Development of a gold nanoparticle based anti-aggregation method for rapid detection of mercury(ii) in aqueous solutions

2014 ◽  
Vol 6 (15) ◽  
pp. 5690-5696 ◽  
Author(s):  
Genin Gary Huang ◽  
Yen-Ting Chen ◽  
Yu-Rong Lin
Keyword(s):  
Gold Nanoparticles ◽  
Aqueous Solutions ◽  
Gold Nanoparticle ◽  
Rapid Detection ◽  
Aggregation Method

A dynamic anti-aggregation sensing method using unmodified gold nanoparticles(AuNPs) for rapid detection of Hg2+.

Gold nanoparticle-based colorimetric aptasensor for rapid detection of mutiple mycotoxins in rice

2021 ◽  
Author(s):  
Rui Li ◽  
Lin Zhi Li ◽  
Tianzeng Huang ◽  
Xing Liu ◽  
Qi Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Rapid Detection ◽  
Colorimetric Aptasensor

A novel, simple and rapid colorimetric aptasensor for mutiple mycotoxins (Ochratoxin A (OTA) and aflatoxin B1 (AFB1)) detection was developed using unmodified gold nanoparticles (AuNPs). In the work, the high...

scholarly journals A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

RSC Advances ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 1511-1518 ◽  
Author(s):  
Sonu Gandhi ◽  
Ivneet Banga ◽  
Pawan Kumar Maurya ◽  
Sergei A. Eremin
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Single Chain ◽  
Chain Fragment ◽  
Single Chain Fragment Variable ◽  
Single Chain Fragment

Dipstick device developed on the principle of lateral flow using gold nanoparticles for analysis of morphine in urine by morphine/scFv/immunoprobe.

Interference of Gold Nanoparticles with In vitro Endotoxin Detection Assays

2020 ◽  
Vol 16 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Melissa A. Vetten ◽  
Mary Gulumian
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Surface Reactivity ◽  
In Vitro Assays ◽  
Limulus Amebocyte Lysate ◽  
Chromogenic Assay ◽  
Nanoparticle Suspensions ◽  
Endotoxin Contamination ◽  
Factor C

Background: Endotoxin-free engineered nanoparticle suspensions are imperative for their successful applications in the field of nanomedicine as well as in the investigations in their toxicity. Gold nanoparticles are known to interfere with various in vitro assays due to their optical properties and potential for surface reactivity. In vitro endotoxin testing assays are known to be susceptible to interference caused by the sample being tested. Objective: This study aimed to identify a preferred assay for the testing of endotoxin contamination in gold nanoparticle suspensions. Methods: The interference by gold nanoparticles on three assays namely, the commonly used limulus amebocyte lysate chromogenic assay, the limulus amebocyte lysate gel-clot method, and the less common recombinant Factor C (rFC) assay, was tested. Results: Possible interference could be observed with all three assays. The interference with the absorbance- based chromogenic assay could not be overcome by dilution; whilst the qualitative nature of the gel-clot assay excluded the possibility of distinguishing between a false positive result due to enhancement of the sensitivity of the assay, and genuine endotoxin contamination. However, interference with the rFC assay was easily overcome through dilution. Conclusion: The rFC assay is recommended as an option for endotoxin contamination detection in gold nanoparticle suspensions.

scholarly journals CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Nanoparticle Aggregation ◽  
Cleavage Activity ◽  
Gold Nanoparticle Aggregation

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...

Fluorescence biosensor for Salmonella typhimurium detection in food based on nano-self-assembly of alendronic acid modified upconversion and gold nanoparticles

2021 ◽  
Author(s):  
Min Chen ◽  
Leiqing Pan ◽  
K. Tu
Keyword(s):  
Gold Nanoparticles ◽  
Salmonella Typhimurium ◽  
Gold Nanoparticle ◽  
Alendronic Acid ◽  
Self Assembly ◽  
Upconversion Nanoparticles ◽  
Fluorescent Biosensor ◽  
Fluorescence Biosensor

A simple and quick responsive fluorescent biosensor for Salmonella typhimurium detection based on the recognition of aptamer coupled with alendronic acid (ADA)@upconversion nanoparticles (UCNPs) and gold nanoparticle (AuNPs) has been...

