scholarly journals Mobile microrobots for bioengineering applications

Lab on a Chip ◽  
2017 ◽  
Vol 17 (10) ◽  
pp. 1705-1724 ◽  
Author(s):  
Hakan Ceylan ◽  
Joshua Giltinan ◽  
Kristen Kozielski ◽  
Metin Sitti
Keyword(s):  
Mobile Robots ◽  
Human Body ◽  
Microfluidic Devices ◽  
Live Cells ◽  
Organ On A Chip

Untethered micron-scale mobile robots can navigate and non-invasively perform specific tasks inside unprecedented and hard-to-reach inner human body sites and inside enclosed organ-on-a-chip microfluidic devices with live cells.

Intention and Body-Mood Engineering via Proactive Robot Moves in HRI

2015 ◽  
pp. 255-282 ◽  
Author(s):  
O. Can Görür ◽  
Aydan M. Erkmen
Keyword(s):  
Path Planning ◽  
Mobile Robots ◽  
Human Body ◽  
Mood Induction ◽  
Positive Mood ◽  
Focused Attention ◽  
Elastic Networks ◽  
Human Intention ◽  
Novel Concept ◽  
Intention Estimation

This chapter focuses on emotion and intention engineering by socially interacting robots that induce desired emotions/intentions in humans. The authors provide all phases that pave this road, supported by overviews of leading works in the literature. The chapter is partitioned into intention estimation, human body-mood detection through external-focused attention, path planning through mood induction and reshaping intention. Moreover, the authors present their novel concept, with implementation, of reshaping current human intention into a desired one, using contextual motions of mobile robots. Current human intention has to be deviated towards the new desired one by destabilizing the obstinance of human intention, inducing positive mood and making the “robot gain curiosity of human”. Deviations are generated as sequences of transient intentions tracing intention trajectories. The authors use elastic networks to generate, in two modes of body mood: “confident” and “suspicious”, transient intentions directed towards the desired one, choosing among intentional robot moves previously learned by HMM.

SU-8 bonding protocol for the fabrication of microfluidic devices dedicated to FTIR microspectroscopy of live cells

Lab on a Chip ◽  
2014 ◽  
Vol 14 (1) ◽  
pp. 210-218 ◽  
Author(s):  
Elisa Mitri ◽  
Giovanni Birarda ◽  
Lisa Vaccari ◽  
Saša Kenig ◽  
Massimo Tormen ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Live Cells ◽  
Ftir Microspectroscopy

scholarly journals Surface modification of PDMS-based microfluidic devices with collagen using polydopamine as a spacer to enhance primary human bronchial epithelial cell adhesion

2020 ◽  
Author(s):  
Mohammadhossein Dabaghi ◽  
Shadi Shahriari ◽  
Neda Saraei ◽  
Kevin Da ◽  
Abiram Chandiramohan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Surface Modification ◽  
Cell Adhesion ◽  
Microfluidic Devices ◽  
Extracellular Matrix Protein ◽  
Shear Stresses ◽  
Bronchial Epithelial ◽  
Ecm Proteins ◽  
Coating Methods ◽  
Organ On A Chip

AbstractPolydimethylsiloxane (PDMS) is a silicone-based synthetic material that is used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and realizes the fabrication of complicated geometries at the micro and nano scales, it does not optimally interact with cells for adherence and proliferation. Different strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate the complexity of a living tissue microenvironment. For organ-on-a-chip platforms, PDMS surfaces are usually coated by ECM proteins, which occur as a result of physical, weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to find the optimum coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (around three times higher) without showing any discernible difference. These results suggested that such a surface modification can be used to coat an extracellular matrix protein onto PDMS-based microfluidic devices.

Aberration-free FTIR spectroscopic imaging of live cells in microfluidic devices

The Analyst ◽  
2013 ◽  
Vol 138 (14) ◽  
pp. 4040 ◽  
Author(s):  
K. L. Andrew Chan ◽  
Sergei G. Kazarian
Keyword(s):  
Spectroscopic Imaging ◽  
Microfluidic Devices ◽  
Live Cells

scholarly journals Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 132
Author(s):  
Mohammadhossein Dabaghi ◽  
Shadi Shahriari ◽  
Neda Saraei ◽  
Kevin Da ◽  
Abiram Chandiramohan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Surface Modification ◽  
Cell Adhesion ◽  
Cell Attachment ◽  
Microfluidic Devices ◽  
Bronchial Epithelial ◽  
Ecm Proteins ◽  
Coating Methods ◽  
Organ On A Chip

Polydimethylsiloxane (PDMS) is a silicone-based synthetic material used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and assists the fabrication of complicated geometries at micro- and nano-scales, it does not optimally interact with cells for adherence and proliferation. Various strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate a living tissue microenvironment’s complexity. In organ-on-a-chip platforms, PDMS surfaces are usually coated by extracellular matrix (ECM) proteins, which occur as a result of a physical and weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to optimize coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (approximately three times higher) without showing any discernible difference in cell attachment between these two methods. These results suggested that such a surface modification can help coat extracellular matrix protein onto PDMS-based microfluidic devices.

scholarly journals A cost-effective micromilling platform for rapid prototyping of microdevices

