Interdigitated array of Pt electrodes for electrical stimulation and engineering of aligned muscle tissue

Lab on a Chip ◽  
2012 ◽  
Vol 12 (18) ◽  
pp. 3491 ◽  
Author(s):  
Samad Ahadian ◽  
Javier Ramón-Azcón ◽  
Serge Ostrovidov ◽  
Gulden Camci-Unal ◽  
Vahid Hosseini ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Pt Electrodes ◽  
Interdigitated Array

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

An ink-jet printed electrical stimulation platform for muscle tissue regeneration

Bioprinting ◽  
2018 ◽  
Vol 11 ◽  
pp. e00035 ◽  
Author(s):  
Gabriele Maria Fortunato ◽  
Carmelo De Maria ◽  
David Eglin ◽  
Tiziano Serra ◽  
Giovanni Vozzi
Keyword(s):  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Tissue Regeneration ◽  
Ink Jet

Electrical stimulation with Pt electrodes. V. The effect of protein on Pt dissolution

Biomaterials ◽  
1980 ◽  
Vol 1 (3) ◽  
pp. 135-139 ◽  
Author(s):  
L.S. Robblee ◽  
J. McHardy ◽  
J.M. Marston ◽  
S.B. Brummer
Keyword(s):  
Electrical Stimulation ◽  
Pt Electrodes ◽  
Pt Dissolution

scholarly journals Malleability of skeletal muscle in overcoming limitations: structural elements

1985 ◽  
Vol 115 (1) ◽  
pp. 355-364 ◽  
Author(s):  
H. Hoppeler ◽  
S. L. Lindstedt
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Unit Volume ◽  
Volume Density ◽  
Skeletal Muscle Tissue ◽  
African Mammals ◽  
Wide Range ◽  
Chronic Electrical Stimulation

The quantitative structural composition of skeletal muscle tissue shows a wide range of variability among different species of animals and in any one species among muscles with a different function. Moreover, experimental manipulations such as exercise training or chronic electrical stimulation can dramatically change the ultrastructural appearance of the muscles involved. Both in endurance exercise and in chronic electrical stimulation the volume density of mitochondria can be increased greatly (by more than three-fold in the stimulation experiments). This happens without an apparent change of the internal architecture of the mitochondria, since the surface density of the inner mitochondrial membranes remains constant. In situations where both the mitochondrial volume and the maximal rate of oxygen consumption of the muscle tissue are known, these two variables are found to be linearly related. It can be calculated that the ‘maximal’ oxygen consumption of a unit volume of mitochondria in muscle is close to 5 ml O2 min-1 cm-3 under comparable conditions in man, mouse and a series of African mammals. It is hypothesized that there is a constant volume of oxygen metabolized per unit volume of mitochondria and unit time under limiting conditions in working skeletal muscle tissue. Given the efficiency of muscular energy conversion, this would allow an estimate of the potential for aerobic power production of a muscle from measurement of its volume density of mitochondria.

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