Pre-storage of liquid reagents in glass ampoules for DNA extraction on a fully integrated lab-on-a-chip cartridge

Lab on a Chip ◽  
2010 ◽  
Vol 10 (11) ◽  
pp. 1480 ◽  
Author(s):  
Jochen Hoffmann ◽  
Daniel Mark ◽  
Sascha Lutz ◽  
Roland Zengerle ◽  
Felix von Stetten
Keyword(s):  
Dna Extraction ◽  
Lab On A Chip ◽  
Fully Integrated

A fully integrated passive microfluidic Lab-on-a-Chip for real-time electrochemical detection of ammonium: Sewage applications

2019 ◽  
Vol 653 ◽  
pp. 1223-1230 ◽  
Author(s):  
J. Gallardo-Gonzalez ◽  
A. Baraket ◽  
S. Boudjaoui ◽  
T. Metzner ◽  
F. Hauser ◽  
...  
Keyword(s):  
Real Time ◽  
Electrochemical Detection ◽  
Lab On A Chip ◽  
Fully Integrated

On-Chip Platinum Micro-Heater with Platinum Temperature Sensor for a Fully Integrated Disposable PCR Module

2010 ◽  
Vol 93-94 ◽  
pp. 129-132 ◽  
Author(s):  
W. Sripumkhai ◽  
A. Lekwichai ◽  
Win Bunjongpru ◽  
S. Porntheeraphat ◽  
B. Tunhoo ◽  
...  
Keyword(s):  
Thermal Cycling ◽  
Temperature Sensor ◽  
Lab On A Chip ◽  
Conventional Pcr ◽  
Fully Integrated ◽  
On Chip

The on-chip platinum micro-heater prototypes for thermal cycling equipped with platinum temperature sensor are fabricated. The device has been designed, fabricated and characterized to explore the feasibility of the micro-heater for a fully integrated disposable lab-on-a-chip with the PCR module. The on-chip micro-heater demonstrates that the temperature transitions are shorter by comparison with the conventional PCR temperature routines.

scholarly journals Towards a Fully Integrated Lab-On-A-Chip

2018 ◽  
Vol 13 (1) ◽  
pp. 28-31
Author(s):  
Joerg Nestler ◽  
Harald Peter ◽  
Frank Bier
Keyword(s):  
Lab On A Chip ◽  
Fully Integrated

scholarly journals A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 537 ◽  
Author(s):  
Cancan Zhu ◽  
Anzhong Hu ◽  
Junsheng Cui ◽  
Ke Yang ◽  
Xinchao Zhu ◽  
...  
Keyword(s):  
High Risk ◽  
Dna Extraction ◽  
Molecular Diagnostics ◽  
Solid Phase ◽  
Lab On A Chip ◽  
Clinical Diagnostics ◽  
Oligonucleotide Array ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Multiplex Amplification

Point-of-care (POC) molecular diagnostics play a crucial role in the prevention and treatment of infectious diseases. It is necessary to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. In this study, we proposed a lab-on-a-chip device that integrated DNA extraction, solid-phase PCR and genotyping detection. The ingenious design of the pneumatic microvalves enabled the fluid mixing and reagent storage to be organically combined, significantly reducing the size of the chip. The solid oligonucleotide array incorporated into the chip allowed the spatial separation of the primers and minimized undesirable interactions in multiplex amplification. As a proof-of-concept for POC molecular diagnostics on the device, five genotypes of high-risk human papillomavirus (HPV) (HPV16/HPV18/HPV31/HPV33/HPV58) were examined. Positive quality control samples and HPV patient cervical swab specimens were analyzed on the integrated microdevice. The platform was capable of detection approximately 50 copies of HPV virus per reaction during a single step, including DNA extraction, solid-phase PCR and genotype detection, in 1 h from samples being added to the chip. This simple and inexpensive microdevice provided great utility for the screening and monitoring of HPV genotypes. The sample-to-result platform will pave the way for wider application of POC molecular testing in the fields of clinical diagnostics, food safety, and environmental monitoring.

A Lab-on-a-Chip for Clinical Analysis with Acoustic Microagitation based on Piezoelectric Poly(Vinilidene Fluoride)

2008 ◽  
Vol 1138 ◽  
Author(s):  
P. Martins ◽  
V. Cardoso ◽  
J. Serrado Nunes ◽  
L. Rebouta ◽  
G. Rocha ◽  
...  
Keyword(s):  
Vinylidene Fluoride ◽  
Biological Fluids ◽  
Point Of Care ◽  
Lab On A Chip ◽  
Beta Phase ◽  
Clinical Analysis ◽  
Fully Integrated ◽  
Quantitative Measurements ◽  
Novel Approach ◽  
Poly Vinylidene Fluoride

AbstractThis paper reports on the incorporation and validation of a microagitation system based on a piezoelectric polymer, Poly(vinylidene fluoride) in its beta phase, β-PVDF, in a fully-integrated disposable lab-on-a-chip for point-of-care testing and monitoring of biochemical parameters in biological fluids. The lab-on-a-chip concept offers a novel approach for clinical analyses, especially in biological fluids analyses, due to its portability, ensuring that the analysis can be performed at any location with quick results. Its microagitation system performance was successfully demonstrated by quantitative measurements of uric acid in human urine, though other molecules or biological fluids can be also measured. The optimization tests prove that it is possible to use lower frequencies than resonance with no major changes in the mixing process. The effect of area and location within the lab-on-a-chip of the microagitation system was also considered.

Ultrasonic Transducer Based on β-PVDF for Fluidic Microagitation in a Lab-on-a-Chip Device

2008 ◽  
Vol 57 ◽  
pp. 99-104 ◽  
Author(s):  
Vanessa F. Cardoso ◽  
P. Martins ◽  
Jivago Serrado-Nunes ◽  
L. Rebouta ◽  
José Gerardo Rocha ◽  
...  
Keyword(s):  
Reaction Rate ◽  
Biological Fluids ◽  
Ultrasonic Transducer ◽  
Lab On A Chip ◽  
Reaction Chamber ◽  
Electronic Control ◽  
Fully Integrated ◽  
Piezoelectric Polymer ◽  
Transmittance Curve ◽  
Transparent Conductive Electrodes

This paper describes a fully-integrated lab-on-a-chip device for testing and monitoring biochemical parameters in biological fluids. The major innovation of this microdevice is the application of an acoustic microagitation technique with automatic electronic control based on a β-PVDF piezoelectric polymer placed underneath the microfluidic structures. Experimental results regarding the influence of the thickness of the polymer on the reaction rate of biological fluids are presented. Moreover, the study of the transmittance curve of β-PVDF with transparent conductive electrodes is also presented. Transparent electrodes are a constraint once the polymer is incorporate underneath the reaction chamber due to the analytical measurement by spectrophotometry.

Fully integrated optical systems for lab-on-a-chip applications

2005 ◽  
Author(s):  
Soren Balslev ◽  
Brian Bilenberg ◽  
Daniel Nilsson ◽  
Anders M. Jorgensen ◽  
Anders Kristensen ◽  
...  
Keyword(s):  
Lab On A Chip ◽  
Optical Systems ◽  
Fully Integrated ◽  
Integrated Optical
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