MoS2nanosheets as an effective fluorescence quencher for DNA methyltransferase activity detection

The Analyst ◽  
2015 ◽  
Vol 140 (9) ◽  
pp. 3210-3215 ◽  
Author(s):  
Huimin Deng ◽  
Xinjian Yang ◽  
Zhiqiang Gao
Keyword(s):  
Dna Methyltransferase ◽  
Van Der Waals ◽  
Van Der Waals Interactions ◽  
Activity Detection ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Fluorescence Quencher

A fluorometric DNA methyltransferase activity assay is described. MoS2nanosheets are employed as the fluorescence quencherviavan der Waals interactions with fluorophore labeled substrate DNA.

DNA methyltransferase activity assay based on visible light-activated photoelectrochemical biosensor

2014 ◽  
Vol 53 ◽  
pp. 263-267 ◽  
Author(s):  
Yunlei Zhou ◽  
Zhenning Xu ◽  
Mo Wang ◽  
Bing Sun ◽  
Huanshun Yin ◽  
...  
Keyword(s):  
Visible Light ◽  
Dna Methyltransferase ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Photoelectrochemical Biosensor

A sensitive cyclic signal amplification fluorescence strategy for determination of methyltransferase activity based on graphene oxide and RNase H

2019 ◽  
Vol 7 (29) ◽  
pp. 4520-4527 ◽  
Author(s):  
Han Zhao ◽  
Changbei Ma ◽  
Ying Yan ◽  
Mingjian Chen
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Rnase H ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Fluorometric Method

A sensitive fluorometric method for DNA methyltransferase activity detection based on graphene oxide and RNase H-assisted signal amplification.

Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity

2016 ◽  
Vol 52 (12) ◽  
pp. 2517-2520 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Wei Jiang
Keyword(s):  
Dna Methyltransferase ◽  
Detection System ◽  
Rolling Circle Amplification ◽  
Molecular Probe ◽  
Sensitive Assay ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Rolling Circle ◽  
Amplification Strategy ◽  
Fluorescence Detection System

A novel fluorescence detection system is developed for DNA methyltransferase activity assay based on target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy.

DNA methyltransferase activity detection based on graphene quantum dots using fluorescence and fluorescence anisotropy

2017 ◽  
Vol 241 ◽  
pp. 217-223 ◽  
Author(s):  
Hanie Ahmadzade Kermani ◽  
Morteza Hosseini ◽  
Mehdi Dadmehr ◽  
Saman Hosseinkhani ◽  
Mohammad Reza Ganjali
Keyword(s):  
Quantum Dots ◽  
Fluorescence Anisotropy ◽  
Dna Methyltransferase ◽  
Graphene Quantum Dots ◽  
Activity Detection ◽  
Methyltransferase Activity

A glucometer-based strategy for sensitive DNA methyltransferase activity detection via a polymerization nicking reaction and enzyme amplification

2017 ◽  
Vol 9 (20) ◽  
pp. 2933-2938 ◽  
Author(s):  
Ying Chen ◽  
Hongchao Yi
Keyword(s):  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Mtase Activity ◽  
Enzyme Amplification ◽  
Signal Output

The polymerization nicking reaction (PNR) and invertase enzyme amplify the glucometer signal output to achieve simple and sensitive detection of DNA methyltransferase (MTase) activity.

Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

2014 ◽  
Vol 50 (73) ◽  
pp. 10691-10694 ◽  
Author(s):  
Lijuan Ji ◽  
Zhewei Cai ◽  
Yingdan Qian ◽  
Ping Wu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Restriction Endonuclease ◽  
Dna Methyltransferase ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Site Specific ◽  
Inhibitor Screening ◽  
Selective Approach ◽  
Highly Sensitive

A sensitive and selective approach for the DNA methyltransferase activity assay and MTase inhibitor screening is reported.

Antimony selenide/graphene oxide composite for sensitive photoelectrochemical detection of DNA methyltransferase activity

2019 ◽  
Vol 7 (43) ◽  
pp. 6789-6795 ◽  
Author(s):  
Huaijia Xue ◽  
Kaiyang Chen ◽  
Qing Zhou ◽  
Deng Pan ◽  
Yuanjian Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Dna Methyltransferase ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Oxide Composite ◽  
Sandwich Type ◽  
Antimony Selenide

An Sb2Se3/graphene oxide composite was applied as both the photoelectrochemical probe and substrate for biomolecule conjugation for the construction of a “signal-off” sandwich-type biosensor for DNA methyltransferase activity detection.

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