Cell-Sheet Engineering Using Intelligent Surfaces

MRS Bulletin ◽  
2005 ◽  
Vol 30 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Joseph Yang ◽  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Non Invasive ◽  
Temperature Responsive ◽  
Temperature Reduction ◽  
Novel Technology ◽  
Responsive Polymer

AbstractThe possibility of recreating various tissues and organs for the purpose of regenerative medicine has received much interest. However, the field of tissue engineering has been restricted by the limitations of conventional approaches. A method to circumvent the need for traditional scaffold-based technologies is cell-sheet engineering, which uses temperature-responsive culture dishes. These surfaces, which are created by grafting the temperature-responsive polymer poly(N-isopropylacrylamide) onto ordinary culture dishes, enable the non-invasive harvesting of cells as intact sheets by simple temperature reduction. This article reviews current research on the applications of cell-sheet engineering for the reconstruction of various tissues, as well as the intelligent surfaces used by this novel technology.

Nanotechnology-Based Cell Sheet Engineering for Regenerative Medicine

2006 ◽  
Vol 53 ◽  
pp. 74-78
Author(s):  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Regenerative Medicine ◽  
Immobilized Cells ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Intelligent Materials ◽  
Responsive Polymers ◽  
Temperature Responsive ◽  
Novel Technology ◽  
Temperature Responsive Polymers

In order to realize true regenerative medicine, we have developed a novel technology for the reconstruction of tissues and organs by utilizing intelligent materials including temperature-responsive polymers. We developed temperature-responsive culture surfaces, on which temperature-responsive polymers are covalently immobilized. Cells are cultured on the surfaces at 37°C, and harvested as transplantable cell sheets by reducing temperature to 20°C. With these cell sheets we regenerate various kinds of tissues such as cornea and heart.

scholarly journals Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

Polymers ◽  
2012 ◽  
Vol 4 (3) ◽  
pp. 1478-1498 ◽  
Author(s):  
Zhonglan Tang ◽  
Yoshikatsu Akiyama ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Polymer ◽  
Modified Surface

Temperature-responsive cell culture surfaces for regenerative medicine with cell sheet engineering

2007 ◽  
Vol 32 (8-9) ◽  
pp. 1123-1133 ◽  
Author(s):  
Masayuki Yamato ◽  
Yoshikatsu Akiyama ◽  
Jun Kobayashi ◽  
Joseph Yang ◽  
Akihiko Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Cell

scholarly journals Regenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces

2013 ◽  
Vol 58 (35) ◽  
pp. 4349-4356 ◽  
Author(s):  
Terumasa Umemoto ◽  
Masayuki Yamato ◽  
Kohji Nishida ◽  
Teruo Okano
Keyword(s):  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive

scholarly journals Recent Advances in ROS-Responsive Cell Sheet Techniques for Tissue Engineering

2019 ◽  
Vol 20 (22) ◽  
pp. 5656 ◽  
Author(s):  
Min-Ah Koo ◽  
Mi Hee Lee ◽  
Jong-Chul Park
Keyword(s):  
Tissue Engineering ◽  
Cell Sheet ◽  
Cell Detachment ◽  
Oxygen Species ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
New Approach ◽  
Responsive Systems ◽  
Cell Based Therapy ◽  
Responsive Cell

Cell sheet engineering has evolved rapidly in recent years as a new approach for cell-based therapy. Cell sheet harvest technology is important for producing viable, transplantable cell sheets and applying them to tissue engineering. To date, most cell sheet studies use thermo-responsive systems to detach cell sheets. However, other approaches have been reported. This review provides the progress in cell sheet detachment techniques, particularly reactive oxygen species (ROS)-responsive strategies. Therefore, we present a comprehensive introduction to ROS, their application in regenerative medicine, and considerations on how to use ROS in cell detachment. The review also discusses current limitations and challenges for clarifying the mechanism of the ROS-responsive cell sheet detachment.

Cell delivery in regenerative medicine: The cell sheet engineering approach

2006 ◽  
Vol 116 (2) ◽  
pp. 193-203 ◽  
Author(s):  
Joseph Yang ◽  
Masayuki Yamato ◽  
Kohji Nishida ◽  
Takeshi Ohki ◽  
Masato Kanzaki ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Delivery ◽  
Cell Sheet Engineering ◽  
Engineering Approach
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