Vacuum supported liquid waste handling for dna extraction on centrifugally operated Lab-on-a-Chip systems

2009 ◽  
Author(s):  
D. Mark ◽  
S. Haeberle ◽  
S. Lutz ◽  
R. Zengerle ◽  
F. von Stetten
Keyword(s):  
Dna Extraction ◽  
Liquid Waste ◽  
Lab On A Chip ◽  
Waste Handling

scholarly journals A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 537 ◽  
Author(s):  
Cancan Zhu ◽  
Anzhong Hu ◽  
Junsheng Cui ◽  
Ke Yang ◽  
Xinchao Zhu ◽  
...  
Keyword(s):  
High Risk ◽  
Dna Extraction ◽  
Molecular Diagnostics ◽  
Solid Phase ◽  
Lab On A Chip ◽  
Clinical Diagnostics ◽  
Oligonucleotide Array ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Multiplex Amplification

Point-of-care (POC) molecular diagnostics play a crucial role in the prevention and treatment of infectious diseases. It is necessary to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. In this study, we proposed a lab-on-a-chip device that integrated DNA extraction, solid-phase PCR and genotyping detection. The ingenious design of the pneumatic microvalves enabled the fluid mixing and reagent storage to be organically combined, significantly reducing the size of the chip. The solid oligonucleotide array incorporated into the chip allowed the spatial separation of the primers and minimized undesirable interactions in multiplex amplification. As a proof-of-concept for POC molecular diagnostics on the device, five genotypes of high-risk human papillomavirus (HPV) (HPV16/HPV18/HPV31/HPV33/HPV58) were examined. Positive quality control samples and HPV patient cervical swab specimens were analyzed on the integrated microdevice. The platform was capable of detection approximately 50 copies of HPV virus per reaction during a single step, including DNA extraction, solid-phase PCR and genotype detection, in 1 h from samples being added to the chip. This simple and inexpensive microdevice provided great utility for the screening and monitoring of HPV genotypes. The sample-to-result platform will pave the way for wider application of POC molecular testing in the fields of clinical diagnostics, food safety, and environmental monitoring.

scholarly journals Assessment of Liquid Waste Handling Practice of Addis Ketema and its Impact on Shankela River, Addis Ababa, Ethiopia

2022 ◽  
Vol 6 (1) ◽  
pp. em0097
Author(s):  
Solomon Tibebu ◽  
Abebe Worku ◽  
Abebaw Shebeshi ◽  
Estifanos Kassahun
Keyword(s):  
Addis Ababa ◽  
Liquid Waste ◽  
Waste Handling

scholarly journals The liquid waste/slurry of swine farms, ecological problem of environmental contamination and its control

2007 ◽  
Vol 23 (3-4) ◽  
pp. 109-118
Author(s):  
Dobrila Jakic-Dimic ◽  
I. Pavlovic ◽  
B. Savic
Keyword(s):  
Environmental Contamination ◽  
Liquid Waste ◽  
Quality Characteristic ◽  
Ecological Problem ◽  
Swine Farms ◽  
Waste Handling

The liquid waste because its quantitative and quality characteristic, way of prepare, possible epidemiological consequences, raw and odor smell presence a great ecological problem. Invest to development to handling waste considered separately and resolve of technological problems are minimize because we misunderstand its start and finishing phases and its overall. In farms process of waste handling suppose secondary and separate of breeding process. Reasons to separation was economical because production generate acquisition and wasting consumption it. .

A lab-on-a-chip for free-flow electrophoretic preconcentration of viruses and gel electrophoretic DNA extraction

The Analyst ◽  
2020 ◽  
Vol 145 (7) ◽  
pp. 2554-2561
Author(s):  
Matthias Hügle ◽  
Ole Behrmann ◽  
Madlen Raum ◽  
Frank T. Hufert ◽  
Gerald A. Urban ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Lab On A Chip ◽  
Free Flow

A lab-on-a-chip for FFE preconcentration of viruses and gel electrophoretic DNA extraction: complete preparation of amplifiable DNA from dilute specimens.

Pre-storage of liquid reagents in glass ampoules for DNA extraction on a fully integrated lab-on-a-chip cartridge

Lab on a Chip ◽  
2010 ◽  
Vol 10 (11) ◽  
pp. 1480 ◽  
Author(s):  
Jochen Hoffmann ◽  
Daniel Mark ◽  
Sascha Lutz ◽  
Roland Zengerle ◽  
Felix von Stetten
Keyword(s):  
Dna Extraction ◽  
Lab On A Chip ◽  
Fully Integrated
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