scholarly journals Microfluidic Devices for Terahertz Spectroscopy of Live Cells Toward Lab-on-a-Chip Applications

Sensors ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 476 ◽  
Author(s):  
Qi Tang ◽  
Min Liang ◽  
Yi Lu ◽  
Pak Wong ◽  
Gerald Wilmink ◽  
...  
Keyword(s):  
Terahertz Spectroscopy ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Live Cells

scholarly journals Emerging Lab-on-a-Chip Approaches for Liquid Biopsy in Lung Cancer: Status in CTCs and ctDNA Research and Clinical Validation

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2101
Author(s):  
Ângela Carvalho ◽  
Gabriela Ferreira ◽  
Duarte Seixas ◽  
Catarina Guimarães-Teixeira ◽  
Rui Henrique ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Liquid Biopsy ◽  
Residual Disease ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Early Recurrence ◽  
Circulating Tumor Dna ◽  
Clinical Validation ◽  
Liquid Biopsies ◽  
Lung Cancer Patients

Despite the intensive efforts dedicated to cancer diagnosis and treatment, lung cancer (LCa) remains the leading cause of cancer-related mortality, worldwide. The poor survival rate among lung cancer patients commonly results from diagnosis at late-stage, limitations in characterizing tumor heterogeneity and the lack of non-invasive tools for detection of residual disease and early recurrence. Henceforth, research on liquid biopsies has been increasingly devoted to overcoming these major limitations and improving management of LCa patients. Liquid biopsy is an emerging field that has evolved significantly in recent years due its minimally invasive nature and potential to assess various disease biomarkers. Several strategies for characterization of circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) have been developed. With the aim of standardizing diagnostic and follow-up practices, microfluidic devices have been introduced to improve biomarkers isolation efficiency and specificity. Nonetheless, implementation of lab-on-a-chip platforms in clinical practice may face some challenges, considering its recent application to liquid biopsies. In this review, recent advances and strategies for the use of liquid biopsies in LCa management are discussed, focusing on high-throughput microfluidic devices applied for CTCs and ctDNA isolation and detection, current clinical validation studies and potential clinical utility.

scholarly journals Recent Advances in Continuous-Flow Particle Manipulations Using Magnetic Fluids

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 744 ◽  
Author(s):  
Xiangchun Xuan
Keyword(s):  
Continuous Flow ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Magnetic Fluids ◽  
Label Free ◽  
Particle Manipulation ◽  
The Past ◽  
Recent Advances ◽  
Medium Exchange ◽  
Flow Particle

Magnetic field-induced particle manipulation is simple and economic as compared to other techniques (e.g., electric, acoustic, and optical) for lab-on-a-chip applications. However, traditional magnetic controls require the particles to be manipulated being magnetizable, which renders it necessary to magnetically label particles that are almost exclusively diamagnetic in nature. In the past decade, magnetic fluids including paramagnetic solutions and ferrofluids have been increasingly used in microfluidic devices to implement label-free manipulations of various types of particles (both synthetic and biological). We review herein the recent advances in this field with focus upon the continuous-flow particle manipulations. Specifically, we review the reported studies on the negative magnetophoresis-induced deflection, focusing, enrichment, separation, and medium exchange of diamagnetic particles in the continuous flow of magnetic fluids through microchannels.

Manufacturing Issues and Considerations in Thermal Bonding of Polymer Based Lab-on-a-Chip

2009 ◽  
Vol 74 ◽  
pp. 175-178
Author(s):  
Yu Xin Koh ◽  
Sum Huan Ng ◽  
Khin Thet May ◽  
Cong Zhi Chan ◽  
Zhi Ping Wang
Keyword(s):  
Bond Strength ◽  
Surface Topography ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Thermal Bonding ◽  
Bonding Pressure ◽  
Bonding Quality ◽  
Viscoelastic Effect ◽  
Common Technique

The sealing of microchannels is a key step in the fabrication of microfluidic devices and thermal bonding is a common technique used. Here, major manufacturing issues and considerations in thermal bonding are investigated, including bonding quality and microchannel deformations. Flatness of substrate is extremely crucial to the uniformity in bonding. While increased bonding pressure helps to overcome problems related to surface topography and to enhance bond strength, its significant impact on geometrical changes of microchannel due to viscoelastic effect should also be taken into consideration.