TECHNOLOGY ◽  
2016 ◽  
Vol 04 (04) ◽  
pp. 234-239 ◽  
Author(s):  
Daniel P. Yen ◽  
Yuta Ando ◽  
Keyue Shen
Keyword(s):  
Rapid Prototyping ◽  
Low Cost ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Cost Effective ◽  
Multi Scale ◽  
Organ On A Chip

Micromilling has great potential in producing microdevices for lab-on-a-chip and organ-on-a-chip applications, but has remained under-utilized due to the high machinery costs and limited accessibility. In this paper, we assessed the machining capabilities of a low-cost 3-D mill in polycarbonate material, which were showcased by the production of microfluidic devices. The study demonstrates that this particular mill is well suited for the fabrication of multi-scale microdevices with feature sizes from micrometers to centimeters.

Intention and Body-Mood Engineering via Proactive Robot Moves in HRI

2019 ◽  
pp. 247-275
Author(s):  
O. Can Görür ◽  
Aydan M. Erkmen
Keyword(s):  
Path Planning ◽  
Mobile Robots ◽  
Human Body ◽  
Mood Induction ◽  
Positive Mood ◽  
Focused Attention ◽  
Elastic Networks ◽  
Human Intention ◽  
Novel Concept ◽  
Intention Estimation

This chapter focuses on emotion and intention engineering by socially interacting robots that induce desired emotions/intentions in humans. The authors provide all phases that pave this road, supported by overviews of leading works in the literature. The chapter is partitioned into intention estimation, human body-mood detection through external-focused attention, path planning through mood induction and reshaping intention. Moreover, the authors present their novel concept, with implementation, of reshaping current human intention into a desired one, using contextual motions of mobile robots. Current human intention has to be deviated towards the new desired one by destabilizing the obstinance of human intention, inducing positive mood and making the “robot gain curiosity of human”. Deviations are generated as sequences of transient intentions tracing intention trajectories. The authors use elastic networks to generate, in two modes of body mood: “confident” and “suspicious”, transient intentions directed towards the desired one, choosing among intentional robot moves previously learned by HMM.

Optical Manipulation of Objects in Microfluidic Devices

2002 ◽  
Vol 729 ◽  
Author(s):  
Erhan Ata ◽  
Aaron L. Birkbeck ◽  
Mihrimah Ozkan ◽  
Cengiz S. Ozkan ◽  
Richard Flynn ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Cell Biology ◽  
Optical Power ◽  
Microfluidic Devices ◽  
Live Cells ◽  
Optical Manipulation ◽  
Polystyrene Spheres ◽  
Pdms Elastomer ◽  
Photon Interactions ◽  
Vcsel Arrays

AbstractIn this paper, we present object manipulation methodologies in microfluidic devices based on object-photon interactions. Devices were fabricated by polydimethylsiloxane (PDMS) elastomer molding of channel structures over photolithographically defined patterns using a thick negative photoresist. Inorganic objects including polystyrene spheres and organic objects including live cells were transferred into fluidic channels using a syringe pump. The objects were trapped and manipulated within the fluidic channels using optical tweezers formed by VCSEL arrays, with only a few mW of optical power. We have also shown that it is possible to manipulate multiple objects as a whole assemble by using an optically-trapped particle as a handle, or an “optical handle”. Optical manipulation will have applications in biomedical devices for drug discovery, cytometry and cell biology research.

scholarly journals Rapid Manufacturing of Multilayered Microfluidic Devices for Organ on a Chip Applications

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1382
Author(s):  
Roberto Paoli ◽  
Davide Di Giuseppe ◽  
Maider Badiola-Mateos ◽  
Eugenio Martinelli ◽  
Maria Jose Lopez-Martinez ◽  
...  
Keyword(s):  
Rapid Prototyping ◽  
Soft Lithography ◽  
Microfluidic Devices ◽  
Device Fabrication ◽  
Rapid Manufacturing ◽  
Digital Manufacturing ◽  
Proof Of Concept ◽  
Cyclic Olefin ◽  
Protocol Optimization ◽  
Organ On A Chip

Microfabrication and Polydimethylsiloxane (PDMS) soft-lithography techniques became popular for microfluidic prototyping at the lab, but even after protocol optimization, fabrication is yet a long, laborious process and partly user-dependent. Furthermore, the time and money required for the master fabrication process, necessary at any design upgrade, is still elevated. Digital Manufacturing (DM) and Rapid-Prototyping (RP) for microfluidics applications arise as a solution to this and other limitations of photo and soft-lithography fabrication techniques. Particularly for this paper, we will focus on the use of subtractive DM techniques for Organ-on-a-Chip (OoC) applications. Main available thermoplastics for microfluidics are suggested as material choices for device fabrication. The aim of this review is to explore DM and RP technologies for fabrication of an OoC with an embedded membrane after the evaluation of the main limitations of PDMS soft-lithography strategy. Different material options are also reviewed, as well as various bonding strategies. Finally, a new functional OoC device is showed, defining protocols for its fabrication in Cyclic Olefin Polymer (COP) using two different RP technologies. Different cells are seeded in both sides of the membrane as a proof of concept to test the optical and fluidic properties of the device.

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