A glucometer-based strategy for sensitive DNA methyltransferase activity detection via a polymerization nicking reaction and enzyme amplification

2017 ◽  
Vol 9 (20) ◽  
pp. 2933-2938 ◽  
Author(s):  
Ying Chen ◽  
Hongchao Yi
Keyword(s):  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Mtase Activity ◽  
Enzyme Amplification ◽  
Signal Output

The polymerization nicking reaction (PNR) and invertase enzyme amplify the glucometer signal output to achieve simple and sensitive detection of DNA methyltransferase (MTase) activity.

Detection of DNA methyltransferase activity using allosteric molecular beacons

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 579-584 ◽  
Author(s):  
Weiting Zhang ◽  
Xiaolong Zu ◽  
Yanling Song ◽  
Zhi Zhu ◽  
Chaoyong James Yang
Keyword(s):  
Dna Methylation ◽  
Dna Methyltransferase ◽  
Molecular Beacon ◽  
Molecular Beacons ◽  
Sensitive Detection ◽  
Methyltransferase Activity ◽  
Mtase Activity ◽  
Adenine Methylation ◽  
Methylation Patterns

Abnormal DNA methylation patterns caused by altered DNA methyltransferase (MTase) activity are closely associated with cancer. Herein, using DNA adenine methylation methyltransferase (Dam MTase) as a model analyte, we designed an allosteric molecular beacon (aMB) for sensitive detection of Dam MTase activity.

Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification

2015 ◽  
Vol 51 (73) ◽  
pp. 13968-13971 ◽  
Author(s):  
Yan Zhang ◽  
Wen-jing Xu ◽  
Ya-ping Zeng ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Methyltransferase Activity

We develop transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification for sensitive detection of DNA methyltransferase activity.

scholarly journals Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase

2018 ◽  
Vol 9 (28) ◽  
pp. 6053-6061 ◽  
Author(s):  
Li-juan Wang ◽  
Xiao Han ◽  
Chen-chen Li ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Specific Detection ◽  
Mtase Activity

Specific and sensitive detection of DNA MTase activity can be achieved by a single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification approach.

A sensitive cyclic signal amplification fluorescence strategy for determination of methyltransferase activity based on graphene oxide and RNase H

2019 ◽  
Vol 7 (29) ◽  
pp. 4520-4527 ◽  
Author(s):  
Han Zhao ◽  
Changbei Ma ◽  
Ying Yan ◽  
Mingjian Chen
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Rnase H ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Fluorometric Method

A sensitive fluorometric method for DNA methyltransferase activity detection based on graphene oxide and RNase H-assisted signal amplification.

DNA methyltransferase activity detection based on graphene quantum dots using fluorescence and fluorescence anisotropy

2017 ◽  
Vol 241 ◽  
pp. 217-223 ◽  
Author(s):  
Hanie Ahmadzade Kermani ◽  
Morteza Hosseini ◽  
Mehdi Dadmehr ◽  
Saman Hosseinkhani ◽  
Mohammad Reza Ganjali
Keyword(s):  
Quantum Dots ◽  
Fluorescence Anisotropy ◽  
Dna Methyltransferase ◽  
Graphene Quantum Dots ◽  
Activity Detection ◽  
Methyltransferase Activity
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