A Microfluidic Mixer Fabricated From Compliant Thermoplastic Films

2008 ◽  
Vol 2 (1) ◽  
Author(s):  
N. Paya ◽  
T. Dankovic ◽  
A. Feinerman
Keyword(s):  
Reynolds Number ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Reynolds Numbers ◽  
Mixing Efficiency ◽  
Minimal Amount ◽  
Microfluidic Mixer ◽  
Mixing Performance ◽  
Rapid Mixing ◽  
Microfluidic Flows

Mixing is often crucial to the operation of various microfluidic devices. And the most common objective is rapid mixing between two initially segregated fluid streams in a minimal amount of space. In microfluidic flows characterized by incompressibility and low Reynolds number, however, turbulence is almost entirely absent and mixing generally relies on diffusion. Therefore, based on the properties of the fluids involved, it can take impractically long to achieve high mixing efficiency in some cases. To resolve this problem, this paper demonstrates a novel compliant micromixer made of thermoplastic films for lab-on-a-chip applications. The microfluidic mixer utilizes self-rotation effects to achieve high mixing efficiency at Reynolds numbers below 100. In addition, a possible design is suggested for a thermoplastic voltage-actuated micromixer which can lead to even better mixing performance at Reynolds numbers below 1.

EXPERIMENTAL INVESTIGATIONS ON THE INTERACTIONS BETWEEN LIQUIDS AND STRUCTURES TO PASSIVELY CONTROL THE SURFACE-DRIVEN CAPILLARY FLOW IN MICROFLUIDIC LAB-ON-A-CHIP SYSTEMS TO SEPARATE THE MICROPARTICLES FOR BIOENGINEERING APPLICATIONS

2016 ◽  
Vol 24 (06) ◽  
pp. 1750075 ◽  
Author(s):  
SUBHADEEP MUKHOPADHYAY
Keyword(s):  
Surface Area ◽  
Capillary Flow ◽  
Separation Efficiency ◽  
Volume Ratio ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Research Paper ◽  
Novel Approach ◽  
The Individual ◽  
Effect Of Surface

In this research paper, total 246 individual microfluidic devices have been fabricated by maskless lithography, hot embossing lithography and direct bonding technique. The effect of surface area to volume ratio on the surface-driven capillary flow of different liquids has been experimentally investigated in these microfluidic devices fabricated by polymethylmethacrylate (PMMA). Also, the individual effects of liquid viscosity and surface wettability on the surface-driven capillary flow of different liquids are experimentally investigated. The polystyrene particles of 10[Formula: see text][Formula: see text]m diameters have been separated from the aqueous microparticle suspensions in the microfluidic lab-on-a-chip systems with 100% separation efficiency. Also, the polystyrene particles of 5[Formula: see text][Formula: see text]m diameters have been separated from a different set of aqueous microparticle suspensions in the microfluidic lab-on-a-chip systems with 100% separation efficiency. The individual designs of the microfluidic lab-on-a-chip systems are a novel approach in this research paper. The effect of surface area to volume ratio on the separation time is experimentally investigated as another novel approach of this research paper.

SU-8 bonding protocol for the fabrication of microfluidic devices dedicated to FTIR microspectroscopy of live cells

Lab on a Chip ◽  
2014 ◽  
Vol 14 (1) ◽  
pp. 210-218 ◽  
Author(s):  
Elisa Mitri ◽  
Giovanni Birarda ◽  
Lisa Vaccari ◽  
Saša Kenig ◽  
Massimo Tormen ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Live Cells ◽  
Ftir Microspectroscopy

scholarly journals Elasto-Magnetic Pumps Integrated within Microfluidic Devices

2021 ◽  
Vol 4 (1) ◽  
pp. 48
Author(s):  
Jacob L. Binsley ◽  
Elizabeth L. Martin ◽  
Thomas O. Myers ◽  
Stefano Pagliara ◽  
Feodor Y. Ogrin
Keyword(s):  
Magnetic System ◽  
Point Of Care ◽  
Microfluidic Channel ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Point Of Care Testing ◽  
Ongoing Research ◽  
Flow Rates ◽  
Pumping System ◽  
Oscillating Magnetic Field

