-layer (LbL) assembly is a widely used method of nanofilm coating in various technology applications; however, the coating process is typically time-consuming and labor-intensive. This study presents a microfluidic platform...

scholarly journals On-chip MIC by Combining Concentration Gradient Generator and Flanged Chamber Arrays

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 207 ◽  
Author(s):  
Xiao-Yan Zhang ◽  
Zhe-Yu Li ◽  
Kose Ueno ◽  
Hiroaki Misawa ◽  
Nan-Qi Ren ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Concentration Gradient ◽  
Microfluidic System ◽  
Honeycomb Structure ◽  
Inhibition Test ◽  
Microfluidic Platform ◽  
Initial Concentration ◽  
Bacteria Growth ◽  
Skilled Operator ◽  
On Chip

Minimum inhibition concentration (MIC) of antibiotic is an effective value to ascertain the agent and minimum dosage of inhibiting bacterial growth. However, current techniques to determine MIC are labor intensive and time-consuming, and require skilled operator and high initial concentration of bacteria. To simplify the operation and reduce the time of inhibition test, we developed a microfluidic system, containing a concentration generator and sub-micro-liter chambers, for rapid bacterial growth and inhibition test. To improve the mixing effect, a micropillar array in honeycomb-structure channels is designed, so the steady concentration gradient of amoxicillin can be generated. The flanged chambers are used to culture bacteria under the condition of continuous flow and the medium of chambers is refreshed constantly, which could supply the sufficient nutrient for bacteria growth and take away the metabolite. Based on the microfluidic platform, the bacterial growth with antibiotic inhibition on chip can be quantitatively measured and MIC can be obtained within six hours using low initial concentration of bacteria. Overall, this microfluidic platform has the potential to provide rapidness and effectiveness to screen bacteria and determine MIC of corresponding antibiotics in clinical therapies.

scholarly journals A 3-dimensional microfluidic platform for modeling human extravillous trophoblast invasion and toxicological screening

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yong Pu ◽  
Jeremy Gingrich ◽  
Almudena Veiga-Lopez
Keyword(s):  
Cell Invasion ◽  
Dynamic Environment ◽  
Cell Interaction ◽  
Microfluidic System ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Trophoblast Invasion ◽  
Toxicological Screening ◽  
Microfluidic Platform ◽  
3 Dimensional

A novel 3D microfluidic system for placenta trophoblast cell invasion and cell-to-cell interaction studies under dynamic environment conditions.

Three Dimensional Triangle Chaotic Micromixer

2014 ◽  
Vol 875-877 ◽  
pp. 1189-1193 ◽  
Author(s):  
Lin Li ◽  
Qing De Chen ◽  
C.T. Tsai
Keyword(s):  
Numerical Investigation ◽  
Three Dimensional ◽  
Microfluidic System ◽  
Chaotic Advection ◽  
Mixing Efficiency ◽  
Mixing Index ◽  
Ansys Fluent ◽  
Essential Component ◽  
Highly Efficient

Micromixer is essential component of microfluidic system which has wide application in the field of chemistry and biochemistry. A highly efficient and easily fabricated three dimensional micromixer based on chaotic advection is proposed and investigated. The depth of 25μm for each layer of micromixer and two kinds of fluids, which have viscosities of 0.00097kgm-1s-1and 0.186kgm-1s-1with Re number from 0.001 to 150, are adopted for numerical investigation of mixing efficiency by using ANSYS-Fluent. High mixing index of more than 90% can be obtained by using less than 300μm of length under Re number of 0.01 for mixing Fluid 1. However, it requires 850μm to achieve mixing index of more than 90% for hard-to-mix Fluid 2.

scholarly journals Differential leukocyte counting via fluorescent detection and image processing on a centrifugal microfluidic platform

2016 ◽  
Vol 8 (47) ◽  
pp. 8272-8279 ◽  
Author(s):  
Max L. Balter ◽  
Alvin I. Chen ◽  
C. Amara Colinco ◽  
Alexander Gorshkov ◽  
Brian Bixon ◽  
...  
Keyword(s):  
Image Processing ◽  
Fluorescent Detection ◽  
Microfluidic Platform ◽  
Image Processing Techniques ◽  
Fluorescent Microscope ◽  
Processing Techniques

Methods for enumerating leukocytes on a centrifugal platform using a custom built fluorescent microscope, nuclear stain, and image processing techniques.