scholarly journals Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Metals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 472
Author(s):  
Qunying Yuan ◽  
Manjula Bomma ◽  
Zhigang Xiao
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Synthesis Method ◽  
Nanoparticle Synthesis ◽  
Morphological Properties ◽  
Phytochelatin Synthase ◽  
Rhizobium Tropici ◽  
Whole Cells ◽  
E Coli ◽  
Recombinant Cells

Phytochelatins, the enzymatic products of phytochelatin synthase, play a principal role in protecting the plants from heavy metal and metalloid toxicity due to their ability to scavenge metal ions. In the present study, we investigated the capacity of soluble intracellular extracts from E. coli cells expressing R. tropici phytochelatin synthase to synthesize gold nanoparticle. We discovered that the reaction mediated by soluble extracts from the recombinant E. coli cells had a higher yield of gold nanoparticles, compared to that from the control cells. The compositional and morphological properties of the gold nanoparticles synthesized by the intracellular extracts from recombinant cells and control cells were similar. In addition, this extracellular nanoparticle synthesis method produced purer gold nanoparticles, avoiding the isolation of nanoparticles from cellular debris when whole cells are used to synthesize nanoparticles. Our results suggested that phytochelatins can improve the efficiency of gold nanoparticle synthesis mediated by bacterial soluble intracellular extracts, and the potential of extracellular nanoparticle synthesis platform for the production of nanoparticles in large quantity and pure form is worth further investigation.

scholarly journals Enhanced enzymatic activity from phosphotriesterase trimer gold nanoparticle bioconjugates for pesticide detection

The Analyst ◽  
2017 ◽  
Vol 142 (17) ◽  
pp. 3261-3271 ◽  
Author(s):  
John A. Hondred ◽  
Joyce C. Breger ◽  
Nate T. Garland ◽  
Eunkeu Oh ◽  
Kimihiro Susumu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Enzymatic Activity ◽  
Gold Nanoparticle ◽  
Pesticide Detection

A significant enzymatic enhancement of phosphotriesterase has been demonstrated when immobilized on various sized gold nanoparticles.

Application of nanomaterials in microbial-cell biosensor constructions

2015 ◽  
Vol 69 (1) ◽  
Author(s):  
Jana Šefčovičová ◽  
Jan Tkac
Keyword(s):  
Carbon Nanotubes ◽  
Gold Nanoparticles ◽  
Rapid Detection ◽  
Food Industry ◽  
Low Cost ◽  
Microbial Cell ◽  
Direct Connection ◽  
Qualitative Detection ◽  
Analytical Tools ◽  
Cell Biosensor

AbstractMicrobial cell biosensors, where cells are in direct connection with a transducer enabling quantitative and qualitative detection of an analyte, are very promising analytical tools applied mainly for assays in the environmental field, food industry or biomedicine. Microbial cell biosensors are an excellent alternative to conventional analytical methods due to their specificity, rapid detection and low cost of analysis. Nowadays, nanomaterials are often used in the construction of biosensors to improve their sensitivity and stability. In this review, the combination of microbial and other individual cells with different nanomaterials (carbon nanotubes, graphene, gold nanoparticles, etc.) for the construction of biosensors is described and their applications are provided as well.

Enhanced stability of ZnTPPS by polymeric gold nanoparticles in acidic aqueous solutions

2016 ◽  
Vol 53 (5) ◽  
pp. 270-275 ◽  
Author(s):  
Zhihua Chai ◽  
Liya Ma ◽  
Yanxia Wang ◽  
Xuejun Ren ◽  
Ming Gao
Keyword(s):  
Gold Nanoparticles ◽  
Aqueous Solutions ◽  
Enhanced Stability

Electrodeposition of gold nanoparticles on aryl diazonium monolayer functionalized HOPG surfaces

2016 ◽  
Vol 18 (3) ◽  
pp. 1953-1960 ◽  
Author(s):  
M. C. R. González ◽  
A. G. Orive ◽  
R. C. Salvarezza ◽  
A. H. Creus
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Organic Film

Gold nanoparticle electrodeposition on a modified HOPG surface with a monolayer organic film based on aryl diazonium chemistry has been studied.

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