DNA methyltransferase activity detection based on fluorescent silver nanocluster hairpin-shaped DNA probe with 5’-C-rich/G-rich-3’ tails

2015 ◽  
Vol 68 ◽  
pp. 736-740 ◽  
Author(s):  
Wenting Liu ◽  
Han Lai ◽  
Rong Huang ◽  
Chuntao Zhao ◽  
Yimo Wang ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Dna Probe ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Silver Nanocluster

A sensitive cyclic signal amplification fluorescence strategy for determination of methyltransferase activity based on graphene oxide and RNase H

2019 ◽  
Vol 7 (29) ◽  
pp. 4520-4527 ◽  
Author(s):  
Han Zhao ◽  
Changbei Ma ◽  
Ying Yan ◽  
Mingjian Chen
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Rnase H ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Fluorometric Method

A sensitive fluorometric method for DNA methyltransferase activity detection based on graphene oxide and RNase H-assisted signal amplification.

DNA methyltransferase activity detection based on graphene quantum dots using fluorescence and fluorescence anisotropy

2017 ◽  
Vol 241 ◽  
pp. 217-223 ◽  
Author(s):  
Hanie Ahmadzade Kermani ◽  
Morteza Hosseini ◽  
Mehdi Dadmehr ◽  
Saman Hosseinkhani ◽  
Mohammad Reza Ganjali
Keyword(s):  
Quantum Dots ◽  
Fluorescence Anisotropy ◽  
Dna Methyltransferase ◽  
Graphene Quantum Dots ◽  
Activity Detection ◽  
Methyltransferase Activity

A glucometer-based strategy for sensitive DNA methyltransferase activity detection via a polymerization nicking reaction and enzyme amplification

2017 ◽  
Vol 9 (20) ◽  
pp. 2933-2938 ◽  
Author(s):  
Ying Chen ◽  
Hongchao Yi
Keyword(s):  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Mtase Activity ◽  
Enzyme Amplification ◽  
Signal Output

The polymerization nicking reaction (PNR) and invertase enzyme amplify the glucometer signal output to achieve simple and sensitive detection of DNA methyltransferase (MTase) activity.

Antimony selenide/graphene oxide composite for sensitive photoelectrochemical detection of DNA methyltransferase activity

2019 ◽  
Vol 7 (43) ◽  
pp. 6789-6795 ◽  
Author(s):  
Huaijia Xue ◽  
Kaiyang Chen ◽  
Qing Zhou ◽  
Deng Pan ◽  
Yuanjian Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Dna Methyltransferase ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Oxide Composite ◽  
Sandwich Type ◽  
Antimony Selenide

An Sb2Se3/graphene oxide composite was applied as both the photoelectrochemical probe and substrate for biomolecule conjugation for the construction of a “signal-off” sandwich-type biosensor for DNA methyltransferase activity detection.

MoS2nanosheets as an effective fluorescence quencher for DNA methyltransferase activity detection

The Analyst ◽  
2015 ◽  
Vol 140 (9) ◽  
pp. 3210-3215 ◽  
Author(s):  
Huimin Deng ◽  
Xinjian Yang ◽  
Zhiqiang Gao
Keyword(s):  
Dna Methyltransferase ◽  
Van Der Waals ◽  
Van Der Waals Interactions ◽  
Activity Detection ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Fluorescence Quencher

A fluorometric DNA methyltransferase activity assay is described. MoS2nanosheets are employed as the fluorescence quencherviavan der Waals interactions with fluorophore labeled substrate DNA.

scholarly journals Reduced Genomic Cytosine Methylation and Defective Cellular Differentiation in Embryonic Stem Cells Lacking CpG Binding Protein

2005 ◽  
Vol 25 (12) ◽  
pp. 4881-4891 ◽  
Author(s):  
Diana L. Carlone ◽  
Jeong-Heon Lee ◽  
Suzanne R. L. Young ◽  
Erika Dobrota ◽  
Jill Sergesketter Butler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Dna Methyltransferase ◽  
Cellular Differentiation ◽  
Cytosine Methylation ◽  
Epigenetic Modification ◽  
Binding Protein ◽  
Embryonic Stem ◽  
Growth Media ◽  
Methyltransferase Activity

ABSTRACT Cytosine methylation at CpG dinucleotides is a critical epigenetic modification of mammalian genomes. CpG binding protein (CGBP) exhibits a unique DNA-binding specificity for unmethylated CpG motifs and is essential for early murine development. Embryonic stem cell lines deficient for CGBP were generated to further examine CGBP function. CGBP − / − cells are viable but show an increased rate of apoptosis and are unable to achieve in vitro differentiation following removal of leukemia inhibitory factor from the growth media. Instead, CGBP − / − embryonic stem cells remain undifferentiated as revealed by persistent expression of the pluripotent markers Oct4 and alkaline phosphatase. CGBP − / − cells exhibit a 60 to 80% decrease in global cytosine methylation, including hypo-methylation of repetitive elements, single-copy genes, and imprinted genes. Total DNA methyltransferase activity is reduced by 30 to 60% in CGBP − / − cells, and expression of the maintenance DNA methyltransferase 1 protein is similarly reduced. However, de novo DNA methyltransferase activity is normal. Nearly all aspects of the pleiotropic CGBP − / − phenotype are rescued by introduction of a CGBP expression vector. Hence, CGBP is essential for normal epigenetic modification of the genome by cytosine methylation and for cellular differentiation, consistent with the requirement for CGBP during early mammalian development.

scholarly journals DNA methyltransferase activity in the early stages of a sea urchin embryo Evidence of differential control

FEBS Letters ◽  
1995 ◽  
Vol 361 (1) ◽  
pp. 115-117 ◽  
Author(s):  
Luisa Tosi ◽  
Francesco Aniello ◽  
Giuseppe Geraci ◽  
Margherita Branno
Keyword(s):  
Sea Urchin ◽  
Dna Methyltransferase ◽  
Methyltransferase Activity ◽  
Early Stages ◽  
Sea Urchin Embryo ◽  
Differential Control
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