scholarly journals Long-Term High-Resolution Imaging of Developing C. elegans Larvae with Microfluidics

2017 ◽  
Vol 40 (2) ◽  
pp. 202-214 ◽  
Author(s):  
Wolfgang Keil ◽  
Lena M. Kutscher ◽  
Shai Shaham ◽  
Eric D. Siggia
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
C Elegans ◽  
Resolution Imaging

On-demand dielectrophoretic immobilization and high-resolution imaging of C. elegans in microfluids

2018 ◽  
Vol 259 ◽  
pp. 703-708 ◽  
Author(s):  
Liang Huang ◽  
Peng Zhao ◽  
Junhan Wu ◽  
Han-Sheng Chuang ◽  
Wenhui Wang
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
C Elegans ◽  
On Demand ◽  
Resolution Imaging

LONG-TERM SAFETY, HIGH-RESOLUTION IMAGING, AND TISSUE TEMPERATURE MODELING OF SUBVISIBLE DIODE MICROPULSE PHOTOCOAGULATION FOR RETINOVASCULAR MACULAR EDEMA

Retina ◽  
2012 ◽  
Vol 32 (2) ◽  
pp. 375-386 ◽  
Author(s):  
Jeffrey K. Luttrull ◽  
Christopher Sramek ◽  
Daniel Palanker ◽  
Charles J. Spink ◽  
David C. Musch
Keyword(s):  
High Resolution ◽  
Macular Edema ◽  
Tissue Temperature ◽  
High Resolution Imaging ◽  
Temperature Modeling ◽  
Resolution Imaging

scholarly journals Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2312-2317 ◽  
Author(s):  
Fanghao Hu ◽  
Lu Wei ◽  
Chaogu Zheng ◽  
Yihui Shen ◽  
Wei Min
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Mammalian Cells ◽  
Stimulated Raman Scattering ◽  
Primary Neurons ◽  
High Resolution Imaging ◽  
Metabolic Labeling ◽  
C Elegans ◽  
Resolution Imaging

High-resolution imaging of choline metabolites in living mammalian cells, primary neurons andC. eleganshas been demonstrated with the potential forin vivodisease detection and developmental monitoring.

Long-Term, High-Resolution Imaging in the Neocortex In Vivo

2008 ◽  
Vol 2008 (2) ◽  
pp. pdb.prot4902-pdb.prot4902 ◽  
Author(s):  
B. E. Chen ◽  
J. T. Trachtenberg ◽  
A. J.G.D. Holtmaat ◽  
K. Svoboda
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Resolution Imaging

Design and Validation of a Low-Cost Microscope for Diagnostics in the Developing World

2009 ◽  
Vol 17 (6) ◽  
pp. 16-19 ◽  
Author(s):  
B. Cline ◽  
R. Luo ◽  
K. Kuhlmann
Keyword(s):  
High Resolution ◽  
Infectious Diseases ◽  
Developing World ◽  
Low Cost ◽  
High Resolution Imaging ◽  
Microscope Examination ◽  
Resource Limited Settings ◽  
Resource Limited ◽  
Resolution Imaging

Many infectious diseases prevalent in the developing world, including malaria and tuberculosis, are difficult to diagnose on the basis of symptoms alone but can be accurately detected using microscope examination. Currently the expense, size, and fragility of optical microscopes impede their widespread use in resource-limited settings. Addressing these obstacles facing microscopy in the developing world is a pressing need; over 800,000 people, primarily children in Africa, die annually of malaria, and more than 1,500,000 people die annually of tuberculosis [1][2]. The aim of this study is to design and validate a microscope for use in the developing world that combines high-resolution imaging, extreme affordability, and long-term durability.

scholarly journals Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

10.3791/56530 ◽  
2018 ◽  
Author(s):  
Holger Fehlauer ◽  
Adam L. Nekimken ◽  
Anna A. Kim ◽  
Beth L. Pruitt ◽  
Miriam B. Goodman ◽  
...  
Keyword(s):  
High Resolution ◽  
Mechanical Stimulation ◽  
High Resolution Imaging ◽  
C Elegans ◽  
Resolution Imaging

scholarly journals Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans

Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1424-1431 ◽  
Author(s):  
Guillaume Aubry ◽  
Mei Zhan ◽  
Hang Lu
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Microfluidic Device ◽  
High Resolution Imaging ◽  
Microfluidic Platform ◽  
C Elegans ◽  
Resolution Imaging

We present a microfluidic device for high-resolution imaging and sorting of early larval C. elegans. The animals are isolated in droplets and temporarily immobilized for imaging using a reversible hydrogel.

scholarly journals Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 1116-1127 ◽  
Author(s):  
Adam L. Nekimken ◽  
Holger Fehlauer ◽  
Anna A. Kim ◽  
Sandra N. Manosalvas-Kjono ◽  
Purim Ladpli ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Neuronal Activity ◽  
High Resolution Imaging ◽  
C Elegans ◽  
Resolution Imaging ◽  
Simultaneous Immobilization ◽  
Stimulation Of

A new microfluidic tool for simultaneous immobilization, force delivery and high resolution imaging of neuronal activity in living Caenorhabditis elegans.

scholarly journals 3D-printed microplate inserts for long term high-resolution imaging of live brain organoids

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Mariana Oksdath Mansilla ◽  
Camilo Salazar-Hernandez ◽  
Sally L. Perrin ◽  
Kaitlin G. Scheer ◽  
Gökhan Cildir ◽  
...  
Keyword(s):  
High Resolution ◽  
Cell Tracking ◽  
Culture Conditions ◽  
Significant Advance ◽  
High Resolution Imaging ◽  
Whole Brain ◽  
3D Printed ◽  
Resolution Imaging ◽  
Brain Organoid

Abstract Background Organoids are a reliable model used in the study of human brain development and under pathological conditions. However, current methods for brain organoid culture generate tissues that range from 0.5 to 2 mm of size, which need to be constantly agitated to allow proper oxygenation. The culture conditions are, therefore, not suitable for whole-brain organoid live imaging, required to study developmental processes and disease progression within physiologically relevant time frames (i.e. days, weeks, months). Results Here we designed 3D-printed microplate inserts adaptable to standard 24 multi-well plates, which allow the growth of multiple organoids in pre-defined and fixed XYZ coordinates. This innovation facilitates high-resolution imaging of whole-cerebral organoids, allowing precise assessment of organoid growth and morphology, as well as cell tracking within the organoids, over long periods. We applied this technology to track neocortex development through neuronal progenitors in brain organoids, as well as the movement of patient-derived glioblastoma stem cells within healthy brain organoids. Conclusions This new bioengineering platform constitutes a significant advance that permits long term detailed analysis of whole-brain organoids using multimodal inverted fluorescence microscopy.

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