scholarly journals Automated live cell imaging of cell migration across a microfluidic-controlled chemoattractant gradient

2015 ◽  
Vol 12 (11) ◽  
pp. vii-viii ◽  
Author(s):  
Philip Lee ◽  
Cindy S Y Chen ◽  
Terry Gaige ◽  
Paul J Hung
Keyword(s):  
Cell Migration ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals GMFβ controls branched actin content and lamellipodial retraction in fibroblasts

2015 ◽  
Vol 209 (6) ◽  
pp. 803-812 ◽  
Author(s):  
Elizabeth M. Haynes ◽  
Sreeja B. Asokan ◽  
Samantha J. King ◽  
Heath E. Johnson ◽  
Jason M. Haugh ◽  
...  
Keyword(s):  
Cell Migration ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Directional Migration ◽  
Pharmacological Inhibition ◽  
Primary Activity ◽  
And Migration ◽  
Important Structure

The lamellipodium is an important structure for cell migration containing branched actin nucleated via the Arp2/3 complex. The formation of branched actin is relatively well studied, but less is known about its disassembly and how this influences migration. GMF is implicated in both Arp2/3 debranching and inhibition of Arp2/3 activation. Modulation of GMFβ, a ubiquitous GMF isoform, by depletion or overexpression resulted in changes in lamellipodial dynamics, branched actin content, and migration. Acute pharmacological inhibition of Arp2/3 by CK-666, coupled to quantitative live-cell imaging of the complex, showed that depletion of GMFβ decreased the rate of branched actin disassembly. These data, along with mutagenesis studies, suggest that debranching (not inhibition of Arp2/3 activation) is a primary activity of GMFβ in vivo. Furthermore, depletion or overexpression of GMFβ disrupted the ability of cells to directionally migrate to a gradient of fibronectin (haptotaxis). These data suggest that debranching by GMFβ plays an important role in branched actin regulation, lamellipodial dynamics, and directional migration.

Functional Screening with a Live Cell Imaging-Based Random Cell Migration Assay

2011 ◽  
pp. 435-448 ◽  
Author(s):  
Wies van Roosmalen ◽  
Sylvia E. Le Dévédec ◽  
Sandra Zovko ◽  
Hans de Bont ◽  
Bob van de Water
Keyword(s):  
Cell Migration ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Functional Screening ◽  
Cell Migration Assay ◽  
Migration Assay

scholarly journals Time-lapse lens-free imaging of cell migration in diverse physical microenvironments

Lab on a Chip ◽  
2016 ◽  
Vol 16 (17) ◽  
pp. 3304-3316 ◽  
Author(s):  
Evelien Mathieu ◽  
Colin D. Paul ◽  
Richard Stahl ◽  
Geert Vanmeerbeeck ◽  
Veerle Reumers ◽  
...  
Keyword(s):  
Cell Migration ◽  
Live Cell Imaging ◽  
Phase Contrast ◽  
Cell Imaging ◽  
Time Lapse ◽  
Live Cell ◽  
Phase Contrast Microscopy ◽  
Contrast Microscopy ◽  
Coherent Illumination

Lens-free imaging using coherent illumination is established as an inexpensive and reliable alternative to conventional phase contrast microscopy for live-cell imaging applications.

scholarly journals Actin chromobody imaging reveals sub-organellar actin dynamics

2019 ◽  
Author(s):  
Cara R. Schiavon ◽  
Tong Zhang ◽  
Bing Zhao ◽  
Leonardo Andrade ◽  
Melissa Wu ◽  
...  
Keyword(s):  
Cell Migration ◽  
Fluorescence Microscopy ◽  
Actin Cytoskeleton ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Actin Dynamics ◽  
Spatiotemporal Resolution ◽  
In Cells

AbstractThe actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are difficult to detect with fluorescence microscopy. We developed an approach using fluorescent protein-tagged actin nanobodies targeted to organelle membranes to enable live cell imaging of sub-organellar actin dynamics with unprecedented spatiotemporal resolution. These probes reveal that ER-associated actin drives fission of multiple organelles including mitochondria, endosomes, lysosomes, peroxisomes, and the Golgi.

In vitro Time-lapse Live-Cell Imaging to Explore Cell Migration toward the Organ of Corti

10.3791/61947 ◽  
2020 ◽  
Author(s):  
Jeong-Eun Park ◽  
Su Hoon Lee ◽  
Dong Jun Park ◽  
Young Joon Seo ◽  
Sung Kyun Kim
Keyword(s):  
Cell Migration ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Organ Of Corti ◽  
Time Lapse ◽  
Live Cell
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