The high functionalization of temperature-responsive culture dishes for establishing advanced cell sheet engineering

2010 ◽  
Vol 20 (40) ◽  
pp. 8768 ◽  
Author(s):  
Kazuyoshi Itoga ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive

scholarly journals Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering

2009 ◽  
Vol 6 (suppl_3) ◽  
Author(s):  
Kenichi Nagase ◽  
Jun Kobayashi ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Copolymer Composition ◽  
Cell Sheets ◽  
Chromatographic Separations ◽  
Cell Sheet Engineering ◽  
External Temperature ◽  
Cell Monolayers ◽  
Temperature Responsive ◽  
Confluent Cell ◽  
Dependent Cell

Temperature-responsive intelligent surfaces, prepared by the modification of an interface with poly( N -isopropylacrylamide) and its derivatives, have been used for biomedical applications. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated interactions with biomolecules and cells. In this review, we focus on the application of these intelligent surfaces to chromatographic separation and cell cultures. Chromatographic separations using several types of intelligent surfaces are mentioned briefly, and various effects related to the separation of bioactive compounds are discussed, including wettability, copolymer composition and graft polymer architecture. Similarly, we also summarize temperature-responsive cell culture substrates that allow the recovery of confluent cell monolayers as contiguous living cell sheets for tissue-engineering applications. The key factors in temperature-dependent cell adhesion/detachment control are discussed from the viewpoint of grafting temperature-responsive polymers, and new methodologies for effective cell sheet culturing and the construction of thick tissues are summarized.

scholarly journals Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

Polymers ◽  
2012 ◽  
Vol 4 (3) ◽  
pp. 1478-1498 ◽  
Author(s):  
Zhonglan Tang ◽  
Yoshikatsu Akiyama ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Polymer ◽  
Modified Surface

scholarly journals Design of Temperature-Responsive Cell Culture Surfaces for Cell Sheet Engineering

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Y. Akiyama
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Cell Sheet ◽  
Extensive Study ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Interdisciplinary Field ◽  
Culture Surface ◽  
Responsive Cell

Temperature-responsive cell culture surfaces, which modulate cell attachment/detachment characteristics with temperature, have been used to fabricate cell sheets. Extensive study on fabrication of cell sheet with the temperature-responsive cell culture surface, manipulation, and transplantation of the cell sheet has established the interdisciplinary field of cell sheet engineering, in which engineering, biological, and medical fields closely collaborate. Such collaboration has pioneered cell sheet engineering, making it a promising and attractive technology in tissue engineering and regenerative medicine. This review introduces concepts of cell sheet engineering, followed by designs for the fabrication of various types of temperature-responsive cell culture surfaces and technologies for cell sheet manipulation. The development of various methods for the fabrication of temperature-responsive cell culture surfaces was also summarized. The availability of cell sheet engineering for the treatment and regeneration of damaged human tissue has also been described, providing examples of the clinical application of cell sheet transplantation in humans.

Temperature-responsive cell culture surfaces for regenerative medicine with cell sheet engineering

2007 ◽  
Vol 32 (8-9) ◽  
pp. 1123-1133 ◽  
Author(s):  
Masayuki Yamato ◽  
Yoshikatsu Akiyama ◽  
Jun Kobayashi ◽  
Joseph Yang ◽  
Akihiko Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Cell

Nanotechnology-Based Cell Sheet Engineering for Regenerative Medicine

2006 ◽  
Vol 53 ◽  
pp. 74-78
Author(s):  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Regenerative Medicine ◽  
Immobilized Cells ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Intelligent Materials ◽  
Responsive Polymers ◽  
Temperature Responsive ◽  
Novel Technology ◽  
Temperature Responsive Polymers

In order to realize true regenerative medicine, we have developed a novel technology for the reconstruction of tissues and organs by utilizing intelligent materials including temperature-responsive polymers. We developed temperature-responsive culture surfaces, on which temperature-responsive polymers are covalently immobilized. Cells are cultured on the surfaces at 37°C, and harvested as transplantable cell sheets by reducing temperature to 20°C. With these cell sheets we regenerate various kinds of tissues such as cornea and heart.

Thermo-Intelligent Surfaces for Cell Culture

2006 ◽  
Vol 53 ◽  
pp. 70-73
Author(s):  
Teruo Okano
Keyword(s):  
Intact Cell ◽  
Single Cells ◽  
Cell Sheet ◽  
Cell Junctions ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Responsive Polymers ◽  
Temperature Responsive ◽  
Biodegradable Scaffolds ◽  
Tissue Reconstruction

In order to avoid several complications resulted from biodegradable scaffolds or single cell injection, we have developed “cell sheet engineering”. Our concept is novel tissue reconstruction not from single cells but from cell sheets. In order to prepare cell sheets, temperature-responsive culture dishes are utilized. Since temperature-responsive polymers are covalently grafted on the dishes, various types of cells adhere and proliferate on them at 37°C, but are spontaneously detached only by reducing temperature below 32°C without any need for proteolytic enzyme. All the confluent cells are noninvasively harvested as a single contiguous cell sheets with intact cell-cell junctions and deposited extracellular matrix. We have utilized these harvested cell sheets for various tissue reconstructions including ocular surfaces, periodontal ligament, cardiac patches as well as bladder.

Mussel adhesive protein inspired coatings on temperature-responsive hydrogels for cell sheet engineering

2016 ◽  
Vol 4 (36) ◽  
pp. 6012-6022 ◽  
Author(s):  
Yu Bin Lee ◽  
Young Min Shin ◽  
Eun Mi Kim ◽  
Joong-yup Lee ◽  
Jangsoo Lim ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Adhesion Properties ◽  
Adhesive Protein ◽  
Temperature Responsive ◽  
Mussel Adhesive Protein ◽  
A Cell ◽  
Mussel Adhesive ◽  
Responsive Hydrogels

A cell sheet translocation system is developed based on a temperature-responsive hydrogel with modular cell adhesion properties by a mussel-inspired polydopamine coating.

Cell-Sheet Engineering Using Intelligent Surfaces

MRS Bulletin ◽  
2005 ◽  
Vol 30 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Joseph Yang ◽  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Non Invasive ◽  
Temperature Responsive ◽  
Temperature Reduction ◽  
Novel Technology ◽  
Responsive Polymer

AbstractThe possibility of recreating various tissues and organs for the purpose of regenerative medicine has received much interest. However, the field of tissue engineering has been restricted by the limitations of conventional approaches. A method to circumvent the need for traditional scaffold-based technologies is cell-sheet engineering, which uses temperature-responsive culture dishes. These surfaces, which are created by grafting the temperature-responsive polymer poly(N-isopropylacrylamide) onto ordinary culture dishes, enable the non-invasive harvesting of cells as intact sheets by simple temperature reduction. This article reviews current research on the applications of cell-sheet engineering for the reconstruction of various tissues, as well as the intelligent surfaces used by this novel technology.

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