Mussel adhesive protein inspired coatings on temperature-responsive hydrogels for cell sheet engineering

2016 ◽  
Vol 4 (36) ◽  
pp. 6012-6022 ◽  
Author(s):  
Yu Bin Lee ◽  
Young Min Shin ◽  
Eun Mi Kim ◽  
Joong-yup Lee ◽  
Jangsoo Lim ◽  
...  

A cell sheet translocation system is developed based on a temperature-responsive hydrogel with modular cell adhesion properties by a mussel-inspired polydopamine coating.

2005 ◽  
Vol 897 ◽  
Author(s):  
Anand Doraiswamy ◽  
R J Narayan ◽  
C Z Dinu ◽  
R Cristescu ◽  
P B Messersmith ◽  
...  

AbstractMussel adhesive protein analogs are biologically-derived materials that possess unique adhesion properties. We have demonstrated thin film growth of DOPA modified- PEG block copolymer mussel adhesive protein analogs using a novel laser processing technique known as matrix assisted pulsed laser evaporation (MAPLE). The main functional groups of the mussel adhesive protein analog are present in the MAPLE-transferred film. These novel polymer thin films have numerous medical, electronic, and marine applications.


Biomaterials ◽  
2007 ◽  
Vol 28 (28) ◽  
pp. 4039-4046 ◽  
Author(s):  
Dong Soo Hwang ◽  
Sung Bo Sim ◽  
Hyung Joon Cha

2018 ◽  
Vol 6 (24) ◽  
pp. 4151-4163 ◽  
Author(s):  
Dongchuan Yang ◽  
Juhui Qiu ◽  
Ning Xu ◽  
Yinping Zhao ◽  
Tianhan Li ◽  
...  

A bioactive material based on mussel adhesive protein Mfp-5 fused with VE-cadherin extracellular domain specifically enhances the adhesion of endothelial cells.


2007 ◽  
Vol 127 (4) ◽  
pp. 727-735 ◽  
Author(s):  
Dong Soo Hwang ◽  
Youngsoo Gim ◽  
Dong Gyun Kang ◽  
Yeon Kyu Kim ◽  
Hyung Joon Cha

2008 ◽  
Vol 47-50 ◽  
pp. 861-864
Author(s):  
Dong Soo Hwang ◽  
Seong Hye Lim ◽  
Hyung Joon Cha

Mussel foot protein is widely known for candidate of strong adhesive materials. Six times fp-1 decapeptide repeats and fp-5 are chosen among the various type of foot protein and fused into (MAP) fp-151. Through various cell-adhesion analyses, we previously demonstrated that fp- 151 has the potential to be used as a cell or tissue bioadhesive. In the present study, to improve the cell-adhesion properties of fp-151, it was designed a new cell-adhesive protein, fp-151-RGD, which is a fusion with the GRGDSP residues, a RGD peptide sequence that has previously been identified at the cell-attachment site of fibronectin, at the C-terminus of fp-151. Also, recombinant fp-151- RGD maintained the advantages associated with fp-151, such as a high production yield in Escherichia coli and simple purification, it showed superior spreading ability, which is important for cell proliferation under serum-free conditions, as well as better cell-adhesion ability compared with other commercially produced cell-adhesion materials such as poly-L-lysine (PLL) and the naturally extracted MAP mixture Cell-Tak. The excellent adhesion and spreading abilities of fp- 151-RGD might be due to the fact that it utilizes three types of cell-binding mechanisms: DOPA adhesion of Cell-Tak, cationic binding force of PLL, and RGD sequence-mediated adhesion of fibronectin. Therefore, the new recombinant fp-151-RGD is suitable for use as a cell-adhesion material in cell culture or tissue engineering, and in any other area where efficient cell adhesion is required.


Biomaterials ◽  
1992 ◽  
Vol 13 (14) ◽  
pp. 1000-1008 ◽  
Author(s):  
Marion P. Olivieri ◽  
Robert E. Baier ◽  
Ronald E. Loomis

2016 ◽  
Vol 70 ◽  
pp. 260-264 ◽  
Author(s):  
Young Hoon Song ◽  
Jeong Hyun Seo ◽  
Yoo Seong Choi ◽  
Do Hyung Kim ◽  
Bong-Hyuk Choi ◽  
...  

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