scholarly journals Long-term high-resolution imaging and culture of C. elegans in chip-gel hybrid microfluidic device for developmental studies

Lab on a Chip ◽  
2010 ◽  
Vol 10 (14) ◽  
pp. 1862 ◽  
Author(s):  
Jan Krajniak ◽  
Hang Lu
Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1424-1431 ◽  
Author(s):  
Guillaume Aubry ◽  
Mei Zhan ◽  
Hang Lu

We present a microfluidic device for high-resolution imaging and sorting of early larval C. elegans. The animals are isolated in droplets and temporarily immobilized for imaging using a reversible hydrogel.


2017 ◽  
Vol 40 (2) ◽  
pp. 202-214 ◽  
Author(s):  
Wolfgang Keil ◽  
Lena M. Kutscher ◽  
Shai Shaham ◽  
Eric D. Siggia

2018 ◽  
Vol 259 ◽  
pp. 703-708 ◽  
Author(s):  
Liang Huang ◽  
Peng Zhao ◽  
Junhan Wu ◽  
Han-Sheng Chuang ◽  
Wenhui Wang

Lab on a Chip ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 1802-1802
Author(s):  
Simon Berger ◽  
Evelyn Lattmann ◽  
Tinri Aegerter-Wilmsen ◽  
Michael Hengartner ◽  
Alex Hajnal ◽  
...  

Correction for ‘Long-term C. elegans immobilization enables high resolution developmental studies in vivo’ by Simon Berger et al., Lab Chip, 2018, 18, 1359–1368.


Lab on a Chip ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 1359-1368 ◽  
Author(s):  
Simon Berger ◽  
Evelyn Lattmann ◽  
Tinri Aegerter-Wilmsen ◽  
Michael Hengartner ◽  
Alex Hajnal ◽  
...  

Microfluidics enables the interference free observation of sensitive developmental processes in C. elegans.


Retina ◽  
2012 ◽  
Vol 32 (2) ◽  
pp. 375-386 ◽  
Author(s):  
Jeffrey K. Luttrull ◽  
Christopher Sramek ◽  
Daniel Palanker ◽  
Charles J. Spink ◽  
David C. Musch

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2312-2317 ◽  
Author(s):  
Fanghao Hu ◽  
Lu Wei ◽  
Chaogu Zheng ◽  
Yihui Shen ◽  
Wei Min

High-resolution imaging of choline metabolites in living mammalian cells, primary neurons andC. eleganshas been demonstrated with the potential forin vivodisease detection and developmental monitoring.


2008 ◽  
Vol 2008 (2) ◽  
pp. pdb.prot4902-pdb.prot4902 ◽  
Author(s):  
B. E. Chen ◽  
J. T. Trachtenberg ◽  
A. J.G.D. Holtmaat ◽  
K. Svoboda

2009 ◽  
Vol 17 (6) ◽  
pp. 16-19 ◽  
Author(s):  
B. Cline ◽  
R. Luo ◽  
K. Kuhlmann

Many infectious diseases prevalent in the developing world, including malaria and tuberculosis, are difficult to diagnose on the basis of symptoms alone but can be accurately detected using microscope examination. Currently the expense, size, and fragility of optical microscopes impede their widespread use in resource-limited settings. Addressing these obstacles facing microscopy in the developing world is a pressing need; over 800,000 people, primarily children in Africa, die annually of malaria, and more than 1,500,000 people die annually of tuberculosis [1][2]. The aim of this study is to design and validate a microscope for use in the developing world that combines high-resolution imaging, extreme affordability, and long-term durability.


Author(s):  
Holger Fehlauer ◽  
Adam L. Nekimken ◽  
Anna A. Kim ◽  
Beth L. Pruitt ◽  
Miriam B. Goodman ◽  
...  

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