SU-8 bonding protocol for the fabrication of microfluidic devices dedicated to FTIR microspectroscopy of live cells

Lab on a Chip ◽  
2014 ◽  
Vol 14 (1) ◽  
pp. 210-218 ◽  
Author(s):  
Elisa Mitri ◽  
Giovanni Birarda ◽  
Lisa Vaccari ◽  
Saša Kenig ◽  
Massimo Tormen ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Live Cells ◽  
Ftir Microspectroscopy

Synchrotron-FTIR Microspectroscopy Enables the Distinction of Lipid Accumulation in Thraustochytrid Strains Through Analysis of Individual Live Cells

Protist ◽  
2015 ◽  
Vol 166 (1) ◽  
pp. 106-121 ◽  
Author(s):  
Jitraporn Vongsvivut ◽  
Philip Heraud ◽  
Adarsha Gupta ◽  
Tamilselvi Thyagarajan ◽  
Munish Puri ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Live Cells ◽  
Ftir Microspectroscopy

Aberration-free FTIR spectroscopic imaging of live cells in microfluidic devices

The Analyst ◽  
2013 ◽  
Vol 138 (14) ◽  
pp. 4040 ◽  
Author(s):  
K. L. Andrew Chan ◽  
Sergei G. Kazarian
Keyword(s):  
Spectroscopic Imaging ◽  
Microfluidic Devices ◽  
Live Cells

Optical Manipulation of Objects in Microfluidic Devices

2002 ◽  
Vol 729 ◽  
Author(s):  
Erhan Ata ◽  
Aaron L. Birkbeck ◽  
Mihrimah Ozkan ◽  
Cengiz S. Ozkan ◽  
Richard Flynn ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Cell Biology ◽  
Optical Power ◽  
Microfluidic Devices ◽  
Live Cells ◽  
Optical Manipulation ◽  
Polystyrene Spheres ◽  
Pdms Elastomer ◽  
Photon Interactions ◽  
Vcsel Arrays

AbstractIn this paper, we present object manipulation methodologies in microfluidic devices based on object-photon interactions. Devices were fabricated by polydimethylsiloxane (PDMS) elastomer molding of channel structures over photolithographically defined patterns using a thick negative photoresist. Inorganic objects including polystyrene spheres and organic objects including live cells were transferred into fluidic channels using a syringe pump. The objects were trapped and manipulated within the fluidic channels using optical tweezers formed by VCSEL arrays, with only a few mW of optical power. We have also shown that it is possible to manipulate multiple objects as a whole assemble by using an optically-trapped particle as a handle, or an “optical handle”. Optical manipulation will have applications in biomedical devices for drug discovery, cytometry and cell biology research.

scholarly journals Reconfigurable microfluidic circuits for isolating and retrieving cells of interest

2021 ◽  
Author(s):  
Cyril Deroy ◽  
James H R Wheeler ◽  
Agata N Rumianek ◽  
Peter R Cook ◽  
William M Durham ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Diffusion ◽  
Culture Media ◽  
Microfluidic Devices ◽  
Time Lapse ◽  
Live Cells ◽  
Specific Cell ◽  
3D Printed ◽  
Petri Dishes ◽  
Solid Walls

Microfluidic devices are widely used in many fields of biology, but a key limitation is that cells are typically surrounded by solid walls, making it hard to access those that exhibit a specific phenotype for further study. Here, we provide a general and flexible solution to this problem that exploits the remarkable properties of microfluidic circuits with fluid walls - transparent interfaces between culture media and an immiscible fluorocarbon that are easily pierced with pipets. We provide two proofs-of-concept in which specific cell sub-populations are isolated and recovered: i) murine macrophages chemotaxing towards complement component 5a, and ii) bacteria (Pseudomonas aeruginosa) in developing biofilms that migrate towards antibiotics. We build circuits in minutes on standard Petri dishes, add cells, pump in laminar streams so molecular diffusion creates attractant gradients, acquire time-lapse images, and isolate desired sub-populations in real-time by building fluid walls around migrating cells with an accuracy of tens of micrometres using 3D-printed adaptors that convert conventional microscopes into wall-building machines. Our method allows live cells of interest to be easily extracted from microfluidic devices for downstream analyses.

Infrared Microspectroscopy of Live Cells in Microfluidic Devices (MD-IRMS): Toward a Powerful Label-Free Cell-Based Assay

2012 ◽  
Vol 84 (11) ◽  
pp. 4768-4775 ◽  
Author(s):  
L. Vaccari ◽  
G. Birarda ◽  
L. Businaro ◽  
S. Pacor ◽  
G. Grenci
Keyword(s):  
Microfluidic Devices ◽  
Free Cell ◽  
Live Cells ◽  
Label Free ◽  
Infrared Microspectroscopy

scholarly journals Mobile microrobots for bioengineering applications

Lab on a Chip ◽  
2017 ◽  
Vol 17 (10) ◽  
pp. 1705-1724 ◽  
Author(s):  
Hakan Ceylan ◽  
Joshua Giltinan ◽  
Kristen Kozielski ◽  
Metin Sitti
Keyword(s):  
Mobile Robots ◽  
Human Body ◽  
Microfluidic Devices ◽  
Live Cells ◽  
Organ On A Chip

Untethered micron-scale mobile robots can navigate and non-invasively perform specific tasks inside unprecedented and hard-to-reach inner human body sites and inside enclosed organ-on-a-chip microfluidic devices with live cells.

scholarly journals Microfluidic Devices for Terahertz Spectroscopy of Live Cells Toward Lab-on-a-Chip Applications

Sensors ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 476 ◽  
Author(s):  
Qi Tang ◽  
Min Liang ◽  
Yi Lu ◽  
Pak Wong ◽  
Gerald Wilmink ◽  
...  
Keyword(s):  
Terahertz Spectroscopy ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Live Cells

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Sign in / Sign up

Export Citation Format

Share Document