Complex Three-Dimensional Fluidic Reservoirs to Control Beads/Living Cells Contacts

2006 ◽  
Author(s):  
Serge Monneret ◽  
Federico Belloni ◽  
Olivier Soppera
Keyword(s):  
Optical Tweezers ◽  
Target Cell ◽  
Three Dimensional ◽  
Microfluidic System ◽  
Optical Traps ◽  
Multiple Point ◽  
Point Interactions ◽  
Cover Glass ◽  
Holographic Optical Tweezers ◽  
Microscope Cover Glass

In this paper, we combine holographic multiple optical tweezers with a three-dimensional microfluidic system to create a versatile microlaboratory. In order to determine cells local and/or temporal response to stimuli, and therefore draw their map of sensitivity, one convenient way is to apply antigen-covered latex beads in order to bind to plasma membrane, by means of optical tweezers. Using multiple optical traps could improve the efficiency of the measurements, but also their versatility. Therefore, we have developed a complete system based on holographic optical tweezers to realise multiple-point interactions between beads and cells with control of the stimulation places, timing, and durations. As we plan to use our system to study biological events in the hour timescale, we have to keep beads and cells separated, in order to prevent unwanted beads to circulate freely in the sample and bind to the target cell during the experiment. We then introduced microstereolithography as a 3D micro-manufacturing approach to the rapid prototyping of three-dimensional fluidic microchambers of complex shapes inside the sample, comprising wells, channels and walls to inject beads locally and keep them separated from cells in our assays. We demonstrated the possibility for microSL to easily and rapidly (typically one hour) fabricate small and three-dimensional observation chambers with customized design of the flow channels, including fluidic reservoirs of typically 500–1500 μm diameter, 5–12 mm height, in order to facilitate manual filling. Several shapes of reservoirs designed to keep beads and cells separated in liquid samples have been realized and successfully tested. Some of them included up to 3 reservoirs, in order to allow co-distribution of different types of beads. Each reservoir typically contained 2–10 μl of solution holding the beads, with a horizontal outlet of 100–200 μm in diameter which allows beads to deposit locally on the microscope cover glass placed under the reservoir outlet. Limited extension of beads under the outlet on the glass has been confirmed, and the ability of the polymeric structures to confine beads in a restricted area has been demonstrated. In the following we present examples of manipulations by multiple holographic optical tweezers consisting at first in extracting several beads from such an area by going through an aperture designed in the structure, making them travel to the target cell, and finally depositing on its outer membrane.

scholarly journals Optical tweezers applied to a microfluidic system

Lab on a Chip ◽  
2004 ◽  
Vol 4 (3) ◽  
pp. 196-200 ◽  
Author(s):  
Jonas Enger ◽  
Mattias Goksör ◽  
Kerstin Ramser ◽  
Petter Hagberg ◽  
Dag Hanstorp
Keyword(s):  
Optical Tweezers ◽  
Microfluidic System

A highly efficient and antifouling microfluidic platform for portable hemodialysis devices

2018 ◽  
Vol 8 (02) ◽  
pp. 474-479 ◽  
Author(s):  
Irfani R. Ausri ◽  
Eliana M. Feygin ◽  
Connie Q. Cheng ◽  
Yuxing Wang ◽  
Zhi Yuan (William) Lin ◽  
...  
Keyword(s):  
Microfluidic Platform ◽  
Highly Efficient ◽  
Image Position

Abstract

Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system

Lab on a Chip ◽  
2018 ◽  
Vol 18 (19) ◽  
pp. 2917-2925 ◽  
Author(s):  
Hong-Lin Cheng ◽  
Chien-Yu Fu ◽  
Wen-Che Kuo ◽  
Yen-Wen Chen ◽  
Yi-Sin Chen ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Cardiovascular Diseases ◽  
Early Detection ◽  
Extracellular Vesicles ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Microfluidic Platform ◽  
System P

A novel microfluidic platform for extracellular vesicle extraction, microRNA isolation and detection with field-effect transistors for early detection of cardiovascular diseases.

Study of a Microfluidic System Based One-Step Blood Cell-Free Region for Biomarker Detection

2019 ◽  
Author(s):  
Anoop Kanjirakat ◽  
Reza Sadr
Keyword(s):  
Blood Cell ◽  
Whole Blood ◽  
Blood Cells ◽  
Numerical Study ◽  
Microfluidic System ◽  
Biomarker Detection ◽  
Microfluidic Platform ◽  
Free Zone ◽  
Free Region ◽  
One Step

Abstract Microfluidic systems are becoming common in the development of point-of-care (PoC) diagnostic systems where various methods are used to efficiently separate blood cells from whole blood. The goal of this research is to develop a passive plasma separator that can easily be integrated into an entire blood-based diagnosis microfluidic platform. In the present work, a one-step process of creating a cell-free region in the flow without the plasma being actively extracted from the whole blood is discussed. Centrifugal force together with a backward facing step is utilized to create a blood cell-free zone. Sensors (typically less than 10 micrometers in size) are proposed to be placed in the cell-free zones for biomarker detection. A detailed numerical study for the design of the microfluidic platform is reported. The two-phase nature of the blood is modeled using a discrete element method (DEM) where blood cells are modeled as spherical constituents with the inclusion of inter-particular interactions. The sizes of the cell-free zones in the microfluidic system are measured for various geometric and flow conditions. An expansion chamber with a larger aspect ratio together with a low Reynold number flow entering it is observed to create a larger cell-free zone.

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