Gold nanoparticle-based colorimetric aptasensor for rapid detection of mutiple mycotoxins in rice

2021 ◽  
Author(s):  
Rui Li ◽  
Lin Zhi Li ◽  
Tianzeng Huang ◽  
Xing Liu ◽  
Qi Chen ◽  
...  

A novel, simple and rapid colorimetric aptasensor for mutiple mycotoxins (Ochratoxin A (OTA) and aflatoxin B1 (AFB1)) detection was developed using unmodified gold nanoparticles (AuNPs). In the work, the high...

The Analyst ◽  
2015 ◽  
Vol 140 (5) ◽  
pp. 1572-1577 ◽  
Author(s):  
Fen Cheng ◽  
Yue He ◽  
Xiao-Jing Xing ◽  
Dai-Di Tan ◽  
Yi Lin ◽  
...  

A novel strategy for the fabrication of a colorimetric aptasensor using label free gold nanoparticles (AuNPs) is proposed. The aptasensor consists of adenosine (AD) aptamer, AD and AuNPs. The strategy has been employed for the assay of adenosine deaminase (ADA) activity.


2014 ◽  
Vol 6 (15) ◽  
pp. 5690-5696 ◽  
Author(s):  
Genin Gary Huang ◽  
Yen-Ting Chen ◽  
Yu-Rong Lin

A dynamic anti-aggregation sensing method using unmodified gold nanoparticles(AuNPs) for rapid detection of Hg2+.


2015 ◽  
Vol 34 (10) ◽  
pp. 2244-2249 ◽  
Author(s):  
Wenhui Bai ◽  
Chao Zhu ◽  
Jinchuan Liu ◽  
Mengmeng Yan ◽  
Shuming Yang ◽  
...  

2015 ◽  
Vol 7 (3) ◽  
pp. 842-845 ◽  
Author(s):  
Rongping Xiao ◽  
Daifang Wang ◽  
Zhenyu Lin ◽  
Bin Qiu ◽  
Meihua Liu ◽  
...  

We demonstrate the first colorimetric aptasensor for sensitive detection of ochratoxin A based on disassembly of aggregates of oriented AuNP dimers.


2019 ◽  
Vol 287 ◽  
pp. 205-213 ◽  
Author(s):  
Eun-Hee Lee ◽  
Sung Ku Lee ◽  
Min Jung Kim ◽  
Seung-Woo Lee

The Analyst ◽  
2021 ◽  
Author(s):  
Li Zhu ◽  
Yu Zhao ◽  
Shangchen Yao ◽  
Mingzhe Xu ◽  
Lihui Yin ◽  
...  

In this study, a novel colorimetric aptasensor was developed for the rapid detection and visual screening of HPV16 L1 proteins by using the gold nanoparticles (AuNPs) and an RNA aptamer...


RSC Advances ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 1511-1518 ◽  
Author(s):  
Sonu Gandhi ◽  
Ivneet Banga ◽  
Pawan Kumar Maurya ◽  
Sergei A. Eremin

Dipstick device developed on the principle of lateral flow using gold nanoparticles for analysis of morphine in urine by morphine/scFv/immunoprobe.


2020 ◽  
Vol 16 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Melissa A. Vetten ◽  
Mary Gulumian

Background: Endotoxin-free engineered nanoparticle suspensions are imperative for their successful applications in the field of nanomedicine as well as in the investigations in their toxicity. Gold nanoparticles are known to interfere with various in vitro assays due to their optical properties and potential for surface reactivity. In vitro endotoxin testing assays are known to be susceptible to interference caused by the sample being tested. Objective: This study aimed to identify a preferred assay for the testing of endotoxin contamination in gold nanoparticle suspensions. Methods: The interference by gold nanoparticles on three assays namely, the commonly used limulus amebocyte lysate chromogenic assay, the limulus amebocyte lysate gel-clot method, and the less common recombinant Factor C (rFC) assay, was tested. Results: Possible interference could be observed with all three assays. The interference with the absorbance- based chromogenic assay could not be overcome by dilution; whilst the qualitative nature of the gel-clot assay excluded the possibility of distinguishing between a false positive result due to enhancement of the sensitivity of the assay, and genuine endotoxin contamination. However, interference with the rFC assay was easily overcome through dilution. Conclusion: The rFC assay is recommended as an option for endotoxin contamination detection in gold nanoparticle suspensions.


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