Many lab-on-a-chip devices require a connection to an external pumping system in order to perform their function. While this is not problematic in typical laboratory environments, it is not always practical when applied to point-of-care testing, which is best utilized outside of the laboratory. Therefore, there has been a large amount of ongoing research into producing integrated microfluidic components capable of generating effective fluid flow from on-board the device. This research aims to introduce a system that can produce practical flow rates, and be easily fabricated and actuated using readily available techniques and materials. We show how an asymmetric elasto-magnetic system, inspired by Purcell’s three-link swimmer, can provide this solution through the generation of non-reciprocal motion in an enclosed environment. The device is fabricated monolithically within a microfluidic channel at the time of manufacture, and is actuated using a weak, oscillating magnetic field. The flow rate can be altered dynamically, and the direction of the resultant flow can be controlled by adjusting the frequency of the driving field. The device has been proven, experimentally and numerically, to operate effectively when applied to fluids with a range of viscosities. Such a device may be able to replace external pumping systems in portable applications.

Optimized short digestion protocol for free fetal DNA detection using methylation-dependent markers

2017 ◽  
Vol 41 (4) ◽  
Author(s):  
Ana B. Rodríguez-Martínez ◽  
Esther Sarasola-Díez ◽  
Estíbaliz Achalandabaso ◽  
María J. García-Barcina
Keyword(s):  
Dna Sequences ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Restriction Enzymes ◽  
Circulating Free Dna ◽  
Free Dna ◽  
Chip Technology ◽  
Long Duration ◽  
Important Diagnostic Tool ◽  
Rate Of Success

AbstractBackground:Circulating free DNA (cfDNA) digestion with methylation sensitive restriction enzymes constitutes an important diagnostic tool for differentiating methylated from non-methylated DNA sequences. In the context of pregnancy, this is used to differentiate fetal from maternal DNA. Current protocols are of long duration and use multiple enzymes with different incubation and inactivating temperatures. We describe a short protocol for the digestion of circulating free DNA focused on its future adaptation to miniaturized microfluidic devices based on lab-on-a-chip technology.Methods:cfDNA was extracted from plasma samples of pregnant and non-pregnant women with chemagic Viral NA/gDNA and QIAamp circulating nucleic acids kits. For digestion protocol optimization, different methylation sensitive and insensitive restriction enzymes were used. Detection ofResults:The digestion protocol is optimized to a 3.5 h one-step protocol using the enzymes BstUI, BstY1 and HhaI resulting in a complete digestion of the hypomethylated maternalConclusions:This work provides a digestion protocol for cfDNA samples with a combination of temperatures (37 °C and 60 °C) and a protocol length (<4 h) which facilitates its adaptation to miniaturized microfluidic devices based on lab-on-a-chip technology. In this technology, the shorter the duration of the protocol, the greater the rate of success and the less sample evaporation.

scholarly journals PDMS-Based Microdevices for the Capture of MicroRNA Biomarkers

2020 ◽  
Vol 10 (11) ◽  
pp. 3867 ◽  
Author(s):  
Lorenzo Lunelli ◽  
Federica Barbaresco ◽  
Giorgio Scordo ◽  
Cristina Potrich ◽  
Lia Vanzetti ◽  
...  
Keyword(s):  
Working Conditions ◽  
Liquid Biopsy ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Extensive Study ◽  
Small Cell ◽  
Main Concern ◽  
Circulating Biomarkers ◽  
Small Cell Lung ◽  
Proof Of Principle

The isolation and analysis of circulating biomarkers, the main concern of liquid biopsy, could greatly benefit from microfluidics. Microfluidics has indeed the huge potentiality to bring liquid biopsy into the clinical practice. Here, two polydimethylsiloxane (PDMS)-based microdevices are presented as valid tools for capturing microRNAs biomarkers from clinically-relevant samples. After an extensive study of functionalized polydimethylsiloxane (PDMS) properties in adsorbing/eluting microRNAs, the best conditions were transferred to the microdevices, which were thoroughly characterized. The channels morphology and chemical composition were measured, and parameters for the automation of measures were setup. The best working conditions were then used with microdevices, which were proven to capture microRNAs on all channel surfaces. Finally, microfluidic devices were successfully validated via real-time PCR for the detection of a pool of microRNAs related to non-small cell lung cancer, selected as proof-of-principle. The microfluidic approach described here will allow a step forward towards the realization of an efficient microdevice, possibly automated and integrated into a microfluidic lab-on-a-chip with high analytical potentialities.

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