Multilayered mouse preosteoblast MC3T3-E1 sheets harvested from temperature-responsive poly(N-isopropylacrylamide-co-acrylamide) grafted culture surface for cell sheet engineering

2013 ◽  
Vol 129 (5) ◽  
pp. 3061-3069 ◽  
Author(s):  
Sopita Wong-In ◽  
Nguyen Thi KhanhThuyen ◽  
Wanwipa Siriwatwechakul ◽  
Kwanchanok Viravaidya-Pasuwat
Keyword(s):  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Culture Surface

scholarly journals Design of Temperature-Responsive Cell Culture Surfaces for Cell Sheet Engineering

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Y. Akiyama
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Cell Sheet ◽  
Extensive Study ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Interdisciplinary Field ◽  
Culture Surface ◽  
Responsive Cell

Temperature-responsive cell culture surfaces, which modulate cell attachment/detachment characteristics with temperature, have been used to fabricate cell sheets. Extensive study on fabrication of cell sheet with the temperature-responsive cell culture surface, manipulation, and transplantation of the cell sheet has established the interdisciplinary field of cell sheet engineering, in which engineering, biological, and medical fields closely collaborate. Such collaboration has pioneered cell sheet engineering, making it a promising and attractive technology in tissue engineering and regenerative medicine. This review introduces concepts of cell sheet engineering, followed by designs for the fabrication of various types of temperature-responsive cell culture surfaces and technologies for cell sheet manipulation. The development of various methods for the fabrication of temperature-responsive cell culture surfaces was also summarized. The availability of cell sheet engineering for the treatment and regeneration of damaged human tissue has also been described, providing examples of the clinical application of cell sheet transplantation in humans.

Human Amniotic Cell Sheet Harvest Using a Novel Temperature-Responsive Culture Surface Coated with Protein-Based Polymer

2006 ◽  
Vol 12 (2) ◽  
pp. 391-401 ◽  
Author(s):  
Helin Zhang ◽  
Masamichi Iwama ◽  
Toshihiro Akaike ◽  
Dan W. Urry ◽  
Asima Pattanaik ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Temperature Responsive ◽  
Amniotic Cell ◽  
Culture Surface

scholarly journals Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering

2009 ◽  
Vol 6 (suppl_3) ◽  
Author(s):  
Kenichi Nagase ◽  
Jun Kobayashi ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Copolymer Composition ◽  
Cell Sheets ◽  
Chromatographic Separations ◽  
Cell Sheet Engineering ◽  
External Temperature ◽  
Cell Monolayers ◽  
Temperature Responsive ◽  
Confluent Cell ◽  
Dependent Cell

Temperature-responsive intelligent surfaces, prepared by the modification of an interface with poly( N -isopropylacrylamide) and its derivatives, have been used for biomedical applications. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated interactions with biomolecules and cells. In this review, we focus on the application of these intelligent surfaces to chromatographic separation and cell cultures. Chromatographic separations using several types of intelligent surfaces are mentioned briefly, and various effects related to the separation of bioactive compounds are discussed, including wettability, copolymer composition and graft polymer architecture. Similarly, we also summarize temperature-responsive cell culture substrates that allow the recovery of confluent cell monolayers as contiguous living cell sheets for tissue-engineering applications. The key factors in temperature-dependent cell adhesion/detachment control are discussed from the viewpoint of grafting temperature-responsive polymers, and new methodologies for effective cell sheet culturing and the construction of thick tissues are summarized.

Human Amniotic Cell Sheet Harvest Using a Novel Temperature-Responsive Culture Surface Coated with Protein-Based Polymer

2006 ◽  
Vol 0 (0) ◽  
pp. 060224120000002
Author(s):  
Helin Zhang ◽  
Masamichi Iwama ◽  
Toshihiro Akaike ◽  
Dan W. Urry ◽  
Asima Pattanaik ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Temperature Responsive ◽  
Amniotic Cell ◽  
Culture Surface

scholarly journals Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

Polymers ◽  
2012 ◽  
Vol 4 (3) ◽  
pp. 1478-1498 ◽  
Author(s):  
Zhonglan Tang ◽  
Yoshikatsu Akiyama ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Polymer ◽  
Modified Surface

Temperature-responsive cell culture surfaces for regenerative medicine with cell sheet engineering

2007 ◽  
Vol 32 (8-9) ◽  
pp. 1123-1133 ◽  
Author(s):  
Masayuki Yamato ◽  
Yoshikatsu Akiyama ◽  
Jun Kobayashi ◽  
Joseph Yang ◽  
Akihiko Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Cell

Nanotechnology-Based Cell Sheet Engineering for Regenerative Medicine

2006 ◽  
Vol 53 ◽  
pp. 74-78
Author(s):  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Regenerative Medicine ◽  
Immobilized Cells ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Intelligent Materials ◽  
Responsive Polymers ◽  
Temperature Responsive ◽  
Novel Technology ◽  
Temperature Responsive Polymers

In order to realize true regenerative medicine, we have developed a novel technology for the reconstruction of tissues and organs by utilizing intelligent materials including temperature-responsive polymers. We developed temperature-responsive culture surfaces, on which temperature-responsive polymers are covalently immobilized. Cells are cultured on the surfaces at 37°C, and harvested as transplantable cell sheets by reducing temperature to 20°C. With these cell sheets we regenerate various kinds of tissues such as cornea and heart